LOCUS       sequence-32621-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Jin SW, Beis D, Mitchell T, Chen JN, Stainier DY
  TITLE     Cellular and molecular analyses of vascular tube and lumen formation
            in zebrafish.
  JOURNAL   Development. 2005 Dec . 132(23):5199-209.
  PUBMED    16251212
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2005 Dec . 132(23):5199-209."
COMMENT     Sequence Label: flk1 promoter
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     91..107
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     92..108
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgccctttaa tacgactcac
       61 tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat aagctttttc
      121 ttttatttaa aattaaatgt aatatattca caaataaaaa aaaacaacta atttacaact
      181 atttaaacta aatgagccat tttggtgaaa agattttgat ataaattata aaaaaaaagt
      241 tttaacaaat ttaattcacg atcatccaag ttaactgaat aattgctgtt tgtgaatcgg
      301 cagccgttca ttttcactga gcaatgttat tttatttaaa cagaccctat acacagattt
      361 tacaacatat gatttaaaaa atgtataaac gagatatatg attaatatag gcttcttggc
      421 attggtcagg gacccataat tccctggggc cctaggcggc tgctaacttt gattattgat
      481 taagtccgct cctgctagta tgttcatttg tgcgtccata aaaggctaat ttaaacatgt
      541 ttaaaattgt attaactaca ttgaccaatt attttaattt attaaacatt tctacatttc
      601 ccagaccgca gattgaaacc cccaacagta ttaaatagat acttaataca ggcaagtaaa
      661 atcattatta attataatta ttaggcctaa ttaatacatg tattattatt taaaaaaaat
      721 ctaatttata ttaaagttaa taaaacaaaa gactaataca aggacagacc tttggccttc
      781 tgctcttctg taattgaagt gccaacacgt ttggtgacag cccagttcca caactggatg
      841 gcgatttcct ccagctacaa aacaactaaa ttattaaata ttgaa
//
