LOCUS       sequence-325940-       10538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Hammer BK, Swanson MS
  TITLE     Co-ordination of legionella pneumophila virulence with entry into 
            stationary phase by ppGpp.
  JOURNAL   Mol Microbiol. 1999 Aug;33(4):721-31.
  PUBMED    10447882
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol."; date: "1999-08"; volume: "33(4)"; pages: "721-31"
COMMENT     Sequence Label: pMMBGent
FEATURES             Location/Qualifiers
     source          1..10538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            1823..1910
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      2252..2646
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     primer_bind     3706..3725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        join(4126..4131,4132..4148,4149..4154)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4343..4876
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(5653..5744)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5763..5790)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5882..5968)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6121..6138
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     misc_feature    6171..6227
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6233..6255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6244..6260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6268..6274,6275..6290,6291..6296))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        6526..6603
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6604..7686
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6623..6642)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(7963..8814)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8801..9640)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(join(10151..10538,1..584))
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 cgctgggcct gctcgatctg ctggccagcc tgctgcacca gcgccgggcc agcggtggcg
       61 gtcttgccct tggattcacg cagcagcacc cacggctgat aaccggcgcg ggtggtgtgc
      121 ttgtccttgc ggttggtgaa gcccgccaag cggccatagt ggcggctgtc ggcgctggcc
      181 gggtcggcgt cgtactcgct ggccagcgtc cgggcaatct gcccccgaag ttcaccgcct
      241 gcggcgtcgg ccaccttgac ccatgcctga tagttcttcg ggctggtttc cactaccagg
      301 gcaggctccc ggccctcggc tttcatgtca tccaggtcaa actcgctgag gtcgtccacc
      361 agcaccagac catgccgctc ctgctcggcg ggcctgatat acacgtcatt gccctgggca
      421 ttcatccgct tgagccatgg cgtgttctgg agcacttcgg cggctgacca ttcccggttc
      481 atcatctggc cggtggtggc gtccctgacg ccgatatcga agcgctcaca gcccatggcc
      541 ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct tcatcgggcc accaagcgca
      601 gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc gagcaagagc aacgatgcga
      661 tcagcagcac caccgtaggc atcatggaag ccagcatcac ggttagccat agcttccagt
      721 gccacccccg cgacgcgctc cgggcgctct gcgcggcgct gctcacctcg gcggctacct
      781 cccgcaactc tttggccagc tccacccatg ccgcccctgt ctggcgctgg gctttcagcc
      841 actccgccgc ctgcgcctcg ctggcctgct gggtctggct catgacctgc cgggcttcgt
      901 cggccagtgt cgccatgctc tgggccagcg gttcgatctg ctccgctaac tcgttgatgc
      961 ctctggattt cttcactctg tcgattgcgt tcatggtcta ttgcctcccg gtattcctgt
     1021 aagtcgatga tctgggcgtt ggcggtgtcg atgttcaggg ccacgtctgc ccggtcggtg
     1081 cggatgcccc ggccttccat ctccaccacg ttcggcccca ggtgaacacc gggcaggcgc
     1141 tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg cgtcgtggcc agcccgctct
     1201 aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct gctcaagcca tgccttgggc
     1261 ttgagcgctt cggtcttctg tgccccgccc ttctccgggg tcttgccgtt gtaccgcttg
     1321 aaccactgag cggcgggccg ctcgatgccg tcattgatcc gctcggagat catcaggtgg
     1381 cagtgcgggt tctcgccgcc accggcatgg atggccagcg tatacggcag gcgctcggca
     1441 ccggtcaggt gctgggcgaa ctcggacgcc agcgccttct gctggtcgag ggtcagctcg
     1501 accggcaggg caaattcgac ctccttgaac agccgcccat tggcgcgttc atacaggtcg
     1561 gcagcatccc agtagtcggc gggccgctcg acgaactccg gcatgtgccc ggattcggcg
     1621 tgcaagactt catccatgtc gcgggcatac ttgccttcgc gctggatgta gtcggccttg
     1681 gccctggccg attggccgcc cgacctgctg ccggttttcg ccgtaaggtg ataaatcgcc
     1741 atgctgcctc gctgttgctt ttgcttttcg gctccatgca atggccctcg gagagcgcac
     1801 cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa accggtaagt gcgccctccc
     1861 ctacaaagta gggtcgggat tgccgccgct gtgcctccat gatagcctac gagacagcac
     1921 attaacaatg gggtgtcaag atggttaagg ggagcaacaa ggcggcggat cggctggcca
     1981 agctcgaaga acaacgagcg cgaatcaatg ccgaaattca gcgggtgcgg gcaagggaac
     2041 agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct ggtgggggcc atgattttgg
