LOCUS       sequence-325819-        6390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To generate Adeno-Associated viruses for expression of psychLight2 
            under synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Dong C, Ly C, Dunlap LE, Vargas MV, Sun J, Hwang IW, Azinfar A, Oh 
            WC, Wetsel WC, Olson DE, Tian L
  TITLE     Psychedelic-inspired drug discovery using an engineered biosensor.
  JOURNAL   Cell. 2021 Apr 8. pii: S0092-8674(21)00374-3. doi: 
            10.1016/j.cell.2021.03.043.
  PUBMED    33915107
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 8. pii: S0092-8674(21)00374-3. doi: 10.1016/j.cell.2021.03.043."
COMMENT     Sequence Label: pAAV_hSynapsin_psychLight2
FEATURES             Location/Qualifiers
     source          1..6390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        212..659
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     1782..1803
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1909..1930)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2170..2189)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2828..3416
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2881..2901)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3299..3310)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3419..3435)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3420..3436)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3465..3586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3476..3495)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3530..3549
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3748..3877
                     /label=AAV2 ITR
     rep_origin      complement(4114..4702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4194..4213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4873..5664,5665..5733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5496..5515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5734..5838)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5864..6319)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6001..6022)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6213..6232
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatct ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga
      241 ggcggggtgg gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc
      301 ccattcccca aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag
      361 gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg
      421 cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa
      481 ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc
      541 acgcgaggcg cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact
      601 cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt
      661 cgaattcaag ctgctagcaa ggatccaccc gccaccatga agacgatcat cgccctgagc
      721 tacatcttct gcctggtgtt cgccgactac aaggacgatg atgacgccat ggacatactt
      781 tgtgaagaga atacttcact ctcttctact actaactctc ttatgcaact gaacgatgat
      841 acccgattgt actcaaacga cttcaattcc ggcgaagcga acaccagtga cgcattcaac
      901 tggactgtcg attctgaaaa cagaactaat ctgtcatgcg agggttgtct tagtccctct
      961 tgtctcagcc tgttgcacct ccaggaaaag aactggtcag cactgctcac tgcggtagtg
     1021 ataatactca ctattgctgg caatattctc gtaattatgg cagtctcctt ggagaagaaa
     1081 ctccaaaacg ccacaaatta ttttcttatg agccttgcca tcgcagatat gctcttggga
     1141 tttttggtga tgcctgtgag tatgctcacg atactgtatg gatatcgctg gcctctgccg
     1201 tctaaacttt gcgctgtgtg gatttacttg gatgtccttt tttcaactgc gagtattatg
     1261 catctttgcg ccattagtct tgataggtat gtggctatcc aaaatcctaa gcaccattcc
     1321 cgctttaata gtagaactaa ggcttttctg aaaataatag cagtgtggac catatctgtc
     1381 ggcataagca tgcctatccc cgtatttgga cttcaagatg actcaaaggt attcaaagaa
     1441 gggtcatgtc tgctggccga tgacaatttc gtgcttattg gcagcttcgt cagtttcttc
     1501 attcctttga caatcatggt gattacctac tttcttacga ttaaatcttt gcaaaagcag
     1561 ctgagctcag gttataacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
     1621 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
     1681 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
     1741 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1801 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
     1861 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1921 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     1981 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2041 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     2101 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     2161 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2221 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2281 cacaagctgg agtacaactt taatatgcat gaccaactga atgagcaaaa ggcttgtaag
     2341 gtactcggca tagtcttctt tctgtttgtg gtgatgtggt gtcccttctt tataacgaat
     2401 atcatggcag tgatctgcaa ggaatcatgc aatgaggatg tgatcggggc acttctgaac
     2461 gttttcgtgt ggatagggta tctgtcaagt gctgtgaacc cactggtcta taccttgttt
     2521 aataagacat accgctcagc cttttcacgg tatattcaat gtcagtataa ggaaaacaag
     2581 aaacctctgc aacttattct tgtgaacact atccctgccc tggcttataa gtcatcacag
     2641 ttgcagatgg gccagaaaaa aaattccaag caggacgcga agacaacaga caacgattgt
     2701 agtatggttg ccctcggcaa gcagcacagt gaagaagcga gcaaagacaa tagtgatggc
     2761 gtaaacgaaa aagtcagttg tgtattctgc tacgagaacg aggtgtaaca acctgaagct
     2821 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2881 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2941 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3001 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3061 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3121 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3181 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3241 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3301 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3361 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3421 ataccgtcga cctcgacccg ggcggccgct tcgagcagac atgataagat acattgatga
     3481 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3541 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3601 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa
     3661 cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt
     3721 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     3781 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     3841 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa tcggccaacg
     3901 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaagc
     4081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     5881 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     5941 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     6001 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     6061 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     6121 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     6181 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     6241 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     6301 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6361 tcggtgcggg cctcttcgct attacgccag
//
