LOCUS       sequence-326014-        3504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Batis maritima MHT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Fulk EM, Huh D, Atkinson JT, Lie M, Masiello CA, Silberg JJ
  TITLE     A Split Methyl Halide Transferase AND Gate That Reports by 
            Synthesizing an Indicator Gas.
  JOURNAL   ACS Synth Biol. 2020 Nov 20;9(11):3104-3113. doi: 
            10.1021/acssynbio.0c00355. Epub 2020 Oct 26.
  PUBMED    33104325
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00355"; journalName: "ACS Synth Biol"; date: 
            "2020-11-20- 20"; volume: "9"; issue: "11"; pages: "3104-3113"
COMMENT     Sequence Label: pEMF037
FEATURES             Location/Qualifiers
     source          1..3504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(68..162)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(183..842)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     757..776
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1011..1029
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1050..1068,1069..1074,1075..1091,1092..1097,
                     1098..1123)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1050..1068
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1075..1093
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1944..1978
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             2013..2639
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2089..2108)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      2645..2731
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2896..3484)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2976..2995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgactag
       61 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      121 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      181 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      241 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      301 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      361 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
      421 ttctcaataa accctttagg gaaataggcc aggttttcac cataacacgc cacatcttgc
      481 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
      541 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
      601 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      661 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      721 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      781 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      841 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgac cggtagtgat
      901 cttatttcat tatggtgaaa gttggaacct cttacgggcc ggccaacgtc tcattttcgc
      961 cagatatcga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1021 tcacgaggcc ctttcgtctt cacctcgagt ccctatcagt gatagagatt gacatcccta
     1081 tcagtgatag agatactgag cacatcagca ggacgcactg accgatagga cccatacaaa
     1141 ggaagttttg atgagcaccg tggcgaacat tgcgccggtg tttaccggcg attgcaaaac
     1201 cattccgacc ccggaagaat gcgcgacctt tctgtataaa gtggtgaaca gcggcggctg
     1261 ggaaaaatgc tgggtggaag aagtgattcc gtgggatctg ggcgtgccga ccccgctggt
     1321 gctgcatctg gtgaaaaaca acgcgctgcc gaacggcaaa ggcctggtgc cgggctgcgg
     1381 cggcggctat gatgtggtgg cgatggcgaa cccggaacgc tttatggtgg gcctggatat
     1441 tagcgaaaac gcgctgaaaa aagcgcgcga aacctttagc accatgccga acagcagctg
     1501 ctttagcttt gtgaaagaag atgtgtttac ctggcgcccg gaacagccgt ttgattttat
     1561 ttttgattat gtgttttttt gcgcgattga tccgaaaatg cgcccggcgt ggggcaaagc
     1621 gatgtatgaa ctgctgaaac cggatggcga actgattacc ctgatgtatc cgattaccaa
     1681 ccatgaaggc ggcccgccgt ttagcgtgag cgaaagcgaa tatgaaaaag tgctggtgcc
     1741 gctgggcttt aaacagctga gcctggaaga ttatagcgat ctggcggtgg aaccgcgcaa
     1801 aggcaaagaa aaactggcgc gctggaaaaa aatgaacaac taataatcta actaaaaagg
     1861 cctcccaaat cggggggcct tttttattga taacaaaagg tacccgtgga tcctctagag
     1921 tcgacctgca ggcatgcaag ctttttacgg ctagctcagt cctaggtata gtgctagccc
     1981 agccagagaa acactcttta acagggggta gtatgatgtc tcgtttagat aaaagtaaag
     2041 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
     2101 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
     2161 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
     2221 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
     2281 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
     2341 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
     2401 atgcattata tgcactcagc gcagtggggc attttacttt aggttgcgta ttggaagatc
     2461 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
     2521 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
     2581 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcttaag
     2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2701 gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagacctagg gcgttcggct
     2761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2821 taacgcatgc aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3301 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3421 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3481 tcaagaagat cctttgatct tttc
//
