LOCUS       sequence-325815-        6116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct-expressing oNigri AAV Virus. Alias: AiP1078 - 
            pAAV-AiE2016m-minBG-oNigri-WPRE-HGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Graybuck LT, Daigle TL, Sedeno-Cortes AE, Walker M, Kalmbach B, Lenz
            GH, Morin E, Nguyen TN, Garren E, Bendrick JL, Kim TK, Zhou T, 
            Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader
            C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, 
            Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin 
            SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B
  TITLE     Enhancer viruses for combinatorial cell-subclass-specific labeling.
  JOURNAL   Neuron. 2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011.
  PUBMED    33789083
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011."
COMMENT     Sequence Label: AiP1078-pAAV-mscRE16-minBGpromoter-oNigri-WPRE...
FEATURES             Location/Qualifiers
     source          1..6116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    2243..2831
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2296..2316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2714..2725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2863..3339
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3062..3081)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3379..3519
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3379..3508
                     /label=AAV2 ITR
     rep_origin      3594..4049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3681..3700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3891..3912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4066..4085)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4185..4207
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4245..4263)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4331..4435
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4436..4504,4505..5296)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4654..4673)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5467..6055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5956..5975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtagggtg caggagaaat gtgacctcaa agtcttgttc
      181 tataactgtt ggaccttagg agagatctgt gctcagcaat ttaccaggac acccccaccc
      241 cacatgtctt gaccactgtc tggataactg gtatgcagga ccacactagg cttactcaca
      301 gtgtaaactc tcataaccat cactggagcc catcctgcct ggtagacaag gattcaacca
      361 tgactcattg tactttagtg gtgccatgct tagtcatcag gtgccctgtg ctctgacagc
      421 cgagggtcag agctggaatc acactcttgt tgtcttttaa tctctccctc cctttcttcc
      481 ttctttcttc actctgttgt gattgctcat ggaacagatc ctagctggtc tccctggcaa
      541 cctacatgat ttgagcccaa cagatggata atggggacat cgacttccaa tgtcattcaa
      601 cagaatcatt gccaagggag tctgatgagc aggcaactga gatgacaccc ttatcaatat
      661 agcttcattt tggcaatctg gagtaggtgt ttcaaaagga gagcccccac tgatgccagc
      721 aatacagaac gttcatgggc aaggagctcg ggctgggcat aaaagtcagg gcagagccat
      781 ctattgctta catttgcttc tgggatccgc cgccaccatg aagaagaaga ggaaggtgga
      841 caagcaccac ctgctgtcta cccggaccac agctcctaca gacggcagcg atttcaactc
      901 cgataacgtg accaagaacc accacgccat cctgatcgag agggccctgg ccagcgccct
      961 ggccagacct ggcctgctgc tggagacaca ctttcagcca atctaccaga gggctgacag
     1021 gctgggagtg gacctgtctg attgccccag cttcttcgtg gctacccaga ggctgctggc
     1081 ccagcaccac cagcacggag ctggactgtc tccacgcagc ctggagcagt tccacagcgc
     1141 cgtgagggtg tttacacagt ggtgtcacgt gaacaggaga accgctctgc cagcctccgc
     1201 tgacacattc gtgctgtttg ctagaagcaa cgccccccac atcaccctgt ccacactgca
     1261 catctacgtg tgggccatcc ggaagctgca cctgatcacc ggcctggtgg acccaacaga
     1321 ttctcaggct gtgaagcagc acctgagcag aatcaagaag cagaagatcg cccagttcga
     1381 caccgctcag gatcaggccg tgcctctgtc cgacgaggat taccgcaccg ctatgacact
     1441 gctgatggcc gacgatcacc caatctcttg gagggacgct accctgctgg gactggccta
     1501 ccacacaatg ctgcggcaga gcgagctggt gcgcatccag ctgacccaca tccagccacg
     1561 ctccgacggc gattggacac tggagatccc ttacaccaag acaaacaaga ccggaaggtc
     1621 tgagtacgtg acactgcccc actacctgat gcctatcctg cagagatacc tgagcctgtg
     1681 cggcgaccgc accctgacag atccaggata cctgttcgtg ccactgacca ggagcggcgt
     1741 gccccggcgc cagagaatgc acgtgacctc cacacccagg gccccttctg gagtgacccc
     1801 taggagaaag acaagaggcg gaatccagtt tgctccagtg gctccagacg ctgtgaccga
     1861 ttccatcgct gctccacctg accctgctcc aatctctgag gaggtgcagc ctgtggctcc
     1921 aaagctggtg gcccgcacac tgaagaagat cggcgagagg ctggccaccc acacaagctc
     1981 cccacaggct gatagggctt actccggaca ctctgctagg gtgggaagag ccatccacct
     2041 gctggagatc ggcgctagaa aggaggacat cgtgaaggcc ggacggtgga agtccgacat
     2101 catgtttgag cggtacaccc gccagtacga cgtgaacgat ggctacctgg ctcagatcag
     2161 gcaggctgag gacagacact ggcacgctca gaccgagcca ccagattcta caggatgaga
     2221 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2281 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2341 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2401 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2461 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     2521 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2581 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     2641 atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt
     2701 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     2761 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     2821 cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac
     2881 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     2941 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     3001 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     3061 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     3121 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     3181 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     3241 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     3301 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg
     3361 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     3421 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     3481 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     3541 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     3601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3661 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3721 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3781 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     3841 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3901 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     3961 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4021 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     4081 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4141 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4201 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     4261 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4321 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4381 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4441 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4501 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4561 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4621 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4681 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4741 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4801 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4861 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4921 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4981 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5041 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5101 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5161 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5221 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5281 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5341 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5401 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5461 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5521 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5581 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5641 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5701 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5761 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5821 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5881 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5941 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6001 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6061 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
