LOCUS       sequence-325859-        8856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of c-MYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8856)
  TITLE     Possemato Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1 CMYC
FEATURES             Location/Qualifiers
     source          1..8856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1748..1865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1919..2233
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1998..2016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2034..2052
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2070..2088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2105..2123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2141..2159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2250..3569
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             3588..3644
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        3672..4182
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4101..4119
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4211..4606
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4622..4675
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4676..5293,5294..5299,5300..5335,5336..5338,5339..5374,
                     5375..5377,5378..5413,5414..5422)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5462..5482)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5880..5891)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6096..6329
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6401..6522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6438..6457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6492..6511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6562..6697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6697..6768
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6769..6837,6838..7629)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6987..7006)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7800..8388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8289..8308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8542..8559
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8676..8697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8712..8717,8718..8735,8736..8742)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8750..8766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8755..8777
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8774..8790
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8774..8790
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8809..8829
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8811..8829
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca
     1741 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1801 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1861 attttcgggt ttattacagg gacagcagag atccactttg gccgcgaatc gatatgtcga
     1921 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     1981 aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2041 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     2101 tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2161 cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagaa ttggctagca tgcccctcaa cgttagcttc accaacagga
     2281 actatgacct cgactacgac tcggtgcagc cgtatttcta ctgcgacgag gaggagaact
     2341 tctaccagca gcagcagcag agcgagctgc agcccccggc gcccagcgag gatatctgga
     2401 agaaattcga gctgctgccc accccgcccc tgtcccctag ccgccgctcc gggctctgct
     2461 cgccctccta cgttgcggtc acacccttct cccttcgggg agacaacgac ggcggtggcg
     2521 ggagcttctc cacggccgac cagctggaga tggtgaccga gctgctggga ggagacatgg
     2581 tgaaccagag tttcatctgc gacccggacg acgagacctt catcaaaaac atcatcatcc
     2641 aggactgtat gtggagcggc ttctcggccg ccgccaagct cgtctcagag aagctggcct
     2701 cctaccaggc tgcgcgcaaa gacagcggca gcccgaaccc cgcccgcggc cacagcgtct
     2761 gctccacctc cagcttgtac ctgcaggatc tgagcgccgc cgcctcagag tgcatcgacc
     2821 cctcggtggt cttcccctac cctctcaacg acagcagctc gcccaagtcc tgcgcctcgc
     2881 aagactccag cgccttctct ccgtcctcgg attctctgct ctcctcgacg gagtcctccc
     2941 cgcagggcag ccccgagccc ctggtgctcc atgaggagac accgcccacc accagcagcg
     3001 actctgagga ggaacaagaa gatgaggaag aaatcgatgt tgtttctgtg gaaaagaggc
     3061 aggctcctgg caaaaggtca gagtctggat caccttctgc tggaggccac agcaaacctc
     3121 ctcacagccc actggtcctc aagaggtgcc acgtctccac acatcagcac aactacgcag
     3181 cgcctccctc cactcggaag gactatcctg ctgccaagag ggtcaagttg gacagtgtca
     3241 gagtcctgag acagatcagc aacaaccgaa aatgcaccag ccccaggtcc tcggacaccg
     3301 aggagaatgt caagaggcga acacacaacg tcttggagcg ccagaggagg aacgagctaa
     3361 aacggagctt ttttgccctg cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc
     3421 ccaaggtagt tatccttaaa aaagccacag catacatcct gtccgtccaa gcagaggagc
     3481 aaaagctcat ttctgaagag gacttgttgc ggaaacgacg agaacagttg aaacacaaac
     3541 ttgaacagct acggaactct tgtgcgtaag tcgacaccgg ttccggagcc acgaacttct
     3601 ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg tcctaggctg cagacgcgtt
     3661 gtacaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
     3721 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca
     3781 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
     3841 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
     3901 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
     3961 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc
     4021 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     4081 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     4141 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcaattcac ccgcgcgcgg
     4201 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     4261 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     4321 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     4381 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     4441 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     4501 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     4561 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcttcg aaggaagcgg
     4621 agaaggtaga ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc
     4681 tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat
     4741 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     4801 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     4861 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     4921 cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     4981 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     5041 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     5101 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     5161 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     5221 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     5281 gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc
     5341 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     5401 ccttgacatg ctccccgggt aactaagtaa ggatcgatcc aagatatcgt attcttaact
     5461 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5521 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5581 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5641 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5701 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5761 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5821 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5881 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5941 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6001 tttcgcctcg gcgtccggac tagaggtacc tttaagacca atgacttaca aggcagctgt
     6061 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6121 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6181 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6241 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6301 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6361 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6541 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6601 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6661 ctattccaga agtagtgagg aggctttttt ggaggccttt caaatatgta tccgctcatg
     6721 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6781 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6841 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6901 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6961 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7021 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7081 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7141 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7201 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7261 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7321 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7381 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7441 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7501 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7561 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7621 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7681 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7741 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7801 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7861 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7921 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7981 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8041 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8101 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8161 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8221 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8281 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8341 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8401 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8461 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8521 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8581 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8641 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8701 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8761 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     8821 cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
