LOCUS       sequence-326012-       13047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe 
            harbor site. This is an all-in-one doxycycline-inducible construct, 
            enabling iPSC differentiation into peripheral sensory neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Nickolls AR, Lee MM, Espinoza DF, Szczot M, Lam RM, Wang Q, Beers J,
            Zou J, Nguyen MQ, Solinski HJ, AlJanahi AA, Johnson KR, Ward ME, 
            Chesler AT, Bonnemann CG
  TITLE     Transcriptional Programming of Human Mechanosensory Neuron Subtypes 
            from Pluripotent Stem Cells.
  JOURNAL   Cell Rep. 2020 Jan 21;30(3):932-946.e7. doi: 
            10.1016/j.celrep.2019.12.062.
  PUBMED    31968264
REFERENCE   2  (bases 1 to 13047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.12"; journalName: "Cell Rep"; date: 
            "2020-01-21- 21"; volume: "30"; issue: "3"; pages: "932-946.e7"
COMMENT     Sequence Label: pUCM-CLYBL-NGN2-BRN3A
FEATURES             Location/Qualifiers
     source          1..13047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     203..222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1328..1346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1364..1382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1400..1418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1436..1454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1472..1490
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1508..1526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1544..1562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1607..1631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2557..2610
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2914..2940
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     polyA_signal    3899..4020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3936..3955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3990..4009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4069..4085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4069..4085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4082..4104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4093..4109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4117..4123,4124..4141,4142..4147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4162..4183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4300..4317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4609..4628
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    4627..4682
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     4728..4747
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4838..5584)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5616..5635)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5643..6659
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5697..5719)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6660..6938)
                     /label=chicken beta-actin promoter
     enhancer        6940..7319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7325..7549
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7538..7555
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    7571..7604
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     8258..8277
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8278..8331)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(8353..9063,9064..9066,9067..9069))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8353..9063,9064..9066,9067..9069))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8395..8416)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8744..8763
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9003..9024
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9066..9075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             9103..9371
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(9384..9595)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    9628..9719
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(9660..9679)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    9733..9781
                     /note="synthetic polyadenylation signal"
     CDS             complement(10541..10654)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             complement(10736..10789)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10813..10838
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    10839..10872
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(11730..11798,11799..12590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11948..11967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12761..13047,1..302)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
       61 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      301 aaaacgccag caacgcggcc tttttacggt tcctggcgaa ttcgccctta gagcagagcc
      361 aggaacccct gtagggaagg ggcaggagag ccaggggcat gagatggtgg acgaggaagg
      421 gggacaggga agcctgagcg cctctcctgg gcttgccaag gactcaaacc cagaagccca
