LOCUS       sequence-325844-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish 
            loss-of-functionkcnj13 mutant, Q153H and M154I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj13-Q153H-EGFP
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(724..748)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6055..6075)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6095..6111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(6484..6646,6647..6658)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     7007..7028
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7191..7295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7296..7364,7365..8156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7514..7533)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8327..8915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8816..8835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9069..9086
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9203..9224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9239..9244,9245..9262,9263..9269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9277..9293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9282..9304
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9301..9317
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9336..9356
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9338..9356
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9393..9409
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9393..9409
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(9443..9454,9455..9642)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    10081..10215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10118..10137)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10172..10191
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10220..10239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10220..10238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(10292..11002,11003..11005,11006..11008))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(10292..11002,11003..11005,11006..11008))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10334..10355)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10683..10702
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10942..10963
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      11005..11014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    11013..11037
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgataaagg cctccagcat gagtcccaga aggatgtgca gggcaagcat gagtcccaga
       61 aggatgtgca gggcaagagg cctccagcat gagtcccaga aggatgtgca gggcaagcag
      121 cgcggtccca gaaggatgtg cagggcaaga ggcctccagc atgagtccca gaaggatgtg
      181 cagggcaagc agcatgagtc ccagaaggat gtgcagggca agccagcatg agtcccagaa
      241 ggatgtgcag ggcaagcagc gcgatggcag tgggacagtc tgcatttggg tacatggtgc
      301 catacccaat agtgagttgc gtctccaggg cgaatgaaaa agcggctgtg aacccattca
      361 catgtttaac acacagcaca tgaccgggag gaggagaatc atgatccagg acatccaggt
      421 ctccattgac ccgagccagc aggtaccata atacagcaaa cagcagccag tgaagcagga
      481 atgagccaca aaatgccaaa accacccacc gccaccgtag cgccagccat gtgccccaca
      541 agtcccgcag agcactgaaa cacgtctctc tggagcctcc tcttgtgttt cctcttgtct
      601 ggctccgacc gtctttgctg accaggcggc gacgctgagg tttgaccatc aaagggcagg
      661 aggccttttg gtcagcggtg gtgtttgtca tggtggtagg catggtgaag ggggcggccg
      721 cggagcctgc ttttttgtac aaacttgagg ctgaactgta aaagaaaggg aaactgttag
      781 aattgcactg ctgccaggga gaggcgtttc agcaagcatg tgaccatctg gagtcaactt
      841 cctgttttgc tctgcattct acagtctgac cgctggtggg cgctagtgac agcgcgcgcc
      901 tcagatatgc cagacaaagg aagtccctct gcattctctc attattacca taaaaggcaa
      961 tggtttgagc cgctttgcgg ctgccggcgg tgtggcactc agccagagtc gtagggctca
     1021 gtaaaaagga acagatttcg acattcatca caccaggacc actccctaaa ttttacacaa
     1081 atgaagtttc acatcctctt ccaccctcgc ctgcgatcaa ttacacaacc gcatttcaac
     1141 gggtgtctgc cgccgggtgt tactgcgtca cagatctgca gtcagtccac ggactcctcg
     1201 tgagcggaga cttcacacta aagcatttct ggacggtcac tctcagtcgg ttcagttcat
