LOCUS       sequence-325967-       12936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FL human talin2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard
            T
  TITLE     A distinct talin2 structure directs isoform specificity in cell 
            adhesion.
  JOURNAL   J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 
            10.1074/jbc.RA119.010789. Epub 2020 Jun 30.
  PUBMED    32605925
REFERENCE   2  (bases 1 to 12936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010789"; journalName: "J Biol Chem"; date: 
            "2020-09-11- 11"; volume: "295"; issue: "37"; pages: "12885-12899"
COMMENT     Sequence Label: pET28_hTLN2-FL
FEATURES             Location/Qualifiers
     source          1..12936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..1083)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1045..1064
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1084..1161)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1367..1386
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1470..1489
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1470..1488
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1489..1513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1528..1550
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1569..1592
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1593..1616
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9270..9287
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9340..9358)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9354..9401
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9438..9893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9525..9544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9735..9756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9985..10800)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9985..10800)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10708..10727
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10792..10811
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10922..11510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11411..11430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11664..11681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11696..11835
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11761..11780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11921..11943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11937..12128)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     1201 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     1261 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     1321 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     1381 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     1441 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
     1501 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg
     1561 gcagcagcca tcatcatcat catcaccatc atctggaagt tctgttccag gggccccata
     1621 tggtggccct gtcctttaag atttgtgtgc gccactgcaa cgtggtgaag accatgcagt
     1681 ttgaaccatc tacagctgtg tacgatgcgt gtcgagtcat tcgggaacgg gtgcctgagg
     1741 cacaagctgg gcaagcttct gactatggac tctttctttc ggatgaagac ccgaggaaag
     1801 ggatttggct ggaagcgggc agaacactgg attactacat gttgcggaat ggggatattt
     1861 tggaatataa aaagaaacag agacctcaga aaatccggat gctggatgga tctgtgaaga
     1921 cagtgatggt ggatgattcc aagactgtgg gggagctcct ggtcactatt tgtagcagaa
     1981 taggaataac aaattatgaa gaatactcct taatccaaga aactatcgaa gaaaagaaag
     2041 aggaaggaac gggcacactc aaaaaagaca ggacactgtt acgagatgag aggaaaatgg
     2101 agaagttgaa ggccaagctg cacacagatg atgacctaaa ttggctggat cacagccgaa
     2161 cattcagaga acaaggagta gatgaaaacg aaacgttgct gcttagacgg aagttctttt
     2221 actctgatca gaatgtagat tcgagagacc ccgtgcagct gaacttgctt tatgttcagg
     2281 cacaggatga catcctgaat ggctctcacc ctgtctcctt cgagaaagct tgcgagtttg
     2341 gtggatttca agcccagata caatttggac ctcatgtgga acataaacac aaacctggat
     2401 ttttagatct gaaggaattc ctgcccaaag aatacatcaa gcagagagga gctgaaaaga
