LOCUS       sequence-32585-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
  TITLE     Cerulean, Venus, and VenusY67C FRET reference standards.
  JOURNAL   Biophys J. 2006 Dec 15. 91(12):L99-L101.
  PUBMED    17040988
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J. 
            2006 Dec 15. 91(12):L99-L101."
COMMENT     Sequence Label: C17V
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..836)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(168..189)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     517..536
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     776..797
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caacaattgc attcatttta tgtttcaggt ttcaggggga ggtgtgggag gttttttaaa
       61 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagttatct agatccggtg
      121 gatcccttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      181 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gcttggactg gtagctcagg
      241 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      301 tcggcgagct gcacgccgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg
      361 ccgttcttct gcttgtcggc ggtgatatag acgttgtggc tgttgtagtt gtactccagc
      421 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      481 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      541 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      601 tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc cgtagccgag ggtggtcacg
      661 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgagcttcag ggtcagcttg
      721 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      781 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctggta
      841 ccgtcgactn cagaatcgaa ctgact
//
