LOCUS       sequence-326038-        7717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sMHT-113 fused to CheA and CheY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Fulk EM, Huh D, Atkinson JT, Lie M, Masiello CA, Silberg JJ
  TITLE     A Split Methyl Halide Transferase AND Gate That Reports by 
            Synthesizing an Indicator Gas.
  JOURNAL   ACS Synth Biol. 2020 Nov 20;9(11):3104-3113. doi: 
            10.1021/acssynbio.0c00355. Epub 2020 Oct 26.
  PUBMED    33104325
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00355"; journalName: "ACS Synth Biol"; date: 
            "2020-11-20- 20"; volume: "9"; issue: "11"; pages: "3104-3113"
COMMENT     Sequence Label: pDH007
FEATURES             Location/Qualifiers
     source          1..7717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(54..72)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      68..115
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      152..607
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(239..258)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     449..470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(699..1514)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(699..1514)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1506..1525
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1636..2224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2125..2144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2378..2395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2410..2549
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2475..2494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2635..2657
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2651..2842)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3651..4733)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4695..4714
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4734..4811)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5017..5036
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5120..5138
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5139..5163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5178..5200
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5219..5236
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5246..5263
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5267..5299
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        5969..6003
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             6021..6833
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKT
                     AFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDAS
                     DFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIA
                     DRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6021..6833
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKT
                     AFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDAS
                     DFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIA
                     DRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        6841..6859
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6860..6884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6899..6921
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(7701..7717,1)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
       61 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      121 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      181 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      241 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      301 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      361 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      421 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      481 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
      541 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
      601 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      661 aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc
      721 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      781 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      841 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      901 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      961 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     1021 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     1081 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     1141 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     1201 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     1261 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     1321 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1381 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1441 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
     1501 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2401 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     2461 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     2521 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     2581 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2641 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     2701 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     2761 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     2821 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     2881 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     2941 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     3001 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     3061 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     3121 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     3181 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     3241 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     3301 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     3361 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     3421 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     3481 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     3541 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     3601 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     3661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3721 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     3781 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     3841 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     3901 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     3961 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     4021 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     4081 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     4141 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     4201 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     4261 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     4321 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     4381 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     4441 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     4501 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     4561 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     4621 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     4681 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     4741 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     4801 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     4861 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     4921 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     4981 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     5041 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     5101 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     5161 tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
     5221 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg ctagcatgac
     5281 tggtggacag caaatgggtg cgggatccat gagcaccgtg gcgaacattg cgccggtgtt
     5341 taccggcgat tgcaaaacca ttccgacccc ggaagaatgc gcgacctttc tgtataaagt
     5401 ggtgaacagc ggcggctggg aaaaatgctg ggtggaagaa gtgattccgt gggatctggg
     5461 cgtgccgacc ccgctggtgc tgcatctggt gaaaaacaac gcgctgccga acggcaaagg
     5521 cctggtgccg ggctgcggcg gcggctatga tgtggtggcg atggcgaacc cggaacgctt
     5581 tatggtgggc ctggatatta gcgaaaacgc gctgaaaaaa gcgcgcgaaa cctttagcac
     5641 catgccggcg gccgcgggtg cttctggtgg tggttcttct ggtggtcaca tgggtttcaa
     5701 aacgttttat attaaagtga ttctcaaaga aggaacgcag ttgaaatccg ccaggattta
     5761 cctcgttttc cacaagcttg aagaactgaa gtgtgaagtt gtgagaacga ttccttcggt
     5821 tgaagagata gaagaagaga aattcgaaaa cgaagtggaa ctcttcgtaa tctcccctgt
     5881 ggatctggag aaactctctg aagctctgtc aagcatcgcc gatatagaga gggtaataat
     5941 aaaagaagta accgccgtca cctaatgatt tacggctagc tcagtcatag gtatagtgct
     6001 agcaggaggt gtactagttg agccatattc aacgggaaac gtcttgctct aggccgcgat
     6061 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     6121 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
     6181 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     6241 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     6301 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg
     6361 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     6421 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     6481 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     6541 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     6601 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     6661 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     6721 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     6781 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taactgcagt
     6841 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcaa gaaataattt
     6901 tgtttaactt taagaaggag atatacatat gggaaagaga gttttgatag tcgatgatgc
     6961 agcgttcatg aggatgatgt tgaaagatat catcacaaaa gcgggatacg aagttgcagg
     7021 ggaagcaaca aacggtcgtg aagccgttga aaaatacaag gaactcaaac cggacatcgt
     7081 cacgatggac atcaccatgc cggagatgaa cggtatcgat gcgatcaaag agatcatgaa
     7141 gatcgacccc aacgcaaaga tcatcgtctg cagtgccatg ggacagcagg caatggtcat
     7201 agaagctata aaagctggag cgaaagactt cattgtgaaa cccttccagc cttccagggt
     7261 ggtagaggct ctcaacaagg tttcgaaggg tgcttctggt ggtggttctt ctggtggtca
     7321 catggcggcc gcaaacagca gctgctttag ctttgtgaaa gaagatgtgt ttacctggcg
     7381 cccggaacag ccgtttgatt ttatttttga ttatgtgttt ttttgcgcga ttgatccgaa
     7441 aatgcgcccg gcgtggggca aagcgatgta tgaactgctg aaaccggatg gcgaactgat
     7501 taccctgatg tatccgatta ccaaccatga aggcggcccg ccgtttagcg tgagcgaaag
     7561 cgaatatgaa aaagtgctgg tgccgctggg ctttaaacag ctgagcctgg aagattatag
     7621 cgatctggcg gtggaaccgc gcaaaggcaa agaaaaactg gcgcgctgga aaaaaatgaa
     7681 caactaaaag cttactcgag caccaccacc accacca
//