     2461 ggatctttca ggagcataag aactgcggag agatgagcga gatagaagcc aaggtcaagt
     2521 acgtcaaact cgcacggtcc ctccgcacat atggcgtgtc cttcttcctg gtgaaggaga
     2581 agatgaaagg caagaacaag ctggtgcctc gcctgctggg gatcaccaaa gactcggtga
     2641 tgcgcgtgga tgagaagacc aaggaagtgc tgcaggagtg gcccctcacc accgtcaagc
     2701 gctgggcagc ctcacccaag agcttcacac tggattttgg ggagtatcag gaaagctact
     2761 attcagtaca aaccaccgag ggagagcaga tatcccagct gattgcaggc tacattgaca
     2821 tcatcctgaa aaagaaacaa agtaaagatc gatttggact agaaggtgat gaggagtcaa
     2881 ccatgttaga agagtccgtt tccccaaaaa agtccaccat cttgcagcag cagttcaacc
     2941 ggaccgggaa ggcagagcac ggctcagtgg cgctgccggc cgtgatgcgc tcgggctcca
     3001 gcgggcctga gaccttcaac gttggcagca tgccctcgcc acagcagcag gtcatggttg
     3061 ggcagatgca ccgaggccac atgccgccac tgacctcagc ccagcaggcc ctgatgggga
     3121 ccatcaacac aagcatgcac gccgtccagc aggcccagga tgatctcagt gagctcgact
     3181 cgctgccacc tctcggccag gatatggcat ctagggtatg ggttcagaac aaagtcgacg
     3241 aatccaaaca cggaatccat tctcaagttg atgctatcac ggccggaacg gcttcagttg
     3301 ttaacctcac agctggtgac cctgcagaca ctgactacac agctgtggga tgtgcgatca
     3361 ccactatttc ttccaacccg acggagatgt ccaagggtgt gaagctattg gccgccctca
     3421 tggatgatga ggtgggcagc ggggaggact tgctcagagc tgccaggacc ctcgctgggg
     3481 cggtgtcaga cttgctgaaa gctgtgcagc ctacttctgg agagcctcga cagacagttt
     3541 tgactgctgc tggcagcatc ggacaagcca gtggggatct tctgagacag attggagaga
     3601 atgagactga tgagcgattc caggatgttt taatgagttt ggccaaagct gttgccaatg
     3661 cagctgccat gttggtacta aaggcaaaga atgttgccca agtggccgaa gacactgtcc
     3721 tacagaacag ggtaattgct gctgccaccc agtgtgccct ctccacctcc cagcttgtgg
     3781 catgtgccaa ggttgtgagc cccactatta gctcccctgt gtgccaggag cagctgattg
     3841 aagcagggaa gctggtggac cgctcggtgg agaactgtgt ccgtgcctgc caggcggcca
     3901 ctaccgatag tgagctcctg aagcaggtca gcgcagcggc cagcgtggtc agccaggccc
     3961 tccatgatct cctgcagcat gtgcggcagt ttgccagccg aggcgagccc atcggccgct
     4021 acgaccaggc tactgacacc atcatgtgtg tcaccgagag catcttcagc tccatgggtg
     4081 acgctggtga aatggtgcgc caggcgcggg ttctggccca agccacatca gacctcgtca
     4141 atgccatgag gtcagatgca gaagccgaaa tcgacatgga gaattcaaag aagctcctgg
     4201 cagcagcaaa actcttagct gactccactg ctcgcatggt ggaagctgca gagggggctg
     4261 cagccaaccc agagaatgag gaccagcagc aaaggctgag agaagctgca gaaggcctcc
     4321 gggtagcaac caacgcagct gcccagaatg ctattaagaa aaaaattgtc aaccgactgg
     4381 aggttgcagc caagcaggcc gcagcggcag ctacacagac catcgccgcc tcccagaatg
     4441 cagctgtttc caacaagaac cctgcggccc agcagcagct ggtccagagt tgcaaggcag
     4501 tggctgatca catccctcag ctggtccagg gagtgagggg gagccaagct caagctgaag
     4561 acctgagtgc ccagctggct ctcatcatct ccagccagaa cttcctccag cctggaagca
     4621 agatggtgtc ctctgccaaa gccgcagtgc ccaccgtgag tgaccaggcc gcagccatgc
     4681 agctgagcca gtgtgccaag aacctggcca ccagcttggc ggagctgcgt accgcctcgc
     4741 agaaggccca tgaagcttgt ggtccgatgg aaatcgattc agctctgaat acggtgcaga
     4801 cgcttaagaa tgaactgcag gatgccaaga tggcagccgt ggagagccag ctgaagccac
     4861 ttccagggga aacgctggaa aaatgtgctc aggacctggg aagcacatcc aaggcggtgg
     4921 gctcctccat ggcacagctg ctgacctgtg ctgctcaagg caacgaacac tacacagggg
     4981 tggctgctag agagacggcc caagctctga aaacactggc ccaggccgcc cgtggagtgg
     5041 ctgcatcgac aaccgacccc gcggccgccc atgccatgtt agattctgct cgagacgtga
     5101 tggagggctc cgccatgctc attcaagagg ccaagcaggc cctgattgca cctggagatg
     5161 cagagcgtca acaaagactg gctcaggtgg ctaaagccgt ctcacactcc ttgaataact
     5221 gcgtaaattg cctccctggg cagaaggatg tggacgtggc cttgaagagc atcggggagt
     5281 ccagcaagaa gctgcttgtg gattcgctac ctccaagcac gaagcctttc caggaagccc
     5341 agagtgaact gaaccaggca gcagctgatc tgaaccagtc tgctggggaa gtggtccatg
     5401 ccacccgggg ccagagtgga gagttggctg cagcctctgg aaagttcagt gatgattttg
     5461 atgaattcct cgatgctggc attgagatgg ctggccaagc tcagacaaaa gaagaccaga
     5521 tccaagtgat agggaacctc aagaatatct cgatggcatc cagcaagctg ctgttagctg
     5581 ccaagtctct ctctgtagat ccaggagctc ccaatgcgaa aaatctcctg gctgcagctg
