LOCUS       sequence-326081-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For doxycycline inducible mammalian expression of EGFP tagged 
            Spastin M87 with exon 4, with N386C mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Cupido T, Pisa R, Kelley ME, Kapoor TM
  TITLE     Designing a chemical inhibitor for the AAA protein spastin using 
            active site mutations.
  JOURNAL   Nat Chem Biol. 2019 May;15(5):444-452. doi: 
            10.1038/s41589-019-0225-6. Epub 2019 Feb 18.
  PUBMED    30778202
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0225-6"; journalName: "Nat Chem Biol"; date: 
            "2019-05"; volume: "15"; issue: "5"; pages: "444-452"
COMMENT     Sequence Label: HA, EGFP Spastin M87 with exon 4, N386C
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..30
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
       61 gagctgtaca aggtcgacgc cggcatggca gccaagagga gctccggggc cgcgccagca
      121 cctgcctcgg cctcggcccc ggcgccggtg ccgggcggcg aggccgagcg cgtccgagtc
      181 ttccacaaac aggccttcga gtacatctcc attgccctgc gcatcgatga ggatgagaaa
      241 gcaggacaga aggagcaagc tgtggaatgg tataagaaag gtattgaaga actggaaaaa
      301 ggaatagctg ttatagttac aggacaaggt gaacagtgtg aaagagctag acgccttcaa
      361 gctaaaatga tgactaattt ggttatggcc aaggaccgct tacaacttct agagaagatg
      421 caaccagttt tgccattttc caagtcacaa acggacgtct ataatgacag tactaacttg
      481 gcatgccgca atggacatct ccagtcagaa agtggagctg ttccaaaaag aaaagacccc
      541 ttaacacaca ctagtaattc actgcctcgt tcaaaaacag tcatgaaaac tggatctgca
      601 ggcctttcag gccaccatag agcacctagt tacagtggtt tatccatggt ttctggagtg
      661 aaacagggat ctggtcctgc tcctaccact cataagggta ctccgaaaac aaataggaca
      721 aataaacctt ctacccctac aactgctact cgtaagaaaa aagacttgaa gaattttang
      781 aatgtggaca gcaaccttgc taaccttata atgaatgaaa ttgtggacaa tggaacagct
      841 gttaaatttg atgatatagc tggtcaagac ttggcaaaac aagcattgca agaaattgtt
      901 attcttcctt ctctgnnnct gagttgttca cagggcttan agctcctgcc agagggctgt
      961 tactctttgg tccacctggg tgngggaann anaatgctgg c
//
