LOCUS       sequence-325951-        8756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BFP-preSTOP-GFP reporter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Liu Y, Mao S, Huang S, Li Y, Chen Y, Di M, Huang X, Lv J, Wang X, Ge
            J, Shen S, Zhang X, Liu D, Huang X, Chi T
  TITLE     REPAIRx, a specific yet highly efficient programmable A > I RNA base
            editor.
  JOURNAL   EMBO J. 2020 Nov 16;39(22):e104748. doi: 10.15252/embj.2020104748. 
            Epub 2020 Oct 15.
  PUBMED    33058207
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2020-11-16"; volume: "39(22):e104748. doi"; pages: " 
            10.15252/embj.2020104748. Epub 2020 Oct 15"
COMMENT     Sequence Label: TC1405
FEATURES             Location/Qualifiers
     source          1..8756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   250..284
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        619..922
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        923..1126
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1077..1097
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1160..1858
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             1868..1924
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2141..2194
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2201..2203,2204..2917)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2201..2203,2204..2914)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2243..2264)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2504..2523)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2851..2872
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2958..3182
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3215..3248
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3275..3298)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3672..3691
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3753..4556
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4594..4705
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4793..4826
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   5041..5103
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5188..5207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5189..5207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5214..5231)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5214..5230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5223..5245)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5371..5800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5458..5477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5669..5690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6144..6938
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIEQARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(6956..7024,7025..7816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7174..7193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7987..8575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8476..8495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8729..8746
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acacaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
      301 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
      361 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
      421 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
      481 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
      541 tttcttgtta tagatatcat taattaaata gtaatcaatt acggggtcat tagttcatag
      601 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      661 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      721 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      781 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
      841 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
      901 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
      961 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1021 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     1081 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agaggtcgtt tagtgaaccg
     1141 tcagatcacg cgtgccacca tgagcgagct gattaaggag aacatgcaca tgaagctgta
     1201 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     1261 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     1321 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     1381 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     1441 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     1501 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     1561 gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct
     1621 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat
     1681 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     1741 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     1801 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatgg
     1861 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     1921 accggctgag caggctcgtt gggaagcagc ctccaaagaa accatcaaga aaaccaccaa
     1981 gccctgtccc cgctgccatg taccagtgga aaaaaatgga ggctgcatgc acatgaagtg
     2041 tccgcagccc cagtgcaggc tcgagtggtg ctggaactgt ggctgcgagt agaaccgcgt
     2101 ctgcatgggg gaccactggt tcgacgtgag cggttctggt gaagggcgag gttccttgct
     2161 gacctgtggc gatgtagaag aaaatcccgg ccccggatcc gtgagcaagg gcgaggagct
     2221 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2281 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2341 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     2401 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2461 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2521 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2581 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2641 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2701 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2761 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2821 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2881 cgggatcact ctcggcatgg acgagctgta caagtaaacc ggttaggggc ccgtttaaac
     2941 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3001 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3061 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3121 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3181 ggcggcgcgt ccagtgtgct ggaattcggc ttcaacaact tcgtataatg tatgctatac
     3241 gaagttatca gtacttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     3301 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     3361 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     3421 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     3481 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     3541 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     3601 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     3661 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     3721 ctcatctccg ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat
     3781 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     3841 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     3901 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     3961 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4021 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4081 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4141 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4201 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4261 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     4321 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4381 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4441 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4501 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     4561 atcaattctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4621 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4681 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4741 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg caacaacttc
     4801 gtataatgta tgctatacga agttatcagt acaagccgaa ttctgcagat atccatcggc
     4861 gcgcctaaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     4921 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     4981 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     5041 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     5101 gggttaaggc cggccaataa gggcgaattc tgcagatatc catcacactg gcggccgctc
     5161 gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     5221 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5281 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5341 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     5401 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5461 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5521 atcggggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5581 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5641 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5701 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5761 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat tcagggcgca
     5821 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     5881 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     5941 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     6001 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     6061 agtggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag
     6121 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     6181 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     6241 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     6301 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     6361 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     6421 tattgggcga agtgccgggg caggatctcc tgtcatccca ccttgctcct gccgagaaag
     6481 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     6541 tcgaccacca agcgaaacat cgcatcgagc aagcacgtac tcggatggaa gccggtcttg
     6601 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     6661 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     6721 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     6781 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6841 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6901 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga agagtatgag
     6961 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7021 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7081 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7141 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7201 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7261 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7321 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7381 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7441 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7501 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7561 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7621 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7681 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7741 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7801 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7861 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgatgatc tcatgaccaa
     7921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8041 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8101 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8161 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8221 gtctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8281 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8341 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8401 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8461 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8521 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8581 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8641 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8701 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
