LOCUS       sequence-325968-        6548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Motile fragment of mouse KIF13B (aa 1-584) for purification from 
            insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Duellberg C, Auer A, Canigova N, Loibl K, Loose M
  TITLE     In vitro reconstitution reveals phosphoinositides as cargo-release 
            factors and activators of the ARF6 GAP ADAP1.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2010054118. doi: 
            10.1073/pnas.2010054118. Epub 2020 Dec 18.
  PUBMED    33443153
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2010054118. doi"; pages: " 10.1073/pnas.2010054118. Epub 2020 Dec 
            18"
COMMENT     Sequence Label: KIF13B 1-584
FEATURES             Location/Qualifiers
     source          1..6548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  58..282
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(349..882)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1052..1057,1058..1074,1075..1080))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1432..1523
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1448..1465
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1590..1607
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1629..1649
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3585..3719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3622..3641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3676..3695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3748..3913
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4097..4551
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4184..4203)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4393..4414
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4577..4681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4682..4750,4751..5542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4900..4919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5713..6301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6202..6221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6455..6472
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtaacctggc aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt
       61 gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt
      121 aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact
      181 ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta
      241 ttaaagaggg gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt
      301 accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt
      361 acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc
      421 cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt
      481 ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc
      541 ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac
      601 gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt
      661 cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc
      721 tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc
      781 agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg
      841 gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcaaacatc gacccacggc
      901 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
      961 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     1021 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
     1081 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
     1141 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
     1201 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
     1261 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
     1321 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga
     1381 ctctagctat agttctagtg gttggctacg tatactccgg aatattaata gatcatggag
     1441 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
     1501 ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
     1561 gatctcggtc cgaaaccatg tcgtactacc atcaccatca ccatcacgat tacgatatcc
     1621 caacgaccga aaacctgtat tttcagggcg ccatgggaga tagcaaagtg aaagtggccg
     1681 ttagagttag accgatgaac cgtagagaga ttgacctgca tacaaagtgt gtcgttgatg
     1741 tggaggctaa caaggtcatc ctgaaccctg tgaacaccaa cctgagcaag ggagacgctc
     1801 gcggtcagcc caagatcttc gcctacgacc actgcttctg gtccatggac gagagcgtgc
     1861 gtgaaaagta cgctggtcag gaagacgtct tcaagtgcct gggcgagaac atcctgcaga
     1921 acgccttcga cggatacaac gcttgcatct tcgcctacgg tcagaccggt tctggcaagt
     1981 catacactat gatgggtacc gctgaccagc ctggcctgat cccaaggctg tgctccggtc
     2041 tgttcgagag aactcagaag gaggaaaacg aggaacagtc tttcaaggtc gaagtgtcat
     2101 acatggagat ctacaacgaa aaggtgcgcg acctgctgga cccaaagggc tccaggcaga
     2161 ccctgaaggt cagagagcac agcgtgctgg gtccatacgt cgacggcctg tccaagctgg
     2221 ctgtgactag ctacaaggac atcgaatccc tgatgagcga gggtaacaag tctcgcactg
     2281 tcgctgccac caacatgaac gaggaatcca gccgttcaca cgccgtgttc aagatcaccc
     2341 tgactcacac cctgtacgac gtcaagtctg gcacctcagg agaaaaagtg ggcaagctgt
     2401 ccctggtcga cctggctgga agcgagagag ctactaagac cggtgctgct ggcgaccgtc
     2461 tgaaggaagg atcaaacatc aacaagtccc tgaccactct gggtctggtc atctccgctc
     2521 tggccgacca gggagctggc aagaacaaga acaagttcgt gccctaccgt gacagcgtcc
     2581 tgacttggct gctgaaggac agcctgggtg gcaactctaa gactgctatg gtggccaccg
     2641 tctccccagc tgccgacaac tacgacgaga ctctgagcac cctgcgttac gctgacaggg
     2701 ccaagcacat cgtgaaccac gctgtggtca acgaagaccc taacgccagg atcatccgcg
     2761 acctgcgcga ggaagtcgag aagctgaggg aacagctgac caaggctgaa gccatgaagt
     2821 ctcccgagct gaaggacaga ctggaggaat cagaaaagct gatccaggag atgactgtga
     2881 cctgggagga aaagctgagg aagaccgagg aaatcgctca ggaaagacag aagcagctgg
     2941 agtctctggg aatctcactg cagacttccg gcatcaaggt cggagacgac aagtgcttcc
     3001 tggtcaacct gaacgctgac ccagccctga acgaactgct ggtgtactac ctgaaggagc
     3061 acaccctgat cggttccgct aacagccagg acatccagct gtgcggcatg ggaatcctgc
     3121 ctgaacacgg tatcatcgac atcactccag agggacaggt ggtcctgacc ccacagaaga
     3181 acactcgcac cttcgtgaac ggttcttcag tctccagccc catccagctg caccacggag
     3241 acaggatcct gtggggtaac aaccacttct tcagactgaa cctgcctaag aagaagaaga
     3301 aggctgagcg cgaagacgag gaccgtgaag cctctctgaa gaacgactct tcatccgagc
     3361 agctggacgc tgacggtgac agctcttcag aggtgtccag cgaataagcg gccgctttcg
     3421 aatctagagc ctgcagtctc gaggcatgcg gtaccaagct tgtcgagaag tactagagga
     3481 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3541 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     3601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     3721 gatcactgat atcgcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt
     3781 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
     3841 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
     3901 ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca
     3961 actccatgtg acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt
     4021 tctgtcatct cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc
     4081 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4141 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4201 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4261 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4321 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4381 acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4501 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     4561 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5041 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5641 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5701 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5761 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5821 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5881 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5941 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6001 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6061 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6481 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     6541 ccagccgc
//
