LOCUS       sequence-32597-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin 
            JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM
  TITLE     Modification of superoxide dismutase 1 (SOD1) properties by a GFP 
            tag--implications for research into amyotrophic lateral sclerosis 
            (ALS).
  JOURNAL   PLoS One. 2010 . 5(3):e9541.
  PUBMED    20221404
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010 . 5(3):e9541."
COMMENT     Sequence Label: pF141 pAcGFP1 SOD1WT
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..751
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg ccgagctgtt
       61 caccggcatc gtgcccatcc tgatcgagct gaatggcgat gtgaatggcc acaagttcag
      121 cgtgagcggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcctg tgccctggcc caccctggtg accaccctga gctacggcgt
      241 gcagtgcttc tcacgctacc ccgatcacat gaagcagcac gacttcttca agagcgccat
      301 gcctgagggc tacatccagg agcgcaccat cttcttcgag gatgacggca actacaagtc
      361 gcgcgccgag gtgaagttcg agggcgatac cctggtgaat cgcatcgagc tgaccggcac
      421 cgatttcaag gaggatggca acatcctggg caataagatg gagtacaact acaacgccca
      481 caatgtgtac atcatgaccg acaaggccaa gaatggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggatggca gcgtgcagct ggccgaccac taccagcaga atacccccat
      601 cggcgatggc cctgtgctgc tgcccgataa ccactacctg tccacccaga gcgccctgtc
      661 caaggacccc aacgagaagc gcgatcacat gatctacttc ggcttcgtga ccgccgccgc
      721 catcacccac ggcatggatg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattccatg gcgacgaagg ccgtgtgcgt gctgaagggc gacggcccag tgcagggcat
      841 catcaatttc gagcagaagg aaagtaatgg accagtgaag gtgtggggaa gcattaaagg
      901 actgactgaa ggcctgcatg gattccatg
//
