LOCUS       sequence-325850-       12017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and 
            EGFP". This will reflect the linkage of KCNJ13 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12017)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 12017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5317..5341
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6431..6455)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6458..7032,7033..7035,7036..7044)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6625..6642)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6852..6871
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7051..7071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7117..7119,7120..7122,7123..7836)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7117..7119,7120..7122,7123..7833)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7162..7183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7423..7442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7770..7791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7843..7862)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7844..7862)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7867..8001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7891..7910)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7945..7964
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8440..8627,8628..8639)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8673..8689)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8673..8689)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8726..8746)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8726..8744)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8765..8781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8778..8800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8789..8805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8813..8819,8820..8837,8838..8843))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8858..8879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8996..9013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9167..9755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9247..9266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9926..10717,10718..10786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10549..10568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10787..10891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11054..11075)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(11424..11435,11436..11598)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     11971..11987
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(12007..12017,1..10)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 agaaaagttg aaaattccag tttgaagaaa cttttcaaga ctcacctttt cactgttggt
       61 cttaatttag gttgattatt tttgtcaatt taataattac attgtattgt tcatatttta
      121 ttgtcttatt gtggtgtttt tacagctttt ttattgttct gctttgttac tgtttgcctt
      181 gtatgcattg tagcgctttg ggtctgagaa aagcgcacta taaataaact gtattattat
      241 tattagggat ggacatcacg gtggcgcgat gggtggcaca attacctcac agcaagtagg
      301 ttgctgttta gagccccggc tggatcagtt ggcagttctg tgtggagttt gcatgttctc
      361 cctgttcaag taggtttcct cccggtgctc tggtttcccc tcacaagtcc aaagacatga
      421 tgtataggtg aatggggtaa gctaaattga ccatagtgag tgtgtgtgtg tgtgtgaatg
      481 agagtgtata ggtgtttccc agtgttaggt tgcaactgga aggacatctg ctgtgtaaaa
      541 catatgcagg ataagttggc ggttcattcc cctatggtga cccctggtta attaaaggac
      601 taagttgaaa atgaatgaat gaatgaatga ttagagataa accgatacca cttttttaca
      661 aaccgatatg agtacaagta taatagtttc tgttactcga caatactgag taccgattct
      721 gatacttctt ggatttggtt tcaatgtaat taatttgaaa gaaggtttta tttcttgcct
      781 gtcgcaagga tgaacacaaa aatctttacc agaaggatat tttaagctaa gaatatacgc
      841 acgttgttga caaccctgta tcgattaccg attaaacgtg gacatttaaa ggataagtta
      901 tgcttgcgcc atcacaactt tacacattac atgatcaaag atcatattga atattcggtt
      961 tattcgtcag aagcctatag atttgttaaa agtgtatttt ataaacggat ggtttataaa
     1021 gactaagcta ttaatatatc agacattcat taatacatca agacatcgat gtcaggtagg
     1081 acaacaaatc acatccatgt aatgaaatct tgttgagtgg tattggtgcg tggtttcagc
     1141 atttcatgtg caatacgagt aagaatattt taaccaagta tcgacccaaa acctgatact
     1201 ggtatcggtg catcccttat tattattatt atcaaaaaca cacttgtttt atttatgtgg
     1261 gtgccaaaca acaaactatg aaatgcagca tgacacgccc aaaaagtgtt cctttgagaa
     1321 caacttcata aatatgtttc ttatcattat gcatgaatgg tgtactggga gagctttggt
     1381 atcagaattg agataagaga ctttggcatt ctaaaggaca atgacattat cacactgaca
     1441 gtcaacaaca ctttattgct ctggaaatct tatgtcatgg ggcaaagctg aggttcatta
     1501 ggacagtacc ttatttagag atgaatggga aggctgtagt gggctgctat cagggaaggg
     1561 tggtggtcct cgggggaact atggactcac aaaagcacat ctgctgtaac aagatcccct
     1621 gtgcaatcta caaaagttac aaagagaagc atggttggtt aaaaaataga attgactgga
     1681 tatgctgaat gcattgtttg ttgtgtgtta catcacatta accacagatt ggtgctaaat
     1741 atttgcttat cttataaaaa cctgtgtgtt tataaagggg ggtgtggatg gatgcttggc
     1801 attctttgaa aagatgttgg gggatggaag gaggagacct tattgatgag tatccagttt
