LOCUS       sequence-325987-       10886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Measuring cPLA2 activation in live cells after heat shock..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Enyedi B, Jelcic M, Niethammer P
  TITLE     The Cell Nucleus Serves as a Mechanotransducer of Tissue 
            Damage-Induced Inflammation.
  JOURNAL   Cell. 2016 May 19;165(5):1160-1170. doi: 10.1016/j.cell.2016.04.016.
  PUBMED    27203112
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-19- 
            19"; volume: "165"; issue: "5"; pages: "1160-1170"
COMMENT     Sequence Label: LR (hsp70I: cPLA2-mK2)
FEATURES             Location/Qualifiers
     source          1..10886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(348..1061,1062..1064,1065..1067))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(351..1061,1062..1064,1065..1067))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(393..414)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     742..761
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1001..1022
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(2290..2477,2478..2489)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2523..2539)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2523..2539)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2576..2596)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2576..2594)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2615..2631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2628..2650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2639..2655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2663..2669,2670..2687,2688..2693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2708..2729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2846..2863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3017..3605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3097..3116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3776..4567,4568..4636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4399..4418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4637..4741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4904..4925)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5378..5389,5390..5552)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5925..5941
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5961..5981
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7501..7525
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9798..10508
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKL"
     CDS             9798..10508
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKL"
     protein_bind    complement(10515..10539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10541..10675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10738..10758)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10766..10782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    join(10798..10886,1..46)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
       61 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      121 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctctt gtttattgca
      181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      301 tacgtaatac gactcactat agttctagag gctcgagagg ggccgcttta cttgtacagc
      361 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      421 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
      481 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      541 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
      601 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
      661 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
      721 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
      781 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
      841 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
      901 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
      961 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
     1021 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt cactgtctgc
     1081 tttgctgttg gtctgggctc ctgggtcact ggcttactaa tggagtcttt atgtatgagg
     1141 actcttatca attgttcttc tataaaggtc tgcagtgttt ctgttcgtcc cctacatgga
     1201 cacccagagc ctcctaaata caggagccct gataactgca caagtgctca gattccagca
     1261 gggtggaaaa tgagataaag tgtgcagatg gggaggggga cgtgaatgag agatttgagg
     1321 gatgaaaagg atggatgaac gcattgaaaa tagccccttt cacaaaataa taccagtaaa
     1381 ttgtcatata attaatgacc agacctttac ctgtaaatga tgtgatgttc atatacagaa
     1441 gaatgccaac aatttacaga tgattttaca aattcttaat tttccttttc taaatggatt
