LOCUS       sequence-326082-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For doxycycline inducible mammalian expression of EGFP tagged 
            Spastin M87 with exon 4, with N386C mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Cupido T, Pisa R, Kelley ME, Kapoor TM
  TITLE     Designing a chemical inhibitor for the AAA protein spastin using 
            active site mutations.
  JOURNAL   Nat Chem Biol. 2019 May;15(5):444-452. doi: 
            10.1038/s41589-019-0225-6. Epub 2019 Feb 18.
  PUBMED    30778202
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0225-6"; journalName: "Nat Chem Biol"; date: 
            "2019-05"; volume: "15"; issue: "5"; pages: "444-452"
COMMENT     Sequence Label: HA, EGFP Spastin M87 with exon 4, N386C
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cnctagactc gagcggccgc ttaacagtgg tatctccaaa gtccttgttc caacgtatgt
       61 acgcttctaa agtttgaggg ctgacgctgc gttttatttt tttcaaggat tcagtgaagt
      121 cagataatcg aatatttctc atctcactgg cagacatatt cttcacctgt tctggtttta
      181 gttctcggat aggacccagt gctgcatctt ttgccaaagc tgttaggtca cttcctgagt
      241 atccatcagt cattctagca agttgtgcta gttctttttg ggtcaatgga cttccttgtt
      301 tacataacag atttttaagc aaaagtagtc ttgtctcctc atttggtaaa gacacatata
      361 cccgtttgat aaaacgcctg agaacagcct catcaagctc ttgtggccta ttagttgcac
      421 ccattacaag tactctgtca tctccagcag actgtacacc atcaaattct attagaaatt
      481 cagtttttag gcgtctacta gcatcgtgct ccccttctct tctttcacac aaaaggctat
      541 caacttcatc tataaaaatt atagaaggtt gaagttctcg agccacagca aaaagagccc
      601 tcaccaattt ctctccttct cccacgtatt ttgaagttaa acttgcagca cttatattaa
      661 agaaggttgc attcgattct gcagctactg ctttagccag cattgtcttc ccacacccag
      721 gtggaccaaa gagtaacagc cctctggcag gagctctaag ccctgtgaac aactcnngcc
      781 tcagagaang aagaataaca atttcttgca atgcttgttt tgccaagtct tgaccagcta
      841 tatcatcaaa tttaacagct gttccattgt ccacaatttc attcattata nnntagcaag
      901 gttgctgtcc acattcctaa aattcttcaa gtcttttttc ttacgagtag cagttgtngg
      961 ggt
//