     1861 gcagataaca agtttttaga cattcatact atgatgctgt ttatgtctgt tttttctcat
     1921 tttttctata cacaaacagc tataaaacat tcacttttat atctgatgct gtatattaaa
     1981 tttcgttgta tttattctgt ttgctctgtt gggtcatagc tagggccaga cagaatctgc
     2041 tgacattttt tgctttttct gcagagaatt ttgtaaacaa tctgtgattt atgcgagatt
     2101 ttttcgggag tatcgtaact aaaaacttac tatatgaaat aaaaaaaaat aatacctttt
     2161 taacttttaa taaatatata caatgcaagt ccacctagat ccaattattt ggtcgacaaa
     2221 gcaagtctct cacataatat atcttctaaa agacagaaaa cattactttc agaccatatt
     2281 gtaatcaaat gaacactttc atattagtca ataatattac ggaaatgtat ttaaaaactg
     2341 aataaacata tatttacacc tttgtcttta tttctagagt gcttttacaa tgtagattgt
     2401 gtcaaagcag cttaacttag aggattagta tagtaaattg aaactgtgtc agtccatttt
     2461 ttaagagttt aagttcagtt cagtttagtt cagtgtgagt taatttttac tgctgagagt
     2521 ccaaacacta aagagcaaat ccaaatacac aagtcaataa ataaactcaa tgatgggcta
     2581 aagatctggg gaaatctgca gatttctgtg cgcacagatt ctgtgtgggc ctagtcatta
     2641 cctatactca acgtttactc tgagaaactt ccatatttta gaggtaaaat gctcagaatt
     2701 gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag tcacatcacc
     2761 tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg tttataactc
     2821 ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca tgtagtttat
     2881 catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa tatctgaaat
     2941 gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt tttttttcta
     3001 cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa aaagaaatgt
     3061 tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt taagatcaag
     3121 acttgacata taacatttct aatgaacatt aaacctgagg taggcccact tgcaaaaatg
     3181 taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa aatagtgaag
     3241 caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact ttcttaaagt
     3301 gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa gtagtgtcat
     3361 cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat tagatccccc
     3421 attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag agctgctgtt
     3481 gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg acgcattctg
     3541 ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc gttgtcgcag
     3601 aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt tataggaaat
     3661 cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc aagttgcaag
     3721 aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc aatcagagca
     3781 cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc atataaaaga
     3841 gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt gcggaatatc
     3901 atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt gagttggttt
     3961 tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa aatgtgtaaa
     4021 tggataaata tgatttgatg aagtctttag acagttcata acggtctctt tgtagttatc
     4081 tactaggttt gatttataag tggtggttct tttgataatc tatgttacat ttaataaata
     4141 ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga atgtcggtaa
     4201 tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg tgaaatgggt
     4261 gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc ataaatgtga
     4321 tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg gatagccggc
     4381 atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt agctagccgg
     4441 ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg ctcattcgtg
     4501 ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt tttaacttgg
     4561 ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc tctgtcttta
     4621 atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg aaagatttga
     4681 attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg cacttcagcc
     4741 gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt tttgaggcta
     4801 gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt gaagtctccg
     4861 ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc ggcggcagac
     4921 acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg atgtgaaact
     4981 tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat ctgttccttt
     5041 ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa agcggctcaa
     5101 accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg tctggcatat
     5161 ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg cagagcaaaa
     5221 caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg gcagcagtgc
     5281 aattctaaca gtttcccttt cttttacagt tcagcctcaa gtttgtacaa aaaagcaggc
     5341 ttcaccatgg acagcagtaa ttgcaaagtt attgctcctc tcctaagtca aagataccgg
     5401 aggatggtca ccaaggatgg ccacagcaca cttcaaatgg atggcgctca aagaggtctt
     5461 gcatatcttc gagatgcttg gggaatccta atggacatgc gctggcgttg gatgatgttg
     5521 gtcttttctg cttcttttgt tgtccactgg cttgtctttg cagtgctctg gtatgttctg
     5581 gctgagatga atggtgatct ggaactagat catgatgccc cacctgaaaa ccacactatc
     5641 tgtgtcaagt atatcaccag tttcacagct gcattctcct tctccctgga gacacaactc
     5701 acaattggtt atggtaccat gttccccagt ggtgactgtc caagtgcaat cgccttactt
     5761 gccatacaaa tgctcctagg cctcatgcta gaggctttta tcacaggtgc ttttgtggcg
     5821 aagattgccc ggccaaaaaa tcgagctttt tcaattcgct ttactgacac agcagtagta