     1501 tgttcacaca ttatctcttc aaagcaattg aattttctgg aaaagattgg ctgtgtgtgt
     1561 aaaaggtgct actgaggaat ttaatgtgac atggaatgaa gcaaacagca gcctaagaca
     1621 ggatggcagg aaaaatgtca catgtttaaa ataagagtga aaccaacgac ctggacttat
     1681 agagactgtg tgtccttagg caaagctctt gtacaaacag tgcttgtgac tgtgaaattc
     1741 ggggtttgcc tggattgtgt taaatgtgtg cttggatgta tcataaaaaa gaatcggttc
     1801 tctgtatttg acattttctg taatttatct aaagttacaa ctggctatgc ctgattttta
     1861 tcactattga atcaatcatt ttaaagaatg ttaagtctgc tgatttggag agctcattta
     1921 catttatttt gaatgtctct tatttaacac aactgattta agctttagat ctgcgaagat
     1981 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     2041 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     2101 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     2161 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     2221 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     2281 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     2341 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     2401 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     2461 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     2521 cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     2581 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2641 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2701 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2761 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2821 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2881 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2941 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3661 gttaaggcat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4741 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     4801 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     4861 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     4921 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4981 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5041 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa aggctggcta
     5101 agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat
     5161 gacggctact acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc
     5221 aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca
     5281 gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat atccactctg
     5341 ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa
     5401 aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt aaaagtaaaa
     5461 gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa aattgtactt
     5521 gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt gtttaagctt
     5581 ttaatctcaa aaaacattaa atgaaatgca tacaaggttt tatcctgctt tagaactgtt
     5641 tgtatttaat tatcaaacta taagacagac aatctaatgc cagtacacgc tactcaaagt
     5701 tgtaaaacct cagatttaac ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc
     5761 tagctctttt ggggctgaaa agcaatcctg cagtgctgaa aagcctctca caggcagccg
     5821 atgcgggaag aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca
     5881 gagactccct ggtgtctgaa acacaggcca gatgggccct cgagcaggaa acagctatga
     5941 ccatgattac gccaagctat caactttgta tagaaaagtt gcttcagggg tgtcgcttgg
     6001 ttatttccaa aaatcaaatt aattttatta aactattaga acgagcatgt tttgtctata
     6061 tgctacagaa gataaaaaat aataggagtt aacagttata aaacaacaca ctttgtttct
     6121 attgattgtt gaccacactg gggtctcatt aagttagatt aaagacacac taactgggtc
     6181 aaaagcagca gattgatttc atagcaccag ggtaaacttt ctaacacttt tacggcaatc
     6241 atatacatta aaattaaata cagaccacga ctgaacaagg aggatgatct ccaatattaa
     6301 acaaagagac ttgtgcctat ttctctgagg gtaaacatga cctctcaagt tagcaagttg
     6361 gttttaacac tacaaaaata gttaagactg caatcccaga ataaagtatt ggttttaacc
     6421 aatcaatata gtacagtaaa catccatttg ttttgttgaa acgttaaaca aatctgacca
     6481 aagctattag cttatataaa acaggtttgc cttctatgta gctgaaaaca ccacaggccc
     6541 gattttgcta ctgtgtaaaa catttcagca agattttttt attgcatttt tttttactga
     6601 atcgttcaaa cattttatca ttttagtttg ttcattcatt gcaactggaa aaacaacaca
     6661 tcacacaacc gcacatattt cagcaataag tacaataaaa cactcaaata aaaaaaacat
     6721 tttaaatctc tttgtatttt tgaccgctgt ttcgcgtaat ttcacggtaa aactctggaa
     6781 atctccacta cattcctctc agcggctcct ctcaatgaca gctgaagaag tgacgcggct
     6841 gcctgctgtg ttttgattgg tcgaattcac tggaggcttc cagaacagtg tagagtctga
     6901 acgggtgcgc gctctgctgt atttaaaggg cgaaagagag accgcagaga aactcaaccg
     6961 aagagaagcg acttgacaaa gaagaaaaga gcagcctgac aggacttttt ccccgacgag
     7021 gtgtttattc gctctattta agaatctact gtaaggtaag tctcaatata ttgtactcta
     7081 ttggctaatc agaattatat agagattata tgtacttaat gtcaaaaaat caactttgta
