LOCUS       sequence-32593-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin 
            JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM
  TITLE     Modification of superoxide dismutase 1 (SOD1) properties by a GFP 
            tag--implications for research into amyotrophic lateral sclerosis 
            (ALS).
  JOURNAL   PLoS One. 2010 . 5(3):e9541.
  PUBMED    20221404
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010 . 5(3):e9541."
COMMENT     Sequence Label: pF152 pcDNA3.1(+)SOD1A4V
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(649..666)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    655..766
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc
      121 actagtccag tgtggtggaa ttccatggcg acgaaggttg tgtgcgtgct gaagggcgac
      181 ggcccagtgc agggcatcat caatttcgag cagaaggaaa gtaatggacc agtgaaggtg
      241 tggggaagca ttaaaggact gactgaaggc ctgcatggat tccatgttca tgagtttgga
      301 gataatacag caggctgtac cagtgcaggt cctcacttta atcctctatc cagaaaacac
      361 ggtgggccaa aggatgaaga gaggcatgtt ggagacttgg gcaatgtgac tgctgacaaa
      421 gatggtgtgg ccgatgtgtc tattgaagat tctgtgatct cactctcagg agaccattgc
      481 atcattggcc gcacactggt ggtccatgaa aaagcagatg acttgggcaa aggtggaaat
      541 gaagaaagta caaagacagg aaacgctgga agtcgtttgg cttgtggtgt aattgggatc
      601 gcccaataac gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
      661 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      721 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
      781 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
      841 acaatagca
//
