LOCUS       sequence-325985-       13124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for the diffuse visualization of H2O2 production in cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Enyedi B, Kala S, Nikolich-Zugich T, Niethammer P
  TITLE     Tissue damage detection by osmotic surveillance.
  JOURNAL   Nat Cell Biol. 2013 Sep;15(9):1123-30. doi: 10.1038/ncb2818. Epub 
            2013 Aug 11.
  PUBMED    23934216
REFERENCE   2  (bases 1 to 13124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2818"; 
            journalName: "Nat Cell Biol"; date: "2013-09"; volume: "15"; issue: 
            "9"; pages: "1123-30"
COMMENT     Sequence Label: LR (bact: HyPer)
FEATURES             Location/Qualifiers
     source          1..13124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    41..61
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5368..5392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5395..6831
                     /codon_start=1
                     /product="improved fluorescent indicator for intracellular 
                     H2O2 (Bilan et al., 2013)"
                     /label=HyPer-3
                     /translation="MEMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMY
                     LHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP
                     MADLAGEKLLMLEDGHCLRDQAMSAGYNSDNVYIMADKQKNGIKANFKIRHNVEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSFQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDEL
                     YNVDGGSGGTGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICT
                     TGKLPVPWPTLVTTLGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKT
                     RAEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNGTGFCFEAGADEDTHFRATSLET
                     LRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQL
                     AEAIRARMDGHFDKVLKQAV"
     protein_bind    complement(6833..6857)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6859..6993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7056..7076)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7084..7100)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7116..7250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7552..8265,8266..8268,8269..8271))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7555..8265,8266..8268,8269..8271))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(9494..9681,9682..9693)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9727..9743)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9727..9743)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9780..9800)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9780..9798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9819..9835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9832..9854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9843..9859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9867..9873,9874..9891,9892..9897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9912..9933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10050..10067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10221..10809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10301..10320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10980..11771,11772..11840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11603..11622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11841..11945)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12108..12129)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(12582..12593,12594..12756)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 cgagcaggaa acagctatga ccatgattac gccaagctat caactttgta tagaaaagtt
       61 gaaaattcca gtttgaagaa acttttcaag actcaccttt tcactgttgg tcttaattta
      121 ggttgattat ttttgtcaat ttaataatta cattgtattg ttcatatttt attgtcttat
      181 tgtggtgttt ttacagcttt tttattgttc tgctttgtta ctgtttgcct tgtatgcatt
      241 gtagcgcttt gggtctgaga aaagcgcact ataaataaac tgtattatta ttattaggga
      301 tggacatcac ggtggcgcga tgggtggcac aattacctca cagcaagtag gttgctgttt
      361 agagccccgg ctggatcagt tggcagttct gtgtggagtt tgcatgttct ccctgttcaa
      421 gtaggtttcc tcccggtgct ctggtttccc ctcacaagtc caaagacatg atgtataggt
      481 gaatggggta agctaaattg accatagtga gtgtgtgtgt gtgtgtgaat gagagtgtat
      541 aggtgtttcc cagtgttagg ttgcaactgg aaggacatct gctgtgtaaa acatatgcag
      601 gataagttgg cggttcattc ccctatggtg acccctggtt aattaaagga ctaagttgaa
      661 aatgaatgaa tgaatgaatg attagagata aaccgatacc acttttttac aaaccgatat
