LOCUS       sequence-326140-        7608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Mauger DM, Lin C, Garcia-Blanco MA
  TITLE     hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth 
            factor receptor 2 exon IIIc.
  JOURNAL   Mol Cell Biol. 2008 Sep . 28(17):5403-19.
  PUBMED    18573884
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Sep . 28(17):5403-19."
COMMENT     Sequence Label: p3x-FLAG-hnRNPH1
FEATURES             Location/Qualifiers
     source          1..7608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2366..2988
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2565..2584)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3012..3032)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3030..3327
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3381..4175
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3435..3454)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4045..4064
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4827..4961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4864..4883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4918..4937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4966..4985)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4967..4985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4999..5015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4999..5015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5012..5034)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5023..5039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5047..5053,5054..5071,5072..5077))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5092..5113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5402..5990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5482..5501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(7022..7126)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7153..7581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7240..7259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7450..7471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgat gatgttgggc
     1021 acggaaggtg gagagggatt cgtggtgaag gtccggggct tgccctggtc ttgctcggcc
     1081 gatgaagtgc agaggttttt ttctgactgc aaaattcaaa atggggctca aggtattcgt
     1141 ttcatctaca ccagagaagg cagaccaagt ggcgaggctt ttgttgaact tgaatcagaa
     1201 gatgaagtca aattggccct gaaaaaagac agagaaacta tgggacacag atatgttgaa
     1261 gtattcaagt caaacaacgt tgaaatggat tgggtgttga agcatactgg tccaaatagt
     1321 cctgacacgg ccaatgatgg ctttgtacgg cttagaggac ttccctttgg atgtagcaag
     1381 gaagaaattg ttcagttctt ctcagggttg gaaatcgtgc caaatgggat aacattgccg
     1441 gtggacttcc aggggaggag tacgggggag gccttcgtgc agtttgcttc acaggaaata
     1501 gctgaaaagg ctctaaagaa acacaaggaa agaatagggc acaggtatat tgaaatcttt
     1561 aagagcagta gagctgaagt tagaactcat tatgatccac cacgaaagct tatggccatg
     1621 cagcggccag gtccttatga cagacctggg gctggtagag ggtataacag cattggcaga
     1681 ggagctggct ttgagaggat gaggcgtggt gcttatggtg gaggctatgg aggctatgat
     1741 gattacaatg gctataatga tggctatgga tttgggtcag atagatttgg aagagacctc
     1801 aattactgtt tttcaggaat gtctgatcac agatacgggg atggtggctc tactttccag
     1861 agcacaacag gacactgtgt acacatgcgg ggattacctt acagagctac tgagaatgac
     1921 atttataatt ttttttcacc gctcaaccct gtgagagtac acattgaaat tggtcctgat
     1981 ggcagagtaa ctggtgaagc agatgtcgag ttcgcaactc atgaagatgc tgtggcagct
     2041 atgtcaaaag acaaagcaaa tatgcaacac agatatgtag aactcttctt gaattctaca
     2101 gcaggagcaa gcggtggtgc ttacgaacac agatatgtag aactcttctt gaattctaca
     2161 gcaggagcaa gcggtggtgc ttatggtagc caaatgatgg gaggcatggg cttgtcaaac
     2221 cagtccagct acgggggccc agccagccag cagctgagtg ggggttacgg aggcggctac
     2281 ggtggccaga gcagcatgag tggatacgac caagttttac aggaaaactc cagtgatttt
     2341 caatcaaaca ttgcataggg atcccgggtg gcatccctgt gacccctccc cagtgcctct
     2401 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     2461 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     2521 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     2581 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     2641 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     2701 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     2761 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     2821 cactgctccc ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca
     2881 gacacagcat aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg
     2941 cactgtcctc tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc
     3001 agcttggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3301 ccagaagtag tgaggaggct tttttggagg aattgatcag cttgggatct gatcaagaga
     3361 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3421 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3481 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3541 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     3601 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3661 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3721 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3781 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3841 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3901 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3961 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4021 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4081 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4141 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     4201 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4261 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4321 tctcatgctg gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt ggttcagctg
     4381 ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca
     4441 ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac
     4501 gatggccgct ttggtcgacc cggacgggac gctcctgcgc ctgatacaga acgaattgct
     4561 tgcaggcatc tcatgagtgt gtcttcccgt tttccgcctg aggtcactgc gtggatggag
     4621 cgctggcgcc tgctgcgcga cggcgagctg ctcaccaccc actcgccaag ctggaaccgt
     4681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc gatcataatc agccatacca
     4741 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4801 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     4861 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4921 gtttgtccaa actcatcaat gtatcttatc atgtctggat caattcccta tagtgagtcg
     4981 tattaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5041 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5101 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgts
     5161 ycatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5221 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgagcggtat
     5281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5821 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6721 ccaagtcatt ctgagaatag tgtatgcggc gagcggtatc agctcactca aaggcggtaa
     6781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt yagttcgatg taacccactc
     6901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7141 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7201 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7261 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7321 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7381 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7441 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7501 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7561 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaattt
//