     7141 tatgtaatct ttttacatgt ggactgccta tgttcatctt attttaggtc tactagaaaa
     7201 ttatatttcc cgttttcaca ataaggattt ttaaaaaaag caatgaacag acgggcattt
     7261 actttatgtt gctgacatta ttttatatga gcataataac cataaatact agcaaatgtc
     7321 ctaaatgaat ttgtgttaat gttgtctaca aaagaaaatt agcgttttac ttgtacaact
     7381 aataataact tggttattaa gagaatttca cttgttgact agaaaaatcc tttcataatg
     7441 aaacaattgc accataaatt gtataaatat aaaattaatt ctaattgttt ttttttcctg
     7501 ccaagtttgt acaaaaaagc aggctggacg gatctatcga tgccacaatg tccaacatta
     7561 tagttgagca tcagtattct catagactga aattgactgt ggtgagagct gaaaatgtaa
     7621 ctaaaggagc atttggggat ttattggaca ccccggaccc ttatgttgag ctgtcagtgc
     7681 caaccacacc agagtcaaga aaacgaaccc gccacattaa caatgacatt aaccccaagt
     7741 ggaacgagac ctttgagttc atattggatc ccaatcaatc caatgttttg gaggtgacac
     7801 tgatggatgc caattatgtc atggatgaaa ctcttggcac agcaaaatat tccctctcaa
     7861 aactcaaagt ggcacaaatg gagcatgtga ccctctctat cggcaagaca accaaagtgt
     7921 tcctcgactt gctcttggaa gtttgcgcat ccacagacct gcgcttcagc atgacactgt
     7981 gtgatcaaga gaagctgttc atgcagacac gcagagacag ggtcatgctc agcataaaga
     8041 agcttcttaa aatggaaaac cctcgtttcc ttccatcctc accaagagag gttcccacca
     8101 tcgctattct gggctctgga ggaggttttc gtgcaatggt gggcttctct ggtgtgatga
     8161 aggctttgta tgaatctggc gtgtttgact gtgcaacata tgtggcagga ctttcaggat
     8221 caacatggta tatgtctatg ctgtactctc atccagagtt tcctgctaaa ggcccaggcg
     8281 acattaataa agagctcatg aacagagtca gtaataaccc actcaaactg ctcctgcccc
     8341 aaaacatcaa ccgctacgtc aaagcactgt ggaagaagaa atctgctgga cagccggtca
     8401 ccttcactga catcttcggg atgttgatag gagagacgct tattccaggg agaatgaaca
     8461 ttaagctgag ctcattgaaa gggaaaatta atgaaggtca gagccctctt cctctcttca
     8521 cctgtctgca cgtcaaacct gacgtctctg agctcatgtt tgcagattgg gtggagttca
     8581 gcccttatga aattggcatg gccaaatatg gcaccttcat gtctcctggg ctgtttggca
     8641 gcaaattctt catgggaagt gttgtgaaac aatatgagga aaacccctta catttcctga
     8701 tgggtgtgtg gggaagtgct ttttccatcc tctttaaccg ggttttgggt gtgaaggaga
     8761 cgaccagcag tagcactatg gaggaggagc tggagcaaat taaaccagag cacattgtgg
     8821 gtgacgattc tgctgataac gaggaggaaa ctcaaagagg aggcacagag tctgcagatg
     8881 cagaggacga gcgccagcgt catgcccagg ccagctgggt acagcgcatg ttgacctcca
     8941 tcatgggcga caccactctg ttcaccactc gtgagggccg cgcaggcaaa gtgcacaact
     9001 tcatgctggg actgaacctg aactcaaccc tgcccttctc tcccttcagc ggcatcacac
     9061 accagacctc gctggaggag gaagtggacg ctgttactga tccggatgag tttgagcgaa
     9121 tctatgagcc tctggatgtg aagagcaaga aaatccatgt ggtggacagt ggcttgacct
     9181 ttaacctgcc ctaccctctc attctgcggt gtcagagagg agtcgacctg attatttcat
     9241 tcgacttctc agcccgaccc agtgactcca gcccaccgtt caaagagtta ctgctggctg
     9301 aaaaatgggc ccgtatgaat aagctgccct tcccgaagat tgattcgaaa gtattcgaca
     9361 gagagggtct aaaagagtgc tatgtgttca agcctgctaa aggagacaag aactgcccca
     9421 ccatcattca ttttgtcttg gccaacatca acttccggaa ttttaaagcc cctggagttc
     9481 ctcgagattc agacaaggac atagagtttg gtgactttga catatttgac gagccagcct
     9541 ccccttactc cactttcaac ttcaaataca acaaccaggc cttcaagaga cttcatgacc
     9601 tcatggagtt caatacgctt aacaacatcg aggttatcaa agaagccatt aaagacagca
     9661 ttctgctgag acgagagaac ccagcgcgat gttcggtttc cctttcgctc agtgaaatcg
     9721 aaaacaagaa gttcctgaaa cgagacaaca gcattgcaaa acgacccacc ggagctggag
     9781 gaattcgtcc tggatctatg gtgtctaagg gcgaagagct gattaaggag aacatgcaca
     9841 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
     9901 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggccgtcgag ggcggccctc
     9961 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa accttcatca
    10021 accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg
    10081 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
    10141 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc
    10201 ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagaccctg taccccgctg
    10261 acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg ggccacctga
    10321 tctgcaactt gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
    10381 gcgtctacta tgtggacaga agactggaaa gaatcaagga ggccgacaaa gagacctacg
    10441 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
    10501 aactgtaact cgagacccag ctttcttgta caaagtgggg gatccagaca tgataagata
    10561 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
    10621 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
    10681 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttccaa
    10741 ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat
    10801 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
    10861 aaatgcttta tttgtgaaat ttgtga
//
