LOCUS       sequence-32588-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
  TITLE     Cerulean, Venus, and VenusY67C FRET reference standards.
  JOURNAL   Biophys J. 2006 Dec 15. 91(12):L99-L101.
  PUBMED    17040988
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J. 
            2006 Dec 15. 91(12):L99-L101."
COMMENT     Sequence Label: C32V
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    121..242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(132..151)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     186..205
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(368..619)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(410..431)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
       61 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttacgcct
      121 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      181 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      241 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      301 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      361 cgctttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      421 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tggtagctca
      481 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      541 ggtcggcgag ctgcacgccg ccgtcctcga tgttgtggcg gatcttgaag ttggccttga
      601 tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg gctgttgtag ttgtactcca
      661 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      721 tcaccagggt gt
//