      721 gagtacaagt ataatagttt ctgttactcg acaatactga gtaccgattc tgatacttct
      781 tggatttggt ttcaatgtaa ttaatttgaa agaaggtttt atttcttgcc tgtcgcaagg
      841 atgaacacaa aaatctttac cagaaggata ttttaagcta agaatatacg cacgttgttg
      901 acaaccctgt atcgattacc gattaaacgt ggacatttaa aggataagtt atgcttgcgc
      961 catcacaact ttacacatta catgatcaaa gatcatattg aatattcggt ttattcgtca
     1021 gaagcctata gatttgttaa aagtgtattt tataaacgga tggtttataa agactaagct
     1081 attaatatat cagacattca ttaatacatc aagacatcga tgtcaggtag gacaacaaat
     1141 cacatccatg taatgaaatc ttgttgagtg gtattggtgc gtggtttcag catttcatgt
     1201 gcaatacgag taagaatatt ttaaccaagt atcgacccaa aacctgatac tggtatcggt
     1261 gcatccctta ttattattat tatcaaaaac acacttgttt tatttatgtg ggtgccaaac
     1321 aacaaactat gaaatgcagc atgacacgcc caaaaagtgt tcctttgaga acaacttcat
     1381 aaatatgttt cttatcatta tgcatgaatg gtgtactggg agagctttgg tatcagaatt
     1441 gagataagag actttggcat tctaaaggac aatgacatta tcacactgac agtcaacaac
     1501 actttattgc tctggaaatc ttatgtcatg gggcaaagct gaggttcatt aggacagtac
     1561 cttatttaga gatgaatggg aaggctgtag tgggctgcta tcagggaagg gtggtggtcc
     1621 tcgggggaac tatggactca caaaagcaca tctgctgtaa caagatcccc tgtgcaatct
     1681 acaaaagtta caaagagaag catggttggt taaaaaatag aattgactgg atatgctgaa
     1741 tgcattgttt gttgtgtgtt acatcacatt aaccacagat tggtgctaaa tatttgctta
     1801 tcttataaaa acctgtgtgt ttataaaggg gggtgtggat ggatgcttgg cattctttga
     1861 aaagatgttg ggggatggaa ggaggagacc ttattgatga gtatccagtt tgcagataac
     1921 aagtttttag acattcatac tatgatgctg tttatgtctg ttttttctca ttttttctat
     1981 acacaaacag ctataaaaca ttcactttta tatctgatgc tgtatattaa atttcgttgt
     2041 atttattctg tttgctctgt tgggtcatag ctagggccag acagaatctg ctgacatttt
     2101 ttgctttttc tgcagagaat tttgtaaaca atctgtgatt tatgcgagat tttttcggga
     2161 gtatcgtaac taaaaactta ctatatgaaa taaaaaaaaa taataccttt ttaactttta
     2221 ataaatatat acaatgcaag tccacctaga tccaattatt tggtcgacaa agcaagtctc
     2281 tcacataata tatcttctaa aagacagaaa acattacttt cagaccatat tgtaatcaaa
     2341 tgaacacttt catattagtc aataatatta cggaaatgta tttaaaaact gaataaacat
     2401 atatttacac ctttgtcttt atttctagag tgcttttaca atgtagattg tgtcaaagca
     2461 gcttaactta gaggattagt atagtaaatt gaaactgtgt cagtccattt tttaagagtt
     2521 taagttcagt tcagtttagt tcagtgtgag ttaattttta ctgctgagag tccaaacact
     2581 aaagagcaaa tccaaataca caagtcaata aataaactca atgatgggct aaagatctgg
     2641 ggaaatctgc agatttctgt gcgcacagat tctgtgtggg cctagtcatt acctatactc
     2701 aacgtttact ctgagaaact tccatatttt agaggtaaaa tgctcagaat tgtaaaaagt
     2761 cttgtcaaaa tacttggaat ttgccaaaaa caaaataaaa gtcacatcac ctcattgtcc
     2821 ttggtatcag caagcacttt taacacaatc agctgttttt gtttataact cccactgaag
     2881 gccatgtgca ttactcccaa gttacacagt aaaaagtcac atgtagttta tcatgatcac
     2941 tgctgctcaa gcacagcttt atctgttatt cagtgtggca atatctgaaa tgtgtgcaaa
     3001 atattattta ttgtagctga tcaccaacac tttcagtgtt ttttttttct acttttaatg
     3061 agaattatgt tcttaggatt tatccttata aacctcttta aaaagaaatg ttatgtctgc
     3121 atagacacga catgaaatta tgaacagaaa atttaactat ttaagatcaa gacttgacat
     3181 ataacatttc taatgaacat taaacctgag gtaggcccac ttgcaaaaat gtaactgcta
     3241 tcactcactt tatacatcat tgtgtaactt gtacacacaa aaatagtgaa gcaatgtggc
     3301 atggaatgca ggtcccattc attcaaagtt attaaagcac tttcttaaag tgcattacta
     3361 ctgagctaag accagctagt tgctcataca acatactaca agtagtgtca tcctaatttg
     3421 gataaaaaaa atgtccattt tatacatgga aaagtactca ttagatcccc cattgtttgt
     3481 attacggtat ttcgtgaaca caagaggtaa atgacctaca gagctgctgt tgtgttagat
     3541 tgaaaacaca acacaggatc atggaggcat ccatcattat gacgcattct gccacttgta
     3601 actctgcaca gactttttga aaagtttaat tgagtcttgg cgttgtcgca gaaaaatacg
     3661 aaaaattctg tgttaaaatt tgcaaataaa aattccagtt ttataggaaa tcgccatggc
     3721 cttgttcttt aaccagttaa gcttcccctt ctttcactct caagttgcaa gaagcaagtg
     3781 tagcaatgtg cacgcgacag ccgggtgtgt gacgctggac caatcagagc acagagctcc
     3841 gaaagtttac cttttatggc tagagccggg tatgtgccgt catataaaag agcgcgccca
     3901 gcttttcagc ctcactttga gctcctccac acgcagctag tgcggaatat catctgcttg
     3961 taacccattc tcttaagtcg acaacccccc aaacccaagg tgagttggtt ttacagtttt
     4021 taagcgttta attatagttt ctatctatta atgttaagta aaatgtgtaa atggataaat
     4081 atgatttgat gaagtcttta gacagttcat aacggtctct ttgtagttat ctactaggtt
     4141 tgatttataa gtggtggttc ttttgataat ctatgttaca tttaataaat attggctgta
     4201 tcttaacttt tttgtagcgt tagtggatgt ggcaggtgag aatgtcggta atttaacgtg
     4261 accagtcgta ggcacgacat tgaacattga ataggccggt gtgaaatggg tgttaagtct
     4321 taatataaag ttgtgttctg gctaatctct ctttaacagc cataaatgtg atttttaact