      481 gagcagggcc ttagggaagc gggacggctc tgttggagag tccagtattg aattagtgat
      541 agatacacag aaaaaaagca aattgaatag gtgaatggat aaacaaactg tggaacatcc
      601 agacaatgaa atattcacca ataaaaagaa atgagctatc aagccacatt ggctatgccc
      661 ttcacattta aggaggaacc ttaaatgcat attgccaaat tttaaaagct agtctgaaaa
      721 tgctacagac tgtatgattc caattatatg acattttaga aaaggcaaaa ctatagagat
      781 cagtggttgc cagcggctca tggaggcagg atgaattggt ggaacacagg gatgctttca
      841 gggtaatgaa aatatcttgt gtgatcctgt aataatggat acgtggcata atgcatttgt
      901 taaaacctgt aaactagcca ggtatagtgg cttttttgca cctatagtct cagctacttg
      961 gtaggctgag acaggaagat cacttgagcc caggacttca agaccagcct gggtaatata
     1021 gtaagacccc atctctattt tttaaagaaa ttaagaaaga aaagaaaccc atcaactgta
     1081 taacacaaag aatcgtaatg taaactacag tcctgagtaa tgaaacattt ttaaagaaat
     1141 aaataaattc aaggcaatta ttaacttcag gaaaaacagt aaagtaaaca taagtataca
     1201 aatcaaacat ggctcagttg tgaaaatatt tacaaggtca ctttaagtaa cattgaatgt
     1261 tgatataatc ccaaaatata tttatgttgg aaggatgagg agatatctcc accccacagt
     1321 ggtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga
     1381 gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag tttactccct
     1441 atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac
     1501 agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga
     1561 gaacgtataa gctttaggcg tgtacggtgg gcgcctataa aagcagagct cgtttagtga
     1621 accgtcagat cgcctggagc aattccacaa cacttttgtc ttataccaac tttccgtacc
     1681 acttcctacc ctcgtaaact taaggttaat taagccacca tgttcgtgaa gtccgagaca
     1741 ctggaactga aagaggaaga ggacgtgctg gtgctgctgg gctctgcctc tcctgctctg
     1801 gctgctctga cacccctgag cagcagcgcc gacgaggaag aagaagagga acctggcgct
     1861 agcggcggag ccagaaggca gagaggcgct gaagctggac agggcgcaag aggcggagtg
     1921 gctgctggcg cagaaggatg cagacctgcc agactgctgg gcctggtgca cgactgcaag
     1981 agaaggccct ctagagccag agccgtgtcc agaggcgcca agacagccga gacagtgcag
     2041 cggatcaaga aaaccagacg gctgaaggcc aacaaccgcg agcggaaccg gatgcacaac
     2101 ctgaacgccg ccctggacgc cctgagagag gtgctgccta ccttccccga ggacgccaag
     2161 ctgaccaaga tcgaaaccct gagattcgcc cacaactaca tctgggccct gaccgagact
     2221 ctgcggctgg ccgatcattg tggcggcgga ggcggaggac tgccaggcgc tctgttttct
     2281 gaagccgtgc tgctgtctcc tggcggagct tctgctgccc tgtctagcag cggcgattct
     2341 cctagccctg ccagcacctg gtcctgcacc aatagcccag cccctagctc cagcgtgtcc
     2401 agcaattcca ccagccctta cagctgcacc ctgagccctg cttctccagc cggcagcgac
     2461 atggactact ggcagcctcc cccccctgac aaacatcgct acgcccccca tctgcctatc
     2521 gcccgggatt gcatcatgca cagcagaggc tctggcgagg gcagaggcag cctgctgaca
     2581 tgtggcgacg tggaagagaa ccctggcccc ggatccatga tgtccatgaa ctccaaacag
     2641 ccacactttg ccatgcaccc aactctgcct gagcataagt acccatccct gcattcctcc
     2701 tccgaggcaa tcaggagggc ttgtctgcct actcctcctc tccagagcaa cctgttcgct
     2761 tctctggacg aaacactgct cgcaagagcc gaagccctcg cagccgtgga cattgctgtt
     2821 tctcagggca agtcccatcc tttcaaaccc gacgccacct accacaccat gaatagcgtc
     2881 ccatgcacca gcacctccac tgtcccactc gctcatcatc atcaccatca ccaccaccac
     2941 caggcactgg agcctggtga tctcctcgac cacatctcct ctccatccct ggctctgatg
     3001 gctggagctg gaggtgctgg agcagctgca ggtggaggtg gagcccacga cggtccagga
     3061 ggaggtggag gaccaggagg aggaggagga cctggaggtg gtcctggtgg tggaggagga
     3121 ggaggaccag gaggaggtgg aggaggacca ggaggaggac tcctcggagg aagcgctcac
     3181 cctcatcctc acatgcacag cctgggtcac ctgtcccacc ctgctgctgc tgctgctatg
     3241 aacatgcctt ctggactgcc tcaccctggc ctggttgcag cagctgctca tcacggtgca
     3301 gctgctgcag ctgcagctgc tgctgcaggt caggtcgctg ctgcctctgc tgcagctgcc
     3361 gtcgttggag ctgctggact ggcctccatc tgcgattccg acacagaccc aagagaactc
     3421 gaagcattcg ctgaaagatt caaacaaaga agaatcaaac tcggtgttac tcaagcagat
     3481 gtgggaagcg cactggctaa cctcaagatc cctggagtcg gcagcctgag ccagtccacc
     3541 atctgtcgct ttgaatctct gaccctgtcc cacaacaaca tgattgccct caagcctatc
     3601 ctccaggctt ggctggagga ggcagaggga gcacaacgcg agaagatgaa caagccagag
     3661 ctgttcaacg gaggcgagaa gaagcgcaag cgcacttcca ttgctgcacc tgagaagaga
     3721 tccctggagg catacttcgc agtgcagccc aggccatctt ctgagaagat cgcagctatt
     3781 gccgagaagc tcgacctcaa gaagaacgtg gtcagggtgt ggttctgcaa tcagaggcag
     3841 aaacagaaga gaatgaagtt ctccgcaacc tactaatgta caatcgatcg cccaccccaa
     3901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4021 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     4081 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4141 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4201 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggacggcc gcacatgtaa
     4381 ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta
     4441 acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct gtttcatata
     4501 ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg catatgttgc
     4561 caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc catatgtcct
     4621 tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa atttccttta