     2101 ccaaggtgaa cagcagcgag tggccggagg atcggctcat ggcggcaatg gatgcgtacc
     2161 ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc acgccagaag gatgagccgg
     2221 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt
     2281 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg
     2341 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta
     2401 gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca
     2461 gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg
     2521 atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg
     2581 tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg
     2641 ggttcgtgac agttagtacg ggagtgacgg gcactggctg gcaatgtcta gcaacggcag
     2701 gcatttcggc tgagggtaaa agaactttcc gctaagcgat agactgtatg taaacacagt
     2761 attgcaagga cgcggaacat gcctcatgtg gcggccagga cggccagccg ggatcgggat
     2821 actggtcgtt accagagcca ccgacccgag caaacccttc tctatcagat cgttgacgag
     2881 tattacccgg cattcgctgc gcttatggca gagcagggaa aggaattgcc gggctatgtg
     2941 caacgggaat ttgaagaatt tctccaatgc gggcggctgg agcatggctt tctacgggtt
     3001 cgctgcgagt cttgccacgc cgagcacctg gtcgctttca gaaatcaatc taaagtatat
     3061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     3721 atagcagaac tttaattccg ggatcctcta gagtcgacct gcaggtcgac tctagagaca
     3781 atgccaagaa cagatccgtc agcttgcatg cccgttccat acagaagctg ggcgaacaaa
     3841 cgatgctcgc cttccagaaa accgaggatg cgaaccactt catccggggt cagcaccacc
     3901 ggcaagcgcc gcgacggccg aggtcttccg atctcctgaa gccagggcag atccgtgcac
     3961 agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa
     4021 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt
     4081 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
     4141 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     4201 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     4261 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     4321 ttgatgttat ggagcagcaa cgatgttacg cagcagcaac gatgttacgc agcagggcag
     4381 tcgccctaaa acaaagttag gtggctcaag tatgggcatc attcgcacat gtaggctcgg
     4441 ccctgaccaa gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga
     4501 cgtagccacc tactcccaac atcagccgga ctccgattac ctcgggaact tgctccgtag
     4561 taagacattc atcgcgcttg ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc
     4621 ttacgttctg cccaagtttg agcagccgcg tagtgagatc tatatctatg atctcgcagt
     4681 ctccggcgag caccggaggc agggcattgc caccgcgctc atcaatctcc tcaagcatga
     4741 ggccaacgcg cttggtgctt atgtgatcta cgtgcaagca gattacggtg acgatcccgc
     4801 agtggctctc tatacaaagt tgggcatacg ggaagaagtg atgcactttg atatcgaccc
     4861 aagtaccgcc acctaacaat tcgttcaagc cgagatcggc ttcccggccg cggagttgtt
     4921 cggtaaattg tcacaacgcc gcggccgcaa agcgctccgg cttaactcag gcgttagtcc
     4981 catcacccgc tttatgcgcg cagccactta tttcagcgct ccgccctgtt ccaaaggctt
     5041 tctagccttt tgggcctcca gcgcttgtgc agcaagcgcc accagctatc cttcttgcag
     5101 cgctgcgccg ctttcgtggt ccagcggttt ttcttgggct gcgcccttct tcaaggccgg
     5161 gcgctccctt ttggccttcg ggtctaactc tgcggtcaag cggacccgca ttctgcgggc
     5221 cgcttacctt ggccgttaga catcatgagg gtagcggtga ccatcgaaat ttcgaaccaa
     5281 ctatcagagg tgctaagcgt cattgagcgc catctggaat caacgttgct ggccgtgcat
     5341 ttgtacggct ccgcagtgga tggcggcctg aagccataca gcgatattga tttgttggtt
     5401 actgtggccg taaagctgac ggatctagag gatcccggaa ttaaaagtgc tcatcattgg
     5461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5641 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5701 catgagcgga tacatatttg aatgtattta gaaaaataaa caaaagagtt tgtagaaacg
     5761 caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca gtttatggcg
     5821 ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg
     5881 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     5941 ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga
     6001 gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt
     6061 gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct
     6121 gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc aagcttgcat
     6181 gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattctgt ttcctgtgtg
     6241 aaattgttat ccgctcacaa ttccacacat tatacgagcc gatgattaat tgtcaacagc
     6301 tcatttcaga atatttgcca gaaccgttat gatgtcggcg caaaaaacat tatccagaac
     6361 gggagtgcgc cttgagcgac acgaattatg cagtgattta cgacctgcac agccatacca
     6421 cagcttccga tggctgcctg acgccagaag cattggtgca ccgtgcagtc gataagcccg
     6481 gatcagcttg caattcgcgc gcgaaggcga agcggcattt acgttgacac catcgaatgg
     6541 tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
     6601 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     6661 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     6721 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