     1261 agtctagcct caaaaatcac cgagatcctg aaaaactgat gggctctgct tcgacagtca
     1321 cagcggctga agtgcagcca gagaactccg cagccgttat taaagtggac cacacctcta
     1381 aaattcaaat ctttcatagc ggatacaaca cttcactaac gttaacgtta atcttcagct
     1441 tcattaaaga cagagggggc tgcaggctac atttaaatag aacacataac aaaaagaaaa
     1501 ttaaccaagt taaaactaaa tgccaatacc cgttactaat aacaagatct tcgatcttat
     1561 aaagcacgaa tgagccgatg ttagattgat ggggtttacg ttacatcaga tgagagctgg
     1621 ttagccggct agctaaagta ggtcacaccc tgctgcagca ctgcctgcac aaagaacttc
     1681 ccatgccggc tatccatcaa gtgtgcaaca tgctcaccag caccctcaaa actgtagtta
     1741 aaaatcacat ttatggctgt taaagagaga ttagccagaa cacaacttta tattaagact
     1801 taacacccat ttcacaccgg cctattcaat gttcaatgtc gtgcctacga ctggtcacgt
     1861 taaattaccg acattctcac ctgccacatc cactaacgct acaaaaaagt taagatacag
     1921 ccaatattta ttaaatgtaa catagattat caaaagaacc accacttata aatcaaacct
     1981 agtagataac tacaaagaga ccgttatgaa ctgtctaaag acttcatcaa atcatattta
     2041 tccatttaca cattttactt aacattaata gatagaaact ataattaaac gcttaaaaac
     2101 tgtaaaacca actcaccttg ggtttggggg gttgtcgact taagagaatg ggttacaagc
     2161 agatgatatt ccgcactagc tgcgtgtgga ggagctcaaa gtgaggctga aaagctgggc
     2221 gcgctctttt atatgacggc acatacccgg ctctagccat aaaaggtaaa ctttcggagc
     2281 tctgtgctct gattggtcca gcgtcacaca cccggctgtc gcgtgcacat tgctacactt
     2341 gcttcttgca acttgagagt gaaagaaggg gaagcttaac tggttaaaga acaaggccat
     2401 ggcgatttcc tataaaactg gaatttttat ttgcaaattt taacacagaa tttttcgtat
     2461 ttttctgcga caacgccaag actcaattaa acttttcaaa aagtctgtgc agagttacaa
     2521 gtggcagaat gcgtcataat gatggatgcc tccatgatcc tgtgttgtgt tttcaatcta
     2581 acacaacagc agctctgtag gtcatttacc tcttgtgttc acgaaatacc gtaatacaaa
     2641 caatggggga tctaatgagt acttttccat gtataaaatg gacatttttt ttatccaaat
     2701 taggatgaca ctacttgtag tatgttgtat gagcaactag ctggtcttag ctcagtagta
     2761 atgcacttta agaaagtgct ttaataactt tgaatgaatg ggacctgcat tccatgccac
     2821 attgcttcac tatttttgtg tgtacaagtt acacaatgat gtataaagtg agtgatagca
     2881 gttacatttt tgcaagtggg cctacctcag gtttaatgtt cattagaaat gttatatgtc
     2941 aagtcttgat cttaaatagt taaattttct gttcataatt tcatgtcgtg tctatgcaga
     3001 cataacattt ctttttaaag aggtttataa ggataaatcc taagaacata attctcatta
     3061 aaagtagaaa aaaaaaacac tgaaagtgtt ggtgatcagc tacaataaat aatattttgc
     3121 acacatttca gatattgcca cactgaataa cagataaagc tgtgcttgag cagcagtgat
     3181 catgataaac tacatgtgac tttttactgt gtaacttggg agtaatgcac atggccttca
     3241 gtgggagtta taaacaaaaa cagctgattg tgttaaaagt gcttgctgat accaaggaca
     3301 atgaggtgat gtgactttta ttttgttttt ggcaaattcc aagtattttg acaagacttt
     3361 ttacaattct gagcatttta cctctaaaat atggaagttt ctcagagtaa acgttgagta
     3421 taggtaatga ctaggcccac acagaatctg tgcgcacaga aatctgcaga tttccccaga
     3481 tctttagccc atcattgagt ttatttattg acttgtgtat ttggatttgc tctttagtgt
     3541 ttggactctc agcagtaaaa attaactcac actgaactaa actgaactga acttaaactc
     3601 ttaaaaaatg gactgacaca gtttcaattt actatactaa tcctctaagt taagctgctt
     3661 tgacacaatc tacattgtaa aagcactcta gaaataaaga caaaggtgta aatatatgtt
     3721 tattcagttt ttaaatacat ttccgtaata ttattgacta atatgaaagt gttcatttga
     3781 ttacaatatg gtctgaaagt aatgttttct gtcttttaga agatatatta tgtgagagac
     3841 ttgctttgtc gaccaaataa ttggatctag gtggacttgc attgtatata tttattaaaa
     3901 gttaaaaagg tattattttt ttttatttca tatagtaagt ttttagttac gatactcccg
     3961 aaaaaatctc gcataaatca cagattgttt acaaaattct ctgcagaaaa agcaaaaaat
     4021 gtcagcagat tctgtctggc cctagctatg acccaacaga gcaaacagaa taaatacaac
     4081 gaaatttaat atacagcatc agatataaaa gtgaatgttt tatagctgtt tgtgtataga
     4141 aaaaatgaga aaaaacagac ataaacagca tcatagtatg aatgtctaaa aacttgttat
     4201 ctgcaaactg gatactcatc aataaggtct cctccttcca tcccccaaca tcttttcaaa
     4261 gaatgccaag catccatcca cacccccctt tataaacaca caggttttta taagataagc
     4321 aaatatttag caccaatctg tggttaatgt gatgtaacac acaacaaaca atgcattcag
     4381 catatccagt caattctatt ttttaaccaa ccatgcttct ctttgtaact tttgtagatt
     4441 gcacagggga tcttgttaca gcagatgtgc ttttgtgagt ccatagttcc cccgaggacc
