LOCUS       sequence-326034-        4920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replaces Addgene plasmid 118459. Yeast tagging vector to create a 
            gene fusion with yeast optimized mVenus with CaUra3 selection; 
            Contains the linker region at the 5' end of the FP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-link-ymVenus-URA3
FEATURES             Location/Qualifiers
     source          1..4920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1106..1822
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1863..2050
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1918..1942)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2125..2468
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3287..3484
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3591..3610)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3592..3610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3697..3714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3868..4456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3948..3967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4627..4920,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaacatgtc taaaggtgaa gaattattca ctggtgttgt
     1141 cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga
     1201 aggtgaaggt gatgctactt acggtaaatt gaccttaaaa ttgatttgta ctactggtaa
     1261 attgccagtt ccatggccaa ccttagtcac tactttaggt tatggtttgc aatgttttgc
     1321 tagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta
     1381 tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt
     1441 caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga
     1501 agatggtaac attttaggtc acaaattgga atacaactat aactctcaca atgtttacat
     1561 cactgctgac aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga
     1621 agatggtggt gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc
     1681 agtcttgtta ccagacaacc attacttatc ctatcaatct aaattatcca aagatccaaa
     1741 cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta ttacccatgg
     1801 tatggatgaa ttgtacaaat aagcggccgc ctcgaggtcg acggcgcgcc acttctaaat
     1861 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     1921 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     1981 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
     2041 cctctaccgg cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg
     2101 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     2161 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     2221 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     2281 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     2341 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     2401 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     2461 aaacaaccat gacagtcaac actaagacct atagtgagag agcagaaact catgcctcac
     2521 cagtagcaca gcgattattt cgattaatgg aactgaagaa aaccaattta tgtgcatcaa
     2581 ttgacgttga taccactaag gaattccttg aattaattga taaattaggt ccttatgtat
     2641 gcttaatcaa gactcatatt gatataatca atgatttttc ctatgaatcc actattgaac
     2701 cattattaga actttcacgt aaacatcaat ttatgatttt tgaagataga aaatttgctg
     2761 atattggtaa taccgtaaag aaacaatata ttggtggagt ttataaaatt agtagttggg
     2821 cagatattac caatgctcat ggtgtcactg ggaatggagt ggttgaagga ttaaaacagg
     2881 gagctaaaga aaccaccacc aaccaagagc caagagggtt attgatgtta gctgaattat
     2941 catcagtggg atcattagca tatggagaat attctcaaaa aactgttgaa attgctaaat
     3001 ccgataagga atttgttatt ggatttattg cccaacgtga tatgggtggc caagaagaag
     3061 gatttgattg gcttattatg acacctggag ttggattaga tgataaaggt gatggattag
     3121 gacaacaata tagaactgtt gatgaagttg ttagcactgg aactgatatt atcattgttg
     3181 gtagaggatt gtttggtaaa ggaagagatc cagatattga aggtaaaagg tatagaaatg
     3241 ctggttggaa tgcttatttg aaaaagactg gccaattata atcagtactg acaataaaaa
     3301 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     3361 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     3421 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     3481 actgctgtcg attcgatact aacgccgcca tccagtgtcg aaaacgagct cgaattcatc
     3541 gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg
     3601 agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg
     3661 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3721 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3781 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3901 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3961 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4021 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     4081 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4141 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4201 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4261 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4321 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4381 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4441 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4501 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4561 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4621 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4681 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4741 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4801 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4861 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
//