     5881 gctcacatgg atggcaaacc taatcttatc ttccaagtgg ccaacacccg acctagccct
     5941 ctaaccagtg tccgggtctc agctgtactc tatcaggaaa gagaaaatgg caaactctac
     6001 cagaccagtg tggatttcca ccttgatggc atcagttctg acgaatgtcc attcttcatc
     6061 tttccactaa cgtactatca ctccattaca ccatcaagtc ctctggctac tctgctccag
     6121 catgaaaatc cttctcactt tgaattagtt gtattccttt cagcaatgca ggagggcact
     6181 ggagaaatat gccaaaggag gacatcctac ctaccgtctg aaatcatgtt acatcactgt
     6241 tttgcatctc tgttgacccg aggttccaaa ggtgaatatc aaatcaagat ggagaatttt
     6301 gacaagactg tccctgaatt tccaactcct ctggtttcta aaagcccaaa caggactgac
     6361 ctggatatcc acatcaatgg acaaagcatt gacaattttc agatctctga aacaggactg
     6421 acagaataag acccagcttt cttgtacaaa gtggccgccc ctctccctcc ccccccccta
     6481 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     6541 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     6601 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     6661 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     6721 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     6781 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     6841 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     6901 taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt
     6961 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     7021 cacgatgata atatggccac aaccatggct ccaaagaaga agcgtaaggt aaatcgatcg
     7081 aattcaaggc ctctcgagcc tctagaaggt ggcggaatgg tgagcaaggg cgaggagctg
     7141 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     7201 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     7261 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     7321 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     7381 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     7441 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     7501 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     7561 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     7621 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     7681 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     7741 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     7801 gggatcactc tcggcatgga cgagctgtac aagtaactgc agccctatag tgagtcgtat
     7861 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     7921 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     7981 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     8041 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     8101 tatgatcctc tagatcagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct
     8161 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
     8221 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
     8281 agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa
     8341 tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc
     8401 aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag
     8461 tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat
     8521 gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc
     8581 caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg
     8641 ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag cggccgccac
     8701 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     8761 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8821 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8881 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8941 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9001 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9061 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9361 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9601 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9781 ggtctgacgc tcagtggaac gaaaactcac gttaaggcat tttggtcatg agattatcaa
     9841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
    10921 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    10981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
    11041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    11101 caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa ctcatcagcc
    11161 tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg gctactacat
    11221 ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca atgctcttgg
    11281 atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa tgccctctga
    11341 tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc acacaggtca
    11401 gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt
    11461 gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa
    11521 tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag
    11581 taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa
    11641 cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc
    11701 aaactataag acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga
    11761 tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg
    11821 ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt
    11881 gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg
    11941 tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat gattacgcca
    12001 agctatcaac tttgtat
//
