LOCUS       sequence-325842-        5306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnn1a gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnn1a
FEATURES             Location/Qualifiers
     source          1..5306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(561..588)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(680..766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     815..837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     829..846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     830..846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    862..961
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3724..3823)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3840..3859)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3841..3859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3864..3880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3864..3880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3982..4001)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3993..4802
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4060..4079)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(4949..5306,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
      541 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
      601 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
      661 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
      721 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
      781 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
      841 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
      901 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc
      961 tccgcggccg cccccttcac catgcggcat cggcacgcgg gccatgcggc atcggcacgc
     1021 gggccatgcg gcatcggcac gcgggagctg tccaccagca gcacagcaac gacgcggacg
     1081 ctcgtgacag tccaaaagaa gcatcttgtc cagtcgcccc gctctttagt ctgccgcccc
     1141 caccgccatc gataaaggag cgatccacct cctcacataa caaccgacat gtacagtcct
     1201 gtccggaccg cggcaccagg aacgacgagt gcctgccttt gaccggccac tgcagcctcg
     1261 cgcagggagc gcgcaccgac cgtcagcctt acgacaacct gccgcatccg tttccagggg
     1321 cctcatctgg ctatcagtgt cattcagagg ggagccattc tcctcagcag agatcttccg
     1381 tttccccgca gccctcccca tctgaactgc tgccgccgcc tctgccttcg cacatttcta
     1441 atacgcggga tacgcgccaa aagagtgcta attacaaaca agacatcgta cagcgtcgcg
     1501 tcggggaggg ggggcgtcga gaccaatacc agttgctgca gcaaccttgt tataatcaac
     1561 aaagggaccc ctcctttgag ccactcttgc caaactctag tcaaggacac gtaaatgagc
     1621 gcgaggaagc cgagaaggag aggcaattaa ttgagcctca ggctcagtta ctgttgggca
     1681 gcagcttgcc gatcaactac tgcatcggcg gatcggggca agtgtgcaga aacaagcagg
     1741 cgccgctgtt gcagcatcag catcagcagg ctcgcgcgca ggagcacagc ctccagcagc
     1801 agcacagtga ccgagacagc aggtaccagg aggggaaaat cgcgctctgt cagccgtatc
     1861 gatgcgagcg cgcgctggag agaaactcgt ccgtgtctag tagtgaagcc agcagccccc
     1921 cgtacggctc cagcagtcaa ctgtggccag agccccacga caaggaggag aagaaaaatc
     1981 gggctggcaa atacaaggca catgctgagc gaacaaagct gagccatgct ggatccaagg
     2041 cctccgtctc cgagagcagt cactcactcc ctcagatagc catgaacagc tgcaagtaca
     2101 atggaggagt ggtgagacca ctgggcagcc tggcggcgtc aagacgcaac ctggcggagc
     2161 atgactctga gacccagccg ctccagacgc tgcacagctc cggcctggaa gtggtcgtct
     2221 ccaagggcgg aggcgaagac cccagcaagg cgtccaatga gagcctggtc cgagagagcg
     2281 gcaacgcacc gcagaagaag aataaagata ttggctacaa actgggccac aggagggcac
     2341 tttttgagaa acgcaagcgg ctcagtgact atgccctaat attcggcatg tttgggatcg
     2401 ttgtgatggt aacagagact gaactatcct ggggggttta cactaaggaa tcttcatatt
     2461 catttgcact gaaatgcctt atcagccttt ccactgttat tttgcttggt cttataataa
     2521 tgtaccacgc tcgggaaata cagctgttca tggtggacaa tggtgcggat gactggagga
     2581 tagccatgac atacgagcgc atctttttca ttgtcctgga gctgttggtg tgtgccattc
     2641 accccatccc gggccagtac gtattcacat ggacggcccg gctggctttc acctacgtgc
     2701 cctccgtggc agacgccgac gtagacatca tcctctccat ccccatgttc cttcgcctct
     2761 acctgatagg ccgggttatg ctccttcata gcaaactgtt cacggacgca tcttcacgca
     2821 atataggtgc cctcaacaag atcaacttca acacacgctt cgtgatgaag accctcatga
     2881 ccatctgtcc gggcaccgtg ctgctggtct tcagcatctc ctcctggatc atcgcggcct
     2941 ggaccgtgcg ggtctgtgag aggtatcatg acaaacaaga agtcactagt aactttcttg
     3001 gagctatgtg gctaatttcc ataactttcc tatcaattgg ctatggagat atggtccctc
     3061 acacatactg tggaaaaggg gtctgccttc tcacgggaat catgggagct ggttgcactg
     3121 cacttgtagt cgcggtagta gccagaaagt tagaattgac aaaagctgag aaacacgtcc
     3181 acaatttcat gatggacacc caactttgta aacgagtgaa gaacacagct gccaatgtac
     3241 tcagggaaac atggctcatc tacaaacaca caaaattagt gaagaagata gatcatgcca
     3301 aagtgcgcaa acatcagcgc aagttcttgc aggccattca ccaagctcag aagctccgca
     3361 gtgtgaaaat ggagcagagg aaactcaatg accaggccaa cactctggtg gacctggcga
     3421 agacacagaa tgtgatgtat gacctggtgt cagagctgca ggagcgcagc gaggagctgg
     3481 acaagcgtat tggcacattg gaggacaagc tggacgcagt caccggcagc ctgcaggctc
     3541 tgccctgtct gatctcccaa gccataactc agcagcagca ggatcttctg gatggctttg
     3601 tgcaccgctt ccggccccca tcactgggct cagaccgttc ctggacccca tctagacggc
     3661 ggcgctcccc ctccactgcc ccgcacacct cctcggacag cggctaaaag ggtgggcgcg
     3721 ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     3781 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     3841 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     3901 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     3961 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     4021 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     4081 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     4141 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     4201 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     4261 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     4321 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     4381 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     4441 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     4501 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     4561 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     4621 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     4681 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     4741 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     4801 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     4861 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4921 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4981 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5041 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5101 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5161 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5221 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5281 gcacacagcc cagcttggag cgaacg
//