     4681 ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt tggcagaggg
     4741 aaaaagatct cagtggtatt tgtgagccag ggcattggcc acaccagtca ccaccttctg
     4801 ataggcagcc tgcacctgag gagtgaattc acgcgtttta gcctggcagc atatcgagat
     4861 cgaagtcatc gagagcgtca gcagggagca tatccagatc aaaatcatcc agggcatcgg
     4921 cgggcagcat gtccaggtcg aagtcgtcca gggcgtcggt gggtccgccg ctctcgcact
     4981 tcagctgctt ctcgaggccg cagatgatca gttccaggcc gaacaggaag gcgggctcgg
     5041 cgccctgtct gtcgaacagc tcgatggcct gcttcagcag ggggggcatg ctgtcggtgg
     5101 tgggtgtctc tctttcctct ttggcgacct ggtgttcctg ttcttccagc acgcagccca
     5161 gggtgaagtg gcccacggcg ctcagggcgt acagggcgtt ttccaggctg aagccctgct
     5221 ggcacaggaa ggccagctgg ttttccagtg tctcgtactg cttctcggtg ggtctggtgc
     5281 ccaggtgcac tttggcgccg tcccggtggc tcagcagggc gcatctgtag ctcttggcgt
     5341 tgttccgcag gaagtcctgc cagctctcgc cttccagagg gcagctgtgg gtgtggtgcc
     5401 ggtccagcat ctcgatgggc agggcgtcca gcagggcccg cttgttcttc acgtgccagt
     5461 acagggtggg ctgttccacg cccagtttct gggccagctt ccgggtggtc aggccctcga
     5521 tgcccacgcc gttcagcagt tccagggcgc tgttgatcac tttgctcttg tccagccggc
     5581 tcatgtcgac attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg
     5641 agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc
     5701 acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc
     5761 ggacagcccc gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc
     5821 tccccgccca tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg
     5881 gtgcggcggc gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc
     5941 gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg
     6001 ccggcgctcc gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc
     6061 gccccgcccg gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca
     6121 cgccccgtac ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg
     6181 tgcagggggg gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac
     6241 acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc
     6301 ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag
     6361 cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg
     6421 cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt
     6481 tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga
     6541 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc
     6601 gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc
     6661 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
     6721 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
     6781 cgcccctcgc cccgccccsc cccgcctcng gcgcgcgccc cccccccccc ccgcccccat
     6841 cgctgcacaa aataattaaa aaataaataa atacaaaatt gggggtgggg agggggggga
     6901 gatggggaga gtgaagcaga acgtggggct cacctcgacc atggtaatag cgatgactaa
     6961 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     7021 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     7081 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     7141 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     7201 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     7261 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg
     7321 agacccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
     7381 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
     7441 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
     7501 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     7561 cagcgggttt ataacttcgt ataatgtatg ctatacgaag ttatggtacc ttactcgtcc
     7621 atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgaa tttaaattca
     7681 ggcgccaggc tttctggtca tgcaccaggt tctagggccc tcaggcactt ccacgtcggc
     7741 ggtcacggtg aagcccagtc tctcgtagaa gggcaggttt ctgggggcgc ttgtttccag
     7801 gaaggcgggc acgccagccc tttcagcagc ttccacgcca ggcagcacca cagcagatcc
     7861 cagtcccttg ccctggtggt caggtgacac gcccacggtg gccagaaacc aggcaggctc
     7921 tttaggtctg tggggggcca gcaggccttc catctgctgc tgggcagcca gtctagagcc
     7981 gctcagctcg gccattctag gtccgatctc ggcgaacaca gcgccggctt ccacagactc
     8041 aggggttgtc cacacagcca cagcggcgcc atcatcggcc acccacactt tgccgatgtc
     8101 caggcccact ctggtcagaa acagttcctg cagctcggtc actctctcga tgtgtctgtc
     8161 ggggtccacg gtgtgtctgg tggcagggta atcggcgaag gcagcggcca gtgtccgcac
     8221 agctctaggc acatcgtccc tggtggccag ccgcactgtg ggcttgtact cggtcatagg
     8281 gccgggattc tcctccacgt caccgcatgt tagaagactt cctctgccct cgccagagcc
     8341 tctgctgtgc atcttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     8401 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
     8461 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     8521 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     8581 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     8641 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     8701 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     8761 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     8821 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     8881 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     8941 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     9001 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     9061 gctcaccatg gtggcgctag cgtaggcgcc ggtcacagct tggatctgta acggcgcaga
