LOCUS       sequence-325960-        5937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length mouse ADAP1 for purification from insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Duellberg C, Auer A, Canigova N, Loibl K, Loose M
  TITLE     In vitro reconstitution reveals phosphoinositides as cargo-release 
            factors and activators of the ARF6 GAP ADAP1.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2010054118. doi: 
            10.1073/pnas.2010054118. Epub 2020 Dec 18.
  PUBMED    33443153
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2010054118. doi"; pages: " 10.1073/pnas.2010054118. Epub 2020 Dec 
            18"
COMMENT     Sequence Label: ADAP1
FEATURES             Location/Qualifiers
     source          1..5937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     297..318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        481..585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(586..654,655..1446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(804..823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1617..2205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2106..2125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2359..2376
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2510..2734
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2801..3334)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3523..3528,3529..3545,3546..3551))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3903..3994
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3919..3936
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4061..4078
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4100..4120
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5426..5560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5463..5482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5517..5536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5589..5754
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg
      301 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      361 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      421 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
      481 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      541 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      601 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      661 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      721 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      781 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      841 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      901 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      961 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1021 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1081 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1141 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1201 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1261 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1321 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1381 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1441 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1741 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2281 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2341 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2401 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg
     2461 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     2521 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     2581 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     2641 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     2701 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     2761 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     2821 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     2881 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     2941 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     3001 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     3061 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     3121 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     3181 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     3241 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     3301 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     3361 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     3421 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     3481 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     3541 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     3601 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     3661 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     3721 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     3781 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     3841 ttcgcccagg actctagcta tagttctagt ggttggctac gtatactccg gaatattaat
     3901 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
     3961 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
     4021 catcgggcgc ggatctcggt ccgaaaccat gtcgtactac catcaccatc accatcacga
     4081 ttacgatatc ccaacgaccg aaaacctgta ttttcagggc gccatggcag gtgaaaggcg
     4141 tcgtgctctc ttggaactct tgaccagacc aggaaacact cgctgtgccg attgcggtgc
     4201 tcccgatccc gattgggctt catacaccct gggagtcttc atctgcctgt cctgcagcgg
     4261 tatccaccgt aacatccctc aggtgtccaa ggtcaagagc gtgaggctgg acgcttggga
     4321 cgaggctcag gtcgaattca tggcttccca cggtaacgag gctgccagag ccaccttcga
     4381 aagcaaggtg cctcccttct actaccgccc cactttctct gactgccagc tgctgcgcga
     4441 acagtggatc cgtgctaagt acgagaggca ggaattcgtg cacgtcgaga agcaggaacc
     4501 atactcaacc ggttaccgtg agggcctgct gtggaagagg ggaagagaca acggacagtt
     4561 cctgtccaga aagttcgtcc tgactgagcg cgaaggcgcc ctgaagtact tcaacaagaa
     4621 cgacgctaag gaacccaagg ccgtgatgaa gatcgagcac ctgaacgcta ccttccagcc
     4681 agccaagatg ggccaccctc acggactgca ggtcacctac ctgaaggaca actccactag
     4741 gaacatcttc atctaccacg aggacggcaa ggaaatcgtg gactggttca acgctctgag
     4801 ggctgccaga ttccactacc tgcaggtcgc cttccctgga gcttctgacg ccgacctggt
     4861 gcccaagctg tcaagaaact acctgaagga gggttacatg gaaaagaccg gcccaaagca
     4921 gactgaagga ttccgcaagc gttggttcac tatggacgac cgccgtctga tgtacttcaa
     4981 ggaccctctg gacgctttcg cccgcggaga ggtcttcatc ggctctaagg aatcaggata
     5041 caccgtgctg gagggtctgc ctctgagcac tcagggtcac cactggcccc acggcatcac
     5101 catcgtcact ccagaccgta agttcctgtt cacctgcgag actgaatctg accagaggga
     5161 gtggatcgct gccttccaga aggtggtgga cagacctatg ctcccccagg aatacgcagt
     5221 ggaagcccat ttcaaacaca agccctaagc ggccgctttc gaatctagag cctgcagtct
     5281 cgaggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     5341 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5401 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     5581 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     5641 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     5701 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
     5761 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
     5821 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
     5881 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatggg
//