     4501 accacccttc cctgatagca gcccactaca gccttcccat tcatctctaa ataaggtact
     4561 gtcctaatga acctcagctt tgccccatga cataagattt ccagagcaat aaagtgttgt
     4621 tgactgtcag tgtgataatg tcattgtcct ttagaatgcc aaagtctctt atctcaattc
     4681 tgataccaaa gctctcccag tacaccattc atgcataatg ataagaaaca tatttatgaa
     4741 gttgttctca aaggaacact ttttgggcgt gtcatgctgc atttcatagt ttgttgtttg
     4801 gcacccacat aaataaaaca agtgtgtttt tgataataat aataataagg gatgcaccga
     4861 taccagtatc aggttttggg tcgatacttg gttaaaatat tcttactcgt attgcacatg
     4921 aaatgctgaa accacgcacc aataccactc aacaagattt cattacatgg atgtgatttg
     4981 ttgtcctacc tgacatcgat gtcttgatgt attaatgaat gtctgatata ttaatagctt
     5041 agtctttata aaccatccgt ttataaaata cacttttaac aaatctatag gcttctgacg
     5101 aataaaccga atattcaata tgatctttga tcatgtaatg tgtaaagttg tgatggcgca
     5161 agcataactt atcctttaaa tgtccacgtt taatcggtaa tcgatacagg gttgtcaaca
     5221 acgtgcgtat attcttagct taaaatatcc ttctggtaaa gatttttgtg ttcatccttg
     5281 cgacaggcaa gaaataaaac cttctttcaa attaattaca ttgaaaccaa atccaagaag
     5341 tatcagaatc ggtactcagt attgtcgagt aacagaaact attatacttg tactcatatc
     5401 ggtttgtaaa aaagtggtat cggtttatct ctaatcattc attcattcat tcattttcaa
     5461 cttagtcctt taattaacca ggggtcacca taggggaatg aaccgccaac ttatcctgca
     5521 tatgttttac acagcagatg tccttccagt tgcaacctaa cactgggaaa cacctataca
     5581 ctctcattca cacacacaca cacactcact atggtcaatt tagcttaccc cattcaccta
     5641 tacatcatgt ctttggactt gtgaggggaa accagagcac cgggaggaaa cctacttgaa
     5701 cagggagaac atgcaaactc cacacagaac tgccaactga tccagccggg gctctaaaca
     5761 gcaacctact tgctgtgagg taattgtgcc acccatcgcg ccaccgtgat gtccatccct
     5821 aataataata atacagttta tttatagtgc gcttttctca gacccaaagc gctacaatgc
     5881 atacaaggca aacagtaaca aagcagaaca ataaaaaagc tgtaaaaaca ccacaataag
     5941 acaataaaat atgaacaata caatgtaatt attaaattga caaaaataat caacctaaat
     6001 taagaccaac agtgaaaagg tgagtcttga aaagtttctt caaactggaa ttttcaactt
     6061 ttctatacaa agttgatagc ttggcgtaat catggtcata gctgtttcct gctcgagggc
     6121 ccatctggcc tgtgtttcag acaccaggga gtctctgctc acgtttcctg ctatttgcag
     6181 cctctctatc aagactaata cacctcttcc cgcatcggct gcctgtgaga ggcttttcag
     6241 cactgcagga ttgcttttca gccccaaaag agctaggctt gacactaaca attttgagaa
     6301 tcagcttcta ctgaagttaa atctgaggtt ttacaacttt gagtagcgtg tactggcatt
     6361 agattgtctg tcttatagtt tgataattaa atacaaacag ttctaaagca ggataaaacc
     6421 ttgtatgcat ttcatttaat gttttttgag attaaaagct taaacaagaa tctctagttt
     6481 tctttcttgc ttttactttt acttccttaa tactcaagta caattttaat ggagtacttt
     6541 tttactttta ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt
     6601 tccctaagta cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgtc
     6661 aagaactcct ggacaaacct ctgacctgtg tggaacagag tggatatggg tgtctgaaca
     6721 gatattcacg tcttttgcag atcagagggc atttctggtg ttgtgcaatg acctgggtcc
     6781 aacaggtagg cgtactcgta tccaagagca ttgttggaca ggaggtagtc tccattccta
     6841 taaagaggaa ggaatggcac catgtagtag ccgtcattgt ggccaattgg tgcattggta
     6901 cgtgggtaga tggaccgggg aggctgatga gttcttagcc agcgtgggcc atcgccctga
     6961 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7021 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7081 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7141 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7201 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7261 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7321 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7381 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7441 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7501 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7561 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7621 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7681 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7741 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7801 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7861 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7921 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7981 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8041 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8101 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8161 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8221 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatgccttaa cgtgagtttt
