LOCUS       sequence-326078-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For doxycycline inducible mammalian expression of EGFP tagged 
            Spastin M87 with exon 4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Cupido T, Pisa R, Kelley ME, Kapoor TM
  TITLE     Designing a chemical inhibitor for the AAA protein spastin using 
            active site mutations.
  JOURNAL   Nat Chem Biol. 2019 May;15(5):444-452. doi: 
            10.1038/s41589-019-0225-6. Epub 2019 Feb 18.
  PUBMED    30778202
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0225-6"; journalName: "Nat Chem Biol"; date: 
            "2019-05"; volume: "15"; issue: "5"; pages: "444-452"
COMMENT     Sequence Label: HA, EGFP Spastin M87 with exon 4
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..35
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 anangcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
       61 tggacgagct gtacaaggtc gacgccggca tggcagccaa gaggagctcc ggggccgcgc
      121 cagcacctgc ctcggcctcg gccccggcgc cggtgccggg cggcgaggcc gagcgcgtcc
      181 gagtcttcca caaacaggcc ttcgagtaca tctccattgc cctgcgcatc gatgaggatg
      241 agaaagcagg acagaaggag caagctgtgg aatggtataa gaaaggtatt gaagaactgg
      301 aaaaaggaat agctgttata gttacaggac aaggtgaaca gtgtgaaaga gctagacgcc
      361 ttcaagctaa aatgatgact aatttggtta tggccaagga ccgcttacaa cttctagaga
      421 agatgcaacc agttttgcca ttttccaagt cacaaacgga cgtctataat gacagtacta
      481 acttggcatg ccgcaatgga catctccagt cagaaagtgg agctgttcca aaaagaaaag
      541 accccttaac acacactagt aattcactgc ctcgttcaaa aacagtcatg aaaactggat
      601 ctgcaggcct ttcaggccac catagagcac ctagttacag tggtttatcc atggtttctg
      661 gagtgaaaca gggatctggt cctgctccta ccactcataa gggtactccg aaaacaaata
      721 ggacaaataa accttctacc cctacaactg ctactcgtaa gaaaaaagac ttgaagaatt
      781 ttaggaatgt ggacagcaac cttgctaacc ttataatgaa tgaaattgtg gacaatggna
      841 ncagctgtta aatttgatga tatagctggt caagacttgg caaaacaagc attgcaagaa
      901 attgttattc ttcctnnnct gaggcctgag ttgttcacag ggcttanagc tcctgccaga
      961 gggctgttac tctttggtcc anctgggaat gnnaganatg ctggctaaag cagtagctgc
     1021 nnaancgaat gcnncnttct ttantnnnan ng
//