     4381 acagttttga gggtgctggt gagcatgttg cacacttgat ggatagccgg catgggaagt
     4441 tctttgtgca ggcagtgctg cagcagggtg tgacctactt tagctagccg gctaaccagc
     4501 tctcatctga tgtaacgtaa accccatcaa tctaacatcg gctcattcgt gctttataag
     4561 atcgaagatc ttgttattag taacgggtat tggcatttag ttttaacttg gttaattttc
     4621 tttttgttat gtgttctatt taaatgtagc ctgcagcccc ctctgtcttt aatgaagctg
     4681 aagattaacg ttaacgttag tgaagtgttg tatccgctat gaaagatttg aattttagag
     4741 gtgtggtcca ctttaataac ggctgcggag ttctctggct gcacttcagc cgctgtgact
     4801 gtcgaagcag agcccatcag tttttcagga tctcggtgat ttttgaggct agactatgaa
     4861 ctgaaccgac tgagagtgac cgtccagaaa tgctttagtg tgaagtctcc gctcacgagg
     4921 agtccgtgga ctgactgcag atctgtgacg cagtaacacc cggcggcaga cacccgttga
     4981 aatgcggttg tgtaattgat cgcaggcgag ggtggaagag gatgtgaaac ttcatttgtg
     5041 taaaatttag ggagtggtcc tggtgtgatg aatgtcgaaa tctgttcctt tttactgagc
     5101 cctacgactc tggctgagtg ccacaccgcc ggcagccgca aagcggctca aaccattgcc
     5161 ttttatggta ataatgagag aatgcagagg gacttccttt gtctggcata tctgaggcgc
     5221 gcgctgtcac tagcgcccac cagcggtcag actgtagaat gcagagcaaa acaggaagtt
     5281 gactccagat ggtcacatgc ttgctgaaac gcctctccct ggcagcagtg caattctaac
     5341 agtttccctt tcttttacag ttcagcctca agtttgtaca aaaaagcagg ctcgatggag
     5401 atggcaagcc agcagggcga gacgatgtcc ggaccgctgc acattggttt gattcccaca
     5461 gttggaccgt acctgctacc gcatattatc cctatgctgc accagacctt tccaaagctg
     5521 gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga cagcggcaaa
     5581 ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga agtgccgttg
     5641 tttgatgagc caatgttgct ggctatctat gaagatcacc cgtgggcgaa ccgcgaatgc
     5701 gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg tcactgtttg
     5761 cgcgatcagg caatgtccgc cggctacaac agcgacaacg tctatatcat ggccgacaag
     5821 cagaagaacg gcatcaaggc caacttcaag atccgccaca acgtcgagga cggcagcgtg
     5881 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     5941 gacaaccact acctgagctt ccagtccgtc ctgagcaaag accccaacga gaagcgcgat
     6001 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6061 tacaacgtgg atggcggtag cggtggcacc ggcagcaagg gcgaggagct gttcaccggg
     6121 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     6181 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     6241 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg cctgaagtgc
     6301 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6361 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     6421 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcggcttc
     6481 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acggcaccgg tttctgtttt
     6541 gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggaaac tctgcgcaac
     6601 atggtggcgg caggtagcgg gatcacttta ctgccagcgc tggctgtgcc gccggagcgc
     6661 aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc
     6721 ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc
     6781 cgcgcaagaa tggatggcca tttcgataaa gttttaaaac aggcggttta acacccagct
     6841 ttcttgtaca aagtggggga tccagacatg ataagataca ttgatgagtt tggacaaacc
     6901 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6961 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     7021 tttcaggttc agggggaggt gtgggaggtt ttttccaact ttattataca tagttgataa
     7081 ttcactggcc gtcgttttac ggtaccatcg atgatgatcc agacatgata agatacattg
     7141 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     7201 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     7261 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     7321 aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc tcttgtttat
     7381 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7441 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7501 gatctacgta atacgactca ctatagttct agaggctcga gaggggccgc tttacttgta
     7561 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     7621 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
     7681 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     7741 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     7801 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     7861 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     7921 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     7981 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     8041 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     8101 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     8161 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     8221 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtcactgt
