LOCUS       sequence-325820-         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and an sgRNA targeting Zmat3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Bieging-Rolett KT, Kaiser AM, Morgens DW, Boutelle AM, Seoane JA, 
            Van Nostrand EL, Zhu C, Houlihan SL, Mello SS, Yee BA, McClendon J, 
            Pierce SE, Winters IP, Wang M, Connolly AJ, Lowe SW, Curtis C, Yeo 
            GW, Winslow MM, Bassik MC, Attardi LD
  TITLE     Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression 
            Program.
  JOURNAL   Mol Cell. 2020 Nov 5;80(3):452-469.e9. doi: 
            10.1016/j.molcel.2020.10.022.
  PUBMED    33157015
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10"; journalName: "Mol Cell"; date: 
            "2020-11-5- 5"; volume: "80"; issue: "3"; pages: "452-469.e9"
COMMENT     Sequence Label: Lenti-sgZmat3.51.5/Cre
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        146..221
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(309..332)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
       61 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacca
      121 aacaccggct tacacagctc ctccagtttt agagctagaa atagcaagtt aaaataaggc
      181 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt tggcgcgcct
      241 atggcttaat taacatggcg gccgcattct accgggtagg ggaggcgctt ttcccaaggc
      301 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
      361 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
      421 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
      481 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
      541 gca
//
