LOCUS       sequence-325838-       12515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) 
            Cas9 cDNA plus control-targeting sgRNA into (ES) cells by 
            recombination-mediated cassette exchange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12515)
  TITLE     Karreth lab plasmids
REFERENCE   2  (bases 1 to 12515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: cTRE-LSL-GFP-IRES-Cas9-sgCR8
FEATURES             Location/Qualifiers
     source          1..12515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..547
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(83..106)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     480..499
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    593..640
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    join(673..738,739..776)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    945..1079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1199..1333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1349..1597
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1349..1369
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1520..1539
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1618..1693
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    join(1741..1759,1760..1782,1783..1801,1802..1824,1825..1843,
                     1844..1866,1867..1885,1886..1908,1909..1927,1928..1950,
                     1951..1969,1970..1992,1993..2011)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2044..2082
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2079..2103
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(2148..2181)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          2719..2784
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2914..2934
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3359..3493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3535..3568)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3608..3617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3614..3616,3617..3619,3620..4333)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3920..3939)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4267..4288
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4341..4908
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4358..4910
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4502..4519)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4729..4748
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4945..4954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6616..9114
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             9118..9138
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9182..9754
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     rep_origin      complement(10082..10670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10162..10181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10841..11632,11633..11701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11464..11483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11702..11806)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11832..12287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11969..11990)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12181..12200
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12414..12436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12428..12445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12429..12445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12452..12471
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12452..12470
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     join(12503..12515,1..4)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(12503..12515,1..4)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gatcccccgg gctgcaggaa ttaattcgtc gacctcgaaa ttctaccggg taggggaggc
       61 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      121 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      181 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      241 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      301 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      361 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      421 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      481 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      541 cctttcgacc tgcatccatc tagatctcga tcgagcagct gaagcttatg gagaagttac
      601 tattccgaag ttcctattct ctagaaagta taggaacttc gatccccctc gatcccccgg
      661 gctgcagatc tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
      721 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtgggg
      781 atcgacggta tcgataagct tgcagatctg cgactctaga ggatctgcga ctctagagga
      841 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      901 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     1081 gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1141 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1321 tcatgtctgg atcgagttta ccatgcatga gggcctattt cccatgattc cttcatattt
     1381 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
     1441 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     1501 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     1561 cttggcttta tatatcttgt ggaaaggacg aaacaccgac atttctttcc ccactgggtt
     1621 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     1681 accgagtcgg tgcttttttg aattcgatgc atcttatcat gtctggatcg agtttaccac
     1741 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1801 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1861 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1921 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     1981 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
     2041 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     2101 atcgcctgga gacgccatcc acgctcgaga cctcttcgag ggacctaata acttcgtata
     2161 gcatacatta tacgaagtta tattaagggt tccggatcct cggggacacc aaatatggcg
     2221 atctcggcct tttcgtttct tggagctggg acatgtttgc catcgatcca tctaccacca
     2281 gaacggccgt tagatctgct gccaccgttg tttccaccga agaaaccacc gttgccgtaa
     2341 ccaccacgac ggttgttgct aaagaagctg ccaccgccac ggccaccgtt gtagccgccg
     2401 ttgttgttat tgtagttgct actgttattt ctggcacttc ttggttttcc tcttaagtga
     2461 ggaggaacat aaccattctc gttgttgtcg ttgatgctta aattttgcac ttgttcgctc
     2521 agttcagcca taatatgaaa tgcttttctt gttgttctta cggaatacca cttgccacct
     2581 atcaccacaa ctaacttttt cccgttcctc catctctttt atattttttt tctcgatcga
     2641 gggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     2701 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     2761 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     2821 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     2881 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     2941 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     3001 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     3061 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     3121 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     3181 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     3241 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3301 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3361 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3421 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3481 tcatgtctgg atctgacatg gtaagtaagc ttgggctgca ggtcgaggga cctaataact
     3541 tcgtatagca tacattatac gaagttatat taagggttcc ggatcctaag cttgatggaa
     3601 ttctcgagcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3661 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtctggcg agggcgaggg
     3721 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3781 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3841 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3901 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3961 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4021 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4081 caagcagaag aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcag
     4141 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4201 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4261 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4321 gctgtacaag taatgaattt tccgcccccc ccccctaacg ttactggccg aagccgcttg
     4381 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4441 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4501 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4561 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4621 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4681 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     4741 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     4801 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4861 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccctcgt
     4921 cgagtattga tcagtggatc cggtgccacc atggactata agrwssmcsm mrrrraswas
     4981 mrgrakswyg rtatysaykr mrwmsmmgmw gmcgayaaga wgkmcmsmaw sggcctggac
     5041 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
     5101 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
     5161 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
     5221 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
     5281 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
     5341 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
     5401 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
     5461 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
     5521 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
     5581 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
     5641 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
     5701 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
     5761 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     5821 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     5881 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     5941 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     6001 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     6061 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     6121 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     6181 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     6241 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     6301 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     6361 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     6421 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     6481 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     6541 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     6601 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     6661 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     6721 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     6781 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     6841 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     6901 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     6961 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     7021 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     7081 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     7141 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     7201 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     7261 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     7321 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     7381 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     7441 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     7501 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac
     7561 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     7621 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     7681 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     7741 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     7801 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     7861 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     7921 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     7981 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
     8041 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
     8101 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
     8161 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
     8221 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     8281 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     8341 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     8401 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     8461 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     8521 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     8581 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     8641 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
     8701 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     8761 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     8821 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     8881 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     8941 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
     9001 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
     9061 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg cgacagcccc
     9121 aagaagaaga gaaaggtgga ggccagctaa gaattcgcag tcgagcgttg agaacttcag
     9181 ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata
     9241 tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca
     9301 tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc
     9361 tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg
     9421 aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt
     9481 ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt
     9541 tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat
     9601 tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca
     9661 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
     9721 taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt
     9781 gctgtctcat cattttggca aaggattcac tcctcaggtg caggctgcct atcagaaggt
     9841 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgtt ttccctctgc
     9901 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     9961 atttattttc attgcaatag tgtgttggaa tttctcgatc gctagcatcc taggcgcgcc
    10021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10501 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    11041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    11101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    11161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    11221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11821 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
    11881 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
    11941 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
    12001 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    12061 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    12121 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    12181 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    12241 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
    12301 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    12361 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    12421 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga
    12481 gctccaccgc ggtggcggcc gctctagaac tagtg
//
