LOCUS       sequence-32596-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin 
            JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM
  TITLE     Modification of superoxide dismutase 1 (SOD1) properties by a GFP 
            tag--implications for research into amyotrophic lateral sclerosis 
            (ALS).
  JOURNAL   PLoS One. 2010 . 5(3):e9541.
  PUBMED    20221404
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010 . 5(3):e9541."
COMMENT     Sequence Label: pF155 pcDNA3.1(+)SOD1G93A
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(635..652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    641..752
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg
      121 gtggaattcc atggcgacga aggccgtgtg cgtgctgaag ggcgacggcc cagtgcaggg
      181 catcatcaat ttcgagcaga aggaaagtaa tggaccagtg aaggtgtggg gaagcattaa
      241 aggactgact gaaggcctgc atggattcca tgttcatgag tttggagata atacagcagg
      301 ctgtaccagt gcaggtcctc actttaatcc tctatccaga aaacacggtg ggccaaagga
      361 tgaagagagg catgttggag acttgggcaa tgtgactgct gacaaagatg ctgtggccga
      421 tgtgtctatt gaagattctg tgatctcact ctcaggagac cattgcatca ttggccgcac
      481 actggtggtc catgaaaaag cagatgactt gggcaaaggt ggaaatgaag aaagtacaaa
      541 gacaggaaac gctggaagtc gtttggcttg tggtgtaatt gggatcgccc aataacgctc
      601 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      661 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      721 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtangtg tcattctatt
      781 ctggggggtg gggtggggca ggacagcaag gggga
//