     8281 ctgctttgct gttggtctgg gctcctgggt cactggctta ctaatggagt ctttatgtat
     8341 gaggactctt atcaattgtt cttctataaa ggtctgcagt gtttctgttc gtcccctaca
     8401 tggacaccca gagcctccta aatacaggag ccctgataac tgcacaagtg ctcagattcc
     8461 agcagggtgg aaaatgagat aaagtgtgca gatggggagg gggacgtgaa tgagagattt
     8521 gagggatgaa aaggatggat gaacgcattg aaaatagccc ctttcacaaa ataataccag
     8581 taaattgtca tataattaat gaccagacct ttacctgtaa atgatgtgat gttcatatac
     8641 agaagaatgc caacaattta cagatgattt tacaaattct taattttcct tttctaaatg
     8701 gatttgttca cacattatct cttcaaagca attgaatttt ctggaaaaga ttggctgtgt
     8761 gtgtaaaagg tgctactgag gaatttaatg tgacatggaa tgaagcaaac agcagcctaa
     8821 gacaggatgg caggaaaaat gtcacatgtt taaaataaga gtgaaaccaa cgacctggac
     8881 ttatagagac tgtgtgtcct taggcaaagc tcttgtacaa acagtgcttg tgactgtgaa
     8941 attcggggtt tgcctggatt gtgttaaatg tgtgcttgga tgtatcataa aaaagaatcg
     9001 gttctctgta tttgacattt tctgtaattt atctaaagtt acaactggct atgcctgatt
     9061 tttatcacta ttgaatcaat cattttaaag aatgttaagt ctgctgattt ggagagctca
     9121 tttacattta ttttgaatgt ctcttattta acacaactga tttaagcttt agatctgcga
     9181 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
     9241 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
     9301 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
     9361 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
     9421 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
     9481 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
     9541 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
     9601 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
     9661 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
     9721 ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
     9781 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     9841 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9901 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9961 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10021 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10201 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10861 tcacgttaag gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10981 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11041 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11101 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11161 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11221 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11281 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11341 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11401 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11461 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11521 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11581 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11641 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11701 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11761 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11821 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11881 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11941 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
    12001 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
    12061 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
    12121 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
    12181 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
    12241 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaaggctg
    12301 gctaagaact catcagcctc cccggtccat ctacccacgt accaatgcac caattggcca
    12361 caatgacggc tactacatgg tgccattcct tcctctttat aggaatggag actacctcct
    12421 gtccaacaat gctcttggat acgagtacgc ctacctgttg gacccaggtc attgcacaac
    12481 accagaaatg ccctctgatc tgcaaaagac gtgaatatct gttcagacac ccatatccac
    12541 tctgttccac acaggtcaga ggtttgtcca ggagttcttg acagaggtgt aaaaagtact
    12601 caaaaatttt actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt
    12661 aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt
    12721 acttgagtat taaggaagta aaagtaaaag caagaaagaa aactagagat tcttgtttaa
    12781 gcttttaatc tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct gctttagaac
    12841 tgtttgtatt taattatcaa actataagac agacaatcta atgccagtac acgctactca
    12901 aagttgtaaa acctcagatt taacttcagt agaagctgat tctcaaaatt gttagtgtca
    12961 agcctagctc ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca
    13021 gccgatgcgg gaagaggtgt attagtcttg atagagaggc tgcaaatagc aggaaacgtg
    13081 agcagagact ccctggtgtc tgaaacacag gccagatggg ccct
//