     6781 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
     6841 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
     6901 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
     6961 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
     7021 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
     7081 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
     7141 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
     7201 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
     7261 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
     7321 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
     7381 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
     7441 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     7501 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     7561 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     7621 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7681 cagtgagcgc aacgcaatta atgtaagtta gcgcgaattg caagctggcc gacgcgctgg
     7741 gctacgtctt gctggcgttc gggagcagaa gagcatacat ctggaagcaa agccaggaaa
     7801 gcggcctatg gagctgtgcg gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc
     7861 ttttctgagc atggtatttt tcatggtatt accaattagc aggaaaataa gccattgaat
     7921 ataaaagata aaaatgtctt gtttacaata gagtgggggg ggtcagcctg ccgccttggg
     7981 ccgggtgatg tcgtacttgc ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag
     8041 ctccggcaac gcctcgcgca cccgctggcg gcgcttgcgc atggtcgaac cactggcctc
     8101 tgacggccag acatagccgc acaaggtatc tatggaagcc ttgccggttt tgccggggtc
     8161 gatccagcca cacagccgct ggtgcagcag gcgggcggtt tcgctgtcca gcgcccgcac
     8221 ctcgtccatg ctgatgcgca catgctggcc gccacccatg acggcctgcg cgatcaaggg
     8281 gttcagggcc acgtacaggc gcccgtccgc ctcgtcgctg gcgtactccg acagcagccg
     8341 aaacccctgc cgcttgcggc cattctgggc gatgatggat accttccaaa ggcgctcgat
     8401 gcagtcctgt atgtgcttga gcgccccacc actatcgacc tctgccccga tttcctttgc
     8461 cagcgcccga tagctacctt tgaccaccat ggcatcagcg gtgacggcct cccacttggg
     8521 ttccaggaac agccggagct gccgtccgcc ttcggtcttg ggttccgggc caagcactag
     8581 gccattaggc ccagccatgg ccaccagccc ttgcaggatg cgcagatcat cagcgcccag
     8641 cggctccggg ccgctgaact cgatccgctt gccgtcgccg tagtcatacg tcacgtccag
     8701 cttgctgcgc ttgcgctcgc cccgcttgag ggcacggaac aggccggggg ccagacagtg
     8761 cgccgggtcg tgccggacgt ggctgaggct gtgcttgttc ttaggcttca ccacggggca
     8821 cccccttgct cttgcgctgc ctctccagca cggcgggctt gagcaccccg ccgtcatgcc
     8881 gcctgaacca ccgatcagcg aacggtgcgc catagttggc cttgctcaca ccgaagcgga
     8941 cgaagaaccg gcgctggtcg tcgtccacac cccattcctc ggcctcggcg ctggtcatgc
     9001 tcgacaggta ggactgccag cggatgttat cgaccagtac cgagctgccc cggctggcct
     9061 gctgctggtc gcctgcgccc atcatggccg cgcccttgct ggcatggtgc aggaacacga
     9121 tagagcaccc ggtatcggcg gcgatggcct ccatgcgacc gatgacctgg gccatggggc
     9181 cgctggcgtt ttcttcctcg atgtggaacc ggcgcagcgt gtccagcacc atcaggcggc
     9241 ggccctcggc ggcgcgcttg aggccgtcga accactccgg ggccatgatg ttgggcaggc
     9301 tgccgatcag cggctggatc agcaggccgt cagccacggc ttgccgttcc tcggcgctga
     9361 ggtgcgcccc aagggcgtgc aggcggtgat gaatggcggt gggcgggtct tcggcgggca
     9421 ggtagatcac cgggccggtg ggcagttcgc ccacctccag cagatccggc ccgcctgcaa
     9481 tctgtgcggc cagttgcagg gccagcatgg atttaccggc accaccgggc gacaccagcg
     9541 ccccgaccgt accggccacc atgttgggca aaacgtagtc cagcggtggc ggcgctgctg
     9601 cgaacgcctc cagaatattg ataggcttat gggtagccat tgattgcctc ctttgcaggc
     9661 agttggtggt taggcgctgg cggggtcact acccccgccc tgcgccgctc tgagttcttc
     9721 caggcactcg cgcagcgcct cgtattcgtc gtcggtcagc cagaacttgc gctgacgcat
     9781 ccctttggcc ttcatgcgct cggcatatcg cgcttggcgt acagcgtcag ggctggccag
     9841 caggtcgccg gtctgcttgt ccttttggtc tttcatatca gtcaccgaga aacttgccgg
     9901 ggccgaaagg cttgtcttcg cggaacaagg acaaggtgca gccgtcaagg ttaaggctgg
     9961 ccatatcagc gactgaaaag cggccagcct cggccttgtt tgacgtataa ccaaagccac
    10021 cgggcaacca atagcccttg tcacttttga tcaggtagac cgaccctgaa gcgctttttt
    10081 cgtattccat aaaaccccct tctgtgcgtg agtactcata gtataacagg cgtgagtacc
    10141 aacgcaagca ctacatgctg aaatctggcc cgcccctgtc catgcctcgc tggcggggtg
    10201 ccggtgcccg tgccagctcg gcccgcgcaa gctggacgct gggcagaccc atgaccttgc
    10261 tgacggtgcg ctcgatgtaa tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc
    10321 tggcctcggc catggccttg ccgatttcct cggcactgcg gccccggctg gccagcttct
    10381 gcgcggcgat aaagtcgcac ttgctgaggt catcaccgaa gcgcttgacc agcccggcca
    10441 tctcgctgcg gtactcgtcc agcgccgtgc gccggtggcg gctaagctgc cgctcgggca
    10501 gttcgaggct ggccagcctg cgggccttct cctgctgc
//
