LOCUS       sequence-325861-        8806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of Cyclin E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8806)
  TITLE     Possemato Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1 CCNE1
FEATURES             Location/Qualifiers
     source          1..8806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1748..1865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1919..2233
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1998..2016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2034..2052
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2070..2088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2105..2123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2141..2159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3538..3594
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        3622..4132
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4051..4069
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4161..4556
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4572..4625
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4626..5243,5244..5249,5250..5285,5286..5288,5289..5324,
                     5325..5327,5328..5363,5364..5372)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5412..5432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5830..5841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6046..6279
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6351..6472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6388..6407)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6442..6461
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6512..6647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6647..6718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6719..6787,6788..7579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7750..8338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8239..8258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8492..8509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8626..8647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8662..8667,8668..8685,8686..8692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8700..8716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8705..8727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8724..8740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8724..8740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8759..8779
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8761..8779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca
     1741 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1801 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1861 attttcgggt ttattacagg gacagcagag atccactttg gccgcgaatc gatatgtcga
     1921 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     1981 aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2041 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     2101 tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2161 cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagaa ttggctagcg aattcatgcc gagggagcgc agggagcggg
     2281 atgcgaagga gcgggacacc atgaaggagg acggcggcgc ggagttctcg gctcgctcca
     2341 ggaagaggaa ggcaaacgtg accgtttttt tgcaggatcc agatgaagaa atggccaaaa
     2401 tcgacaggac ggcgagggac cagtgtggga gccagccttg ggacaataat gcagtctgtg
     2461 cagacccctg ctccctgatc cccacacctg acaaagaaga tgatgaccgg gtttacccaa
     2521 actcaacgtg caagcctcgg attattgcac catccagagg ctccccgctg cctgtactga
     2581 gctgggcaaa tagagaggaa gtctggaaaa tcatgttaaa caaggaaaag acatacttaa
     2641 gggatcagca ctttcttgag caacaccctc ttctgcagcc aaaaatgcga gcaattcttc
     2701 tggattggtt aatggaggtg tgtgaagtct ataaacttca cagggagacc ttttacttgg
     2761 cacaagattt ctttgaccgg tatatggcga cacaagaaaa tgttgtaaaa actcttttac
     2821 agcttattgg gatttcatct ttatttattg cagccaaact tgaggaaatc tatcctccaa
     2881 agttgcacca gtttgcgtat gtgacagatg gagcttgttc aggagatgaa attctcacca
     2941 tggaattaat gattatgaag gcccttaagt ggcgtttaag tcccctgact attgtgtcct
     3001 ggctgaatgt atacatgcag gttgcatatc taaatgactt acatgaagtg ctactgccgc
     3061 agtatcccca gcaaatcttt atacagattg cagagctgtt ggatctctgt gtcctggatg
     3121 ttgactgcct tgaatttcct tatggtatac ttgctgcttc ggccttgtat catttctcgt
     3181 catctgaatt gatgcaaaag gtttcagggt atcagtggtg cgacatagag aactgtgtca
     3241 agtggatggt tccatttgcc atggttataa gggagacggg gagctcaaaa ctgaagcact
     3301 tcaggggcgt cgctgatgaa gatgcacaca acatacagac ccacagagac agcttggatt
     3361 tgctggacaa agcccgagca aagaaagcca tgttgtctga acaaaatagg gcttctcctc
     3421 tccccagtgg gctcctcacc ccgccacaga gcggtaagaa gcagagcagc gggccggaaa
     3481 tggcgtgacc accccatcct tctccaccaa agacagttgc tcgacaccgg ttccggagcc
     3541 acgaacttct ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg tcctaggctg
     3601 cagacgcgtt gtacaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt
     3661 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     3721 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     3781 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     3841 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     3901 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     3961 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     4021 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     4081 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcaattcac
     4141 ccgcgcgcgg gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg
     4201 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     4261 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     4321 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     4381 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     4441 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     4501 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcttcg
     4561 aaggaagcgg agaaggtaga ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg
     4621 gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg
     4681 gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     4741 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     4801 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     4861 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
     4921 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     4981 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     5041 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     5101 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     5161 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     5221 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
     5281 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     5341 acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc aagatatcgt
     5401 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5461 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5521 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5581 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5641 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5701 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5761 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5821 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5881 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5941 tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca atgacttaca
     6001 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6061 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6121 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6181 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6241 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6301 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6361 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6421 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6481 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6541 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt caaatatgta
     6661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8401 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8461 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8521 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8581 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8641 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     8701 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     8761 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