     8281 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8341 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8401 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8461 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8521 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8581 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8641 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8701 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8761 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8821 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8881 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8941 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9001 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9061 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9121 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9181 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9241 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9301 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     9361 aaaagctgga gctccaccgc ggtggcggcc gctctagaac tagtggatct gctgggcttg
     9421 ctgaaggtag ggggtcaaga accagaggtg taaagtactt gagtaatttt acttgattac
     9481 tgtacttaag tattattttt ggggattttt actttacttg agtacaatta aaaatcaata
     9541 cttttacttt tacttaatta cattttttta gaaaaaaaag tactttttac tccttacaat
     9601 tttatttaca gtcaaaaagt acttattttt tggagatcac ttcattctat tttcccttgc
     9661 tattaccaaa ccaattgaat tgcgctgatg cccagtttaa tttaaatgtt atttattctg
     9721 cctatgaaaa tcgttttcac attatatgaa attggtcaga catgttcatt ggtcctttgg
     9781 aagtgacgtc atgtcacatc tattaccaca atgcacagca ccttgacctg gaaattaggg
     9841 aaattataac agtcaatcag tggaagaaaa tggaggaagt atgtgattca tcagcagctg
     9901 cgagcagcac agtccaaaat cagccacagg atcaagagca cccgtggccg tatcttcgca
     9961 gatctgatct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
    10021 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    10081 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10141 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10201 tatcatgtct ggatctacgt aatacgactc actatagggc tgcagaatct agaggctcga
    10261 gaggccttga attcccagat cttccaccgc ccttgtacag ctcgtccatg ccgagagtga
    10321 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
    10381 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
    10441 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
    10501 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
    10561 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
    10621 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
    10681 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
    10741 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
    10801 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
    10861 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
    10921 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
    10981 tgaacagctc ctcgcccttg ctcaccatgg tggccacttt gtacaagaaa gctgggtcgg
    11041 cgcgcccacc cttcttctcc acgcggtcgc tgccttctcc attcagctga accaccacat
    11101 gctctttatc cggtgtatca gtggtgtgtg tgttggctag tgtgagggga cagggcaccg
    11161 tgtcaaaata gcgcatgttt ggggttttgt tctggtgtac agacgtcacc ttggagaagc
    11221 agtagccata ttggatctcg tcaggcaggt aagacgtcct cttgtggtaa ccagaccctg
    11281 tgctttcctg agtcgcagtg agaaacacta caagctcaaa gtgtgtttgg ctgcttgggt
    11341 tgttgatgaa aggagtggat ggatttaaag ggtgaaagaa ggtcagaggg gagaggaaaa
    11401 gagggcaaga tctggagccc aggttgtcga tgctgaactc cagagcagtt tggtggagtt
    11461 cgtgatcgtc tcactcctcg tagaggacag cactcacgga tacgtccact agcggctgtg
    11521 gctggaggtt acacactcga aacattagac agcgctggcc tttctgctca cacaccacag
    11581 cctgagggct gaataggatc ccgtcacatc gcttctgagg ccgagagaac ttggctacaa
    11641 atgcacca
//