     5641 caagagctgt gacagagagc atcaatcaac tcatcactct gtgtacccaa caagctccgg
     5701 gccagaaaga gtgcgataat gccctgcggg agctcgagac tgtgaagggg atgttggaca
     5761 atcctaatga acctgttagt gacctctctt actttgactg cattgagagt gtgatggaaa
     5821 actccaaggt tctgggtgaa tcgatggcag ggattccaca gaatgccaag accggagacc
     5881 tccctgcctt tggggaatgt gtggggattg catccaaggc tctctgtggg ctgacagagg
     5941 ctgcagccca ggctgcatac ttggttggca tctctgatcc aaacagccag gcaggccacc
     6001 agggcctggt ggaccccatc cagtttgcca gggctaacca ggccatccag atggcatgcc
     6061 agaacttggt ggaccctggc agcagcccat cacaggtcct gtcagccgcc acaattgttg
     6121 ccaagcacac gtcagccttg tgtaatgcct gccgcatcgc ctcatccaag acggccaacc
     6181 cagtagccaa gaggcacttc gtccagtcag ccaaggaagt cgccaacagc actgccaacc
     6241 tggtgaagac catcaaggcc ctggatgggg atttctctga agacaaccgc aataagtgtc
     6301 gcatcgccac cgcacccttg attgaagctg tggagaacct gacagcgttc gcctcaaacc
     6361 ctgagtttgt cagcattcct gcccagatca gctccgaggg ttcccaggca caggaaccaa
     6421 tcctggtctc agccaagacc atgctggaga gttcatcgta cctcattcgc actgcgcgct
     6481 ctctggccat caaccccaaa gacccaccca cctggtctgt actggctgga cattcccata
     6541 cagtgtccga ctccatcaag agtctcatca cttctatcag ggacaaggcc cctggacaga
     6601 gggagtgtga ttactccatc gatggcatca accggtgcat ccgggacatc gagcaggcct
     6661 cgctggccgc cgtcagccag agcctggcca cgagggacga catctctgtg gaggccctgc
     6721 aggagcagct gacttcggtg gtccaggaaa tcggacacct tatcgatccc atcgccacag
     6781 cggctcgggg agaagcagct cagctgggac ataaggtgac acaactggca agctattttg
     6841 agcccttgat cttagccgca gttggtgtgg cctccaagat tcttgatcat cagcagcaga
     6901 tgacggtgct ggaccagacc aagactctcg cagagtctgc cttgcagatg ttgtatgcag
     6961 ccaaagaagg tggcggaaac cccaaggcac aacacaccca tgacgccatc acagaggccg
     7021 cccagttgat gaaggaagcc gtggatgaca tcatggtgac gctgaacgaa gctgccagtg
     7081 aagtggggct ggttgggggc atggtggacg ccattgcaga agccatgagc aagctggatg
     7141 aaggcactcc tccagaacca aagggaacat ttgtcgacta tcagacgact gtggttaaat
     7201 actccaaagc cattgcggtg acagctcagg aaatgatgac taagtcggtt actaacccgg
     7261 aggagttggg aggactggct tcacaaatga ccagtgacta tgggcacctg gctttccagg
     7321 gccagatggc agcagccacg gcggaaccag aggagatcgg attccagatt cgcactcgtg
     7381 tgcaggacct gggccacggc tgtatcttcc tggtgcagaa ggcaggggcc ctccaggtct
     7441 gccccacaga cagctacacc aagagggagc tgatcgaatg cgcccgtgcc gtcacggaaa
     7501 aggtctcctt ggtgctctcg gctctccagg ccgggaacaa aggaacccag gcatgcatta
     7561 cagccgccac cgctgtgtct gggatcattg ccgacctgga caccaccatt atgtttgcaa
     7621 cagcggggac gctgaatgca gagaacagtg agaccttcgc agaccacagg gagaacattc
     7681 tcaagacggc caaggccttg gtagaagaca cgaaactact tgtgtcagga gctgcgtcca
     7741 ctcctgacaa gctggcccag gcggcccagt cctcagcagc caccatcacc cagctcgcag
     7801 aagtggtcaa gctgggggca gccagcctgg gctccgacga ccccgagacc caggtggttt
     7861 tgatcaatgc catcaaagat gtggccaagg ccctttctga tctcatcagt gctaccaagg
     7921 gagctgccag caagccagtg gacgaccctt ccatgtacca gctcaagggg gctgccaagg
     7981 tgatggtgac caatgtcacc tcgctcctca agactgtaaa ggcagtggag gatgaggcca
     8041 cccggggcac cagggcgctt gaggccacaa ttgaatgcat aaagcaggag cttacggtgt
     8101 tccagtcaaa agacgtacct gaaaagacat catcacctga agaatccata aggatgacga
     8161 aaggcatcac catggcaaca gccaaagccg tggcagctgg gaactcatgt agacaggagg
     8221 acgtgattgc tactgccaac ctgagccgga aagccgtgtc agatatgttg acggcttgca
     8281 agcaagcatc cttccacccc gatgtcagtg acgaggtgag aaccagagcc ttgcgtttcg
     8341 ggacggagtg cacccttggc tacttggacc tcctggagca cgtcttggtg attcttcaga
     8401 aaccaacccc agaactcaag cagcagctgg ccgctttctc caagcgagtc gccggcgctg
     8461 tgacagagct catccaggcg gcggaagcca tgaaaggaac agagtgggtg gatccagaag
     8521 acccaactgt cattgcagaa acagagttac tgggggctgc agcatccatc gaagctgctg
     8581 ctaagaagtt agagcaactg aagccaagag caaaaccaaa acaagcggat gagaccctgg
     8641 actttgagga acagatcttg gaagctgcta aatccattgc tgctgccaca agcgccctgg
     8701 tcaaatcggc ctcagcagcc cagagggagc tggtggccca aggaaaggtg ggctccatcc
     8761 ctgccaacgc tgcagacgac ggacagtggt cacaagggct gatttctgct gcccggatgg
     8821 tggcggctgc gaccagcagt ctctgtgagg cggccaatgc ctccgttcag ggacacgcca
