LOCUS       sequence-325959-        6653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length mouse ADAP1 with N terminal fluorescent tag for 
            purification from insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Duellberg C, Auer A, Canigova N, Loibl K, Loose M
  TITLE     In vitro reconstitution reveals phosphoinositides as cargo-release 
            factors and activators of the ARF6 GAP ADAP1.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2010054118. doi: 
            10.1073/pnas.2010054118. Epub 2020 Dec 18.
  PUBMED    33443153
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2010054118. doi"; pages: " 10.1073/pnas.2010054118. Epub 2020 Dec 
            18"
COMMENT     Sequence Label: mWasabi-ADAP1
FEATURES             Location/Qualifiers
     source          1..6653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..166
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    195..329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(219..238)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     273..292
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2370..2390)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(2412..2429)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(2496..2587)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2554..2571)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(2939..2944,2945..2961,2962..2967)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3137..3670
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  3737..3961
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(4095..4112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4266..4854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4346..4365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5025..5816,5817..5885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5648..5667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5886..5990)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6016..6470)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6153..6174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6364..6383
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat
       61 ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa
      121 ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttcgga tctcctaggc
      181 gatatcagtg atcagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
      241 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      301 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      361 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      421 tggctgatta tgatcctcta gtacttctcg acaagcttgg taccgcatgc ctcgagactg
      481 caggctctag attcgaaagc ggccgcttag ggcttgtgtt tgaaatgggc ttccactgcg
      541 tattcctggg ggagcatagg tctgtccacc accttctgga aggcagcgat ccactccctc
      601 tggtcagatt cagtctcgca ggtgaacagg aacttacggt ctggagtgac gatggtgatg
      661 ccgtggggcc agtggtgacc ctgagtgctc agaggcagac cctccagcac ggtgtatcct
      721 gattccttag agccgatgaa gacctctccg cgggcgaaag cgtccagagg gtccttgaag
      781 tacatcagac ggcggtcgtc catagtgaac caacgcttgc ggaatccttc agtctgcttt
      841 gggccggtct tttccatgta accctccttc aggtagtttc ttgacagctt gggcaccagg
      901 tcggcgtcag aagctccagg gaaggcgacc tgcaggtagt ggaatctggc agccctcaga
      961 gcgttgaacc agtccacgat ttccttgccg tcctcgtggt agatgaagat gttcctagtg
     1021 gagttgtcct tcaggtaggt gacctgcagt ccgtgagggt ggcccatctt ggctggctgg
     1081 aaggtagcgt tcaggtgctc gatcttcatc acggccttgg gttccttagc gtcgttcttg
     1141 ttgaagtact tcagggcgcc ttcgcgctca gtcaggacga actttctgga caggaactgt
     1201 ccgttgtctc ttcccctctt ccacagcagg ccctcacggt aaccggttga gtatggttcc
     1261 tgcttctcga cgtgcacgaa ttcctgcctc tcgtacttag cacggatcca ctgttcgcgc
     1321 agcagctggc agtcagagaa agtggggcgg tagtagaagg gaggcacctt gctttcgaag
     1381 gtggctctgg cagcctcgtt accgtgggaa gccatgaatt cgacctgagc ctcgtcccaa
     1441 gcgtccagcc tcacgctctt gaccttggac acctgaggga tgttacggtg gataccgctg
     1501 caggacaggc agatgaagac tcccagggtg tatgaagccc aatcgggatc gggagcaccg
     1561 caatcggcac agcgagtgtt tcctggtctg gtcaagagtt ccaagagagc acgacgcctt
     1621 tcacctgcca tagaattccc acctccgcca cccttgtaca gttcgtccat gccgtcagta
     1681 gagtttctgg cgacagcgat ctcgtacacg gtgaccttgt tgtagtcctt gtcgtggttc
     1741 aggatttcga tcctgtggtc cacgaagtgg tagtcgggca gcttgacggc cttcttagct
     1801 ctgtagatag tcttgaagtc caccctgtgg tgtccgccac cctccagcag cagcttcatc
     1861 ttgacgtcac ccttcagcac accgtctctg acgtacatcc tctcggtgct agcgtcccag
     1921 ccagtggttt ccttctgcat cacaggaccg ttgggaggga agttttctcc cttcaggtgg
     1981 atctcgtaga tgaaggagtc ttccttcatg ctgatgtcgg acttcacctt gacgattccc
     2041 ttgtcctcga aagtcatggt tctctcccaa gagtagcctt ctgggaagct ctgcttgaag
     2101 tagttgggga tgtcgtcagg gtacttggtg aaagccctgt taccgtagct gaaggcagtg
     2161 gtcaggatgt cgtaggagaa aggcagtgga gcaccctcct tcacttccag gttgatagtg
     2221 ttggttccgt cgtaaggctt gccctctcct tctccctcga tgacgaaggc gtgtccgttg
     2281 acgtttcctt ccatcttcag cttgattttc atatcgggct taataactcc cattgtcgtt
     2341 tcttcacctt tgctaaccat tcccatggcg ccctgaaaat acaggttttc ggtcgttggg
     2401 atatcgtaat cgtgatggtg atggtgatgg tagtacgaca tggtttcgga ccgagatccg
     2461 cgcccgatgg tgggacggta tgaataatcc ggaatattta taggtttttt tattacaaaa
     2521 ctgttacgaa aacagtaaaa tacttattta tttgcgagat ggttatcatt ttaattatct
     2581 ccatgatcta ttaatattcc ggagtatacg tagccaacca ctagaactat agctagagtc
     2641 ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg
     2701 ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg
     2761 cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg
     2821 cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct
     2881 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtt
     2941 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     3001 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3061 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3121 cgtgggtcga tgtttgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
     3181 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
     3241 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
     3301 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
     3361 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
     3421 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
     3481 cgagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
     3541 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
     3601 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     3661 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
     3721 attgtcacaa cgccgcgaat atagtcttta ccatgccctt ggccacgccc ctctttaata
     3781 cgacgggcaa tttgcacttc agaaaatgaa gagtttgctt tagccataac aaaagtccag
     3841 tatgcttttt cacagcataa ctggactgat ttcagtttac aactattctg tctagtttaa
     3901 gactttattg tcatagttta gatctatttt gttcagttta agactttatt gtccgcccac
     3961 acccgcttac gcagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc
     4021 ggctggtcta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     4081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6001 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     6061 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     6121 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     6181 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     6241 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     6301 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     6361 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     6421 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     6481 attcaggctg caaataagcg ttgatattca gtcaattaca aacattaata acgaagagat
     6541 gacagaaaaa ttttcattct gtgacagaga aaaagtagcc gaagatgacg gtttgtcaca
     6601 tggagttggc aggatgtttg attaaaaaca taacaggaag aaaaatgccc cgc
//
