LOCUS       sequence-32595-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin 
            JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM
  TITLE     Modification of superoxide dismutase 1 (SOD1) properties by a GFP 
            tag--implications for research into amyotrophic lateral sclerosis 
            (ALS).
  JOURNAL   PLoS One. 2010 . 5(3):e9541.
  PUBMED    20221404
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010 . 5(3):e9541."
COMMENT     Sequence Label: pF154 pcDNA3.1(+)SOD1G85R
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(634..651)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    640..751
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agctggctag cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg
      121 tggaattcca tggcgacgaa ggccgtgtgc gtgctgaagg gcgacggccc agtgcagggc
      181 atcatcaatt tcgagcagaa ggaaagtaat ggaccagtga aggtgtgggg aagcattaaa
      241 ggactgactg aaggcctgca tggattccat gttcatgagt ttggagataa tacagcaggc
      301 tgtaccagtg caggtcctca ctttaatcct ctatccagaa aacacggtgg gccaaaggat
      361 gaagagaggc atgttggaga cttgcgtaat gtgactgctg acaaagatgg tgtggccgat
      421 gtgtctattg aagattctgt gatctcactc tcaggagacc attgcatcat tggccgcaca
      481 ctggtggtcc atgaaaaagc agatgacttg ggcaaaggtg gaaatgaaga aagtacaaag
      541 acaggaaacg ctggaagtcg tttggcttgt ggtgtaattg ggatcgccca ataacgctcg
      601 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
      661 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      721 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagt
//