     8881 gcgaggagaa gctcatctca tctgccaagc aggtcgccgc ttccacggct cagctgctgg
     8941 tggcctgcaa ggtgaaggcc gaccaggatt cagaggccat gaggcggcta caggcggcag
     9001 gaaatgctgt gaaaagagcc tcagacaatc ttgtccgtgc agcccagaag gcagcatttg
     9061 gcaaagctga tgacgacgat gttgtagtga aaaccaagtt tgtggggggc attgctcaga
     9121 tcatcgccgc ccaggaagaa atgctaaaga aagagcgaga actggaagaa gcaaggagaa
     9181 aactggccca aatccgccag cagcagtata agtttttacc caccgagctg agggaagatg
     9241 agggctgaaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
     9301 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     9361 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     9421 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     9781 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     9841 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     9901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9961 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa
    10021 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
    10081 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
    10141 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
    10201 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
    10261 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
    10321 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
    10381 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
    10441 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
    10501 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
    10561 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
    10621 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
    10681 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
    10741 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat
    10801 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc
    10861 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10921 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10981 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11041 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    11101 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11161 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11221 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11281 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11341 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11401 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11461 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11521 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11581 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    11641 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
    11701 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
    11761 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
    11821 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    11881 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    11941 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
    12001 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
    12061 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
    12121 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
    12181 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
    12241 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
    12301 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
    12361 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
    12421 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
    12481 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
    12541 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc
    12601 gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg
    12661 aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc
    12721 gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct
    12781 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
    12841 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
    12901 taatgagtga gctaacttac attaattgcg ttgcgc
//
