LOCUS       sequence-326083-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For doxycycline inducible mammalian expression of EGFP tagged 
            Spastin M87 with exon 4, with N386C mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Cupido T, Pisa R, Kelley ME, Kapoor TM
  TITLE     Designing a chemical inhibitor for the AAA protein spastin using 
            active site mutations.
  JOURNAL   Nat Chem Biol. 2019 May;15(5):444-452. doi: 
            10.1038/s41589-019-0225-6. Epub 2019 Feb 18.
  PUBMED    30778202
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0225-6"; journalName: "Nat Chem Biol"; date: 
            "2019-05"; volume: "15"; issue: "5"; pages: "444-452"
COMMENT     Sequence Label: HA, EGFP Spastin M87 with exon 4, N386C
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagnnnnnnn gangactaat ttggttatgg ccaaggaccg cttacaactt ctagagaaga
       61 tgcaaccagt tttgccattt tccaagtcac aaacggacgt ctataatgac agtactaact
      121 tggcatgccg caatggacat ctccagtcag aaagtggagc tgttccaaaa agaaaagacc
      181 ccttaacaca cactagtaat tcactgcctc gttcaaaaac agtcatgaaa actggatctg
      241 caggcctttc aggccaccat agagcaccta gttacagtgg tttatccatg gtttctggag
      301 tgaaacaggg atctggtcct gctcctacca ctcataaggg tactccgaaa acaaatagga
      361 caaataaacc ttctacccct acaactgcta ctcgtaagaa aaaagacttg aagaatttta
      421 ggaatgtgga cagcaacctt gctaacctta taatgaatga aattgtggac aatggaacag
      481 ctgttaaatt tgatgatata gctggtcaag acttggcaaa acaagcattg caagaaattg
      541 ttattcttcc ttctctgagg cctgagttgt tcacagggct tagagctcct gccagagggc
      601 tgttactctt tggtccacct gggtgtggga agacaatgct ggctaaagca gtagctgcag
      661 aatcgaatgc aaccttcttt aatataagtg ctgcaagttt aacttcaaaa tacgtgggag
      721 aaggagagaa attggtgagg gctctttttg ctgtggctcg agaacttcaa ccttctataa
      781 tttttataga tgaagttgat agccttttgt gtgaaagaag agaaagggag cacgatgcta
      841 gtagacgcct aaaaactgaa tttctaatag aatttgatgg tgtacagtct gctggagatg
      901 acagagtact tgtaatgggt gcaactaata ggncacaaga gcttgatgag gctgttctca
      961 ggcgttttat caaacgggta ta
//