     9121 acagaaaacg aaacaaagac gtagagttga gcaagcaggg tcaggcaaag cgtggagagc
     9181 cggctgagtc taggtaggct ccaagggagc gccggacaaa ggcccggtct cgacctgagc
     9241 tttaaactta cctagacggc ggacgcagtt caggaggcac cacaggcggg aggcggcaga
     9301 acgcgactca accggcgtgg atggcggcct caggtagggc ggcgggcgcg tgaaggagag
     9361 atgcgagccc ctcgaagctt cagctgtgtt ctggcggcaa acccgttgcg aaaaagaacg
     9421 ttcacggcga ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc
     9481 cagtacacga catcactttc ccagtttacc ccgcgccacc ttctctaggc accggttcaa
     9541 ttgccgaccc ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga
     9601 cgggcaccgg agcgatcgca gatcgagaga gaaatgttct ggcacctgca cttgcactgg
     9661 ggacagccta ttttgctagt ttgttttgtt tcgttttgtt ttgatggaga gcgtatgtta
     9721 gtactatcga ttcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat
     9781 ttcaccgctt gaaggcgatg tatgccacca gtccgacgac gacggctgcc aggatggagc
     9841 aatacacggg aatcaggtta tctgtggtgc cccgggtgac cactggctgg gagctcccca
     9901 tgacagttgt gaccacgcca gccacggtgg aggcgatcag atcctgctct ggtggtgctt
     9961 ctggctcctg tgtagatggt gcggtgctgt ctgatccttc agggggggtg gaccgagtaa
    10021 tccatcgccc tgggatttcc tcgcactcgg cgtctgccca tctggtacat tcgcgcagct
    10081 gcctttcggt atcctcgcac acagtacaag gcaggcaggg gtccacgtga ttggcctcat
    10141 cgctatatgt cccgtcagga cattcctcgc acacggtgtt ctgtttatcc tggcagctaa
    10201 agaccagccc tgagcctgcc tcacacaccc tgcaagcttc acatctgccg gtagtctcgt
    10261 cctgatagta tccgtatgca caccggcaga ctgcatcgtc agcctccaca caaggagcac
    10321 tcatagactg caggcccacg cactcagtac atggcttgca aggttctgtg gcactgacca
    10381 catcagagaa tgtgacggag tccagacaag gctcgcacac agtctgattg gctccgcatg
    10441 gctgtgccac tccctctccc aggttacaag ctttacagca ttctccagaa tgagtgtaca
    10501 gccctgtggg gcaggcttcc ttcgcgatcg ctccacttcc tggctctcgc tgtccctgag
    10561 gatggtgttc cagtctagcc cgcagctgtt ccagctctcc tgccagtccc tcgaccacgt
    10621 gtcctcctcg ccatcctgtg gtcttttcgt ccatccctga cccctcgagc tgcacctgtg
    10681 aatgcactcc agttgcggtt gcgaccagaa acagaatgat acatgaccag cccatagggc
    10741 cgggattctc ctccacgtca ccgcatgtta gaagacttcc tctgccctct ccgctgccag
    10801 atctctcgag gccctgtggg aggaagagaa gaggtcagat aacttcgtat aatgtatgct
    10861 atacgaagtt atgcggccgc tgggagaagg gtaagagagc tcaatcctca tcttacatag
    10921 aaggaagtca gtagataata tcttacattt aaaagtcaag aaataacagt ctgaccatac
    10981 tatctagaaa tatggaagtt aacagtaagg aagttaaaac agttgaaagt agtttcgtct
    11041 agaaagaaag acttggggaa gaacaaagaa ggctaatttt cattatgagc tttattgtaa
    11101 cagaaaagag accactaact cagctaggtt ttcccaattc ctcaacactt tggggcacag
    11161 tgtttagtca gagctgtgat cactctctga gcacatggat tgtatcttaa ttttcatctc
    11221 attgaaaata tgtaccattt gtccaagtgg aaactgggct gaagcatgac tgtgaccacc
    11281 accaaagaaa tggctagact cgctaaggtc tgctggggtg acccgtgatt gtgttgtgct
    11341 tgatcttgtt gagcatggct cttttaagtc accatacctg cccctgccaa tgcaccaaac
    11401 accaacttaa agggactttc agcttttgta agtcagtctc agccttactt ctgaaccatt
    11461 tctttgactt ctttcatgac tctgatcctg tcccagtttt aagcctccca gccaccagtc
    11521 atgctgtttt gctctgctat ttacattctt ctctacctac tttggacttg atggtatgcc
    11581 tactatatta gttatttatt gctgtgtaac aaaccactct gaaacatagt ggcttacaac
    11641 cacaaccatt ttattttatt atgaaactgt gggtcagaat gtatattggc ccattggcat
    11701 gattctcctg ctattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    11761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    11821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    11881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    11941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    12001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    12061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    12121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    12181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    12241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    12301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    12361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    12421 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    12481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    12541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    12601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    12661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    12721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    12781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    12841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    12901 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    12961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    13021 gtcttaccgg gttggactca agacgat
//
