LOCUS       sequence-32591-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Sells MA, Knaus UG, Bagrodia S, Ambrose DM, Bokoch GM, Chernoff J
  TITLE     Human p21-activated kinase (Pak1) regulates actin organization in 
            mammalian cells.
  JOURNAL   Curr Biol. 1997 Mar 1. 7(3):202-10.
  PUBMED    9395435
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1997 Mar 1. 7(3):202-10."
COMMENT     Sequence Label: pCMV6M-PAK1 H83L H86L K299R
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(635..652)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccgtcagaat taattcagat ctggtaccac gcgtatcgat aagcttcgat cgtcgacatg
       61 gaacagaaac tcatctctga agaggatctg tctagaggat ccatgtcaaa taacggccta
      121 gacattcaag acaaaccccc agcccctccg atgagaaata ccagcactat gattggagcc
      181 ggcagcaaag atgctggaac cctaaaccat ggttctaaac ctctgcctcc aaacccagag
      241 gagaagaaaa agaaggaccg attttaccga tccattttac ctggagataa aacaaataaa
      301 aagaaagaga aagagcggcc agagatttct ctcccttcag attttgagct cacaattctt
      361 gtcggttttg atgctgtcac aggggagttt acgggaatgc cagagcagtg ggcccgcttg
      421 cttcagacat caaatatcac taagtcggag cagaagaaaa acccgcaggc tgttctggat
      481 gtgttggagt tttacaactc gaagaagaca tccaacagcc agaaatacat gagctttaca
      541 gataagtcag ctgaggatta caattcttct aatgccttga atgtgaaggc tgtgtctgag
      601 actcctgcag tgccaccagt ttcagaagat gaggatgatg atgatgatga tgctacccca
      661 ccaccagtga ttgctccacg cccagnnnnn ncaaaatctg tatacacacg gtctgtgatt
      721 gaaccacttc ctgtcactcc nactcgggac gtggctacat ctcccatttc acctactgaa
      781 aataacacca ctccaccaga tgctttgacc cggaatactg agaagcagaa naagaagcct
      841 aaaatgtctg atgaggagat cttggagaaa ttacgaagca tagtgagtgt gggcgatcct
      901 aagaagaaat atacacggtt tgagaanatt ggacaag
//
