LOCUS       sequence-325937-       11284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Liao YC, Fernandopulle MS, Wang G, Choi H, Hao L, Drerup CM, Patel 
            R, Qamar S, Nixon-Abell J, Shen Y, Meadows W, Vendruscolo M, Knowles
            TPJ, Nelson M, Czekalska MA, Musteikyte G, Gachechiladze MA, 
            Stephens CA, Pasolli HA, Forrest LR, St George-Hyslop P, 
            Lippincott-Schwartz J, Ward ME
  TITLE     RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, 
            Using Annexin A11 as a Molecular Tether.
  JOURNAL   Cell. 2019 Sep 19;179(1):147-164.e20. doi: 
            10.1016/j.cell.2019.08.050.
  PUBMED    31539493
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08"; journalName: "Cell"; date: "2019-09-19- 
            19"; volume: "179"; issue: "1"; pages: "147-164.e20"
COMMENT     Sequence Label: pLEX-PGK-LAMP1-EYFP
FEATURES             Location/Qualifiers
     source          1..11284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2717..3227
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3146..3164
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3241..3250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3245..3254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4531..4533,4534..4536,4537..5250)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4576..4597)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4837..4856)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5184..5205
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5259..5847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5312..5332)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5730..5741)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6054..6287
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6388..6405)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6394..6618
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6664..7092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6751..6770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6961..6982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7101..7121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7106..7435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7286..7421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7348..7367
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7484..8509
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7484..8509
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8639..8760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8809..8825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8809..8825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8822..8844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8833..8849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8857..8863,8864..8881,8882..8887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8902..8923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9040..9057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9211..9799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9291..9310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9970..10761,10762..10830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10593..10612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10831..10935)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10983..11117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccacgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     2761 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     2821 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     2881 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     2941 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     3001 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     3061 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     3121 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     3181 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg atccgcggcc
     3241 gccaccatgg cggcccccgg cagcgcccgg cggccgctgc tcctgttgct gctggcaggc
     3301 cttgcacaca gcgccccagc actgttcgag gtgaaagaca acaacggcac agcgtgtata
     3361 atggccagct tctctgcctc ctttctgacc acctatgagg ctggacatgt ttctaaggtc
     3421 tcgaatatga ccctgccagc ctctgcagaa gtcctgaaga atagcagctc ttgtggtgaa
     3481 aagaatgctt ctgagcccac cctcgcaatc acctttggag aaggatattt actgaaactc
     3541 accttcacaa aaaacacaac acgttacagt gtccagcaca tgtatttcac atataacctg
     3601 tcagacacac aattctttcc caatgccagc tccaaagggc ccgacactgt ggattccaca
     3661 actgacatca aggcagacat caacaaaaca taccgatgtg tcagcgacat cagggtctac
     3721 atgaagaatg tgaccattgt gctctgggac gctactatcc aggcctacct gccgagtagc
     3781 aacttcagca aggaagagac acgctgccca caggatcaac cttccccaac tactgggcca
     3841 cccagcccct caccaccact tgtgcccaca aaccccagtg tgtccaagta caatgtgact
     3901 ggtgacaatg gaacctgcct gctggcctct atggcactgc aactcaacat cacctacatg
     3961 aagaaggaca acacgactgt gaccagagca ttcaacatca acccaagtga caaatatagt
     4021 gggacttgcg gtgcccagtt ggtgaccctg aaggtgggga acaagagcag agtcctggag
     4081 ctgcagtttg ggatgaatgc cacttctagc ctgtttttcc tgcaaggagt tcagttgaac
     4141 atgactcttc ctgatgccat agagcccacg ttcagcacct ccaactattc cctgaaagct
     4201 cttcaggcca gtgtcggcaa ctcatacaag tgcaacagtg aggagcacat ctttgtcagc
     4261 aaggcgctcg ccctcaatgt cttcagcgtg caagtccagg ctttcagggt agaaagtgac
     4321 aggtttgggt ctgtggaaga gtgtgtacag gacggtaaca acatgctgat ccccattgct
     4381 gtgggcgggg ccctggcagg gctggtcctc atcgtcctca tcgcctacct catcggcagg
     4441 aagaggagtc acgcgggcta tcagaccatc tcggaattcg gctccaccgg ctccaccggc
     4501 tccaccggcg cggatccacc ggtcgcccat atggtgagca agggcgagga gctgttcacc
     4561 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4621 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4681 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag
     4741 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4801 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4861 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4921 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4981 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     5041 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5101 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     5161 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5221 actctcggca tggacgagct gtacaagtaa cgcgtaacaa tcaacctctg gattacaaaa
     5281 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5341 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5401 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5461 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5521 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5581 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5641 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     5701 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5761 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5821 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     5881 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     5941 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     6001 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     6061 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     6121 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6181 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6241 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     6301 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     6361 ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6421 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6481 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6541 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6601 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6661 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6781 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6841 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6901 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6961 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7021 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7081 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7201 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7261 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7321 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7381 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7441 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa agcctgaact
     7501 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     7561 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     7621 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     7681 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     7741 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     7801 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     7861 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     7921 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     7981 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     8041 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     8101 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     8161 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     8221 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     8281 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     8341 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     8401 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     8461 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtgctacg
     8521 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8581 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     8641 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8701 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8761 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8821 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8881 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8941 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9001 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10081 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10261 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10921 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    10981 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    11041 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    11101 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    11161 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    11221 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11281 tagt
//
