LOCUS       sequence-326212-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wild‐type E. coli lipocalin Blc without signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Bozhanova NG, Calcutt MW, Beavers WN, Brown BP, Skaar EP, Meiler J
  TITLE     Lipocalin Blc is a potential heme-binding protein.
  JOURNAL   FEBS Lett. 2021 Jan;595(2):206-219. doi: 10.1002/1873-3468.14001. 
            Epub 2020 Dec 3.
  PUBMED    33210733
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.14001"; journalName: "FEBS Lett"; date: 
            "2021-01"; volume: "595"; issue: "2"; pages: "206-219"
COMMENT     Sequence Label: pBAD-wtBlc
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..99
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(642..659)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      812..898
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gacgcttttt atcgcaactc tctactgttt ctccataccc gttttttggg ctaacaggag
       61 gaattaacca tggggggttc tcatcatcat catcatcatc tcgaatcaac cagcttgtac
      121 aagaaatcca gctccacgcc gccgcgtggc gtgaccgtag taaataattt cgacgccaaa
      181 cgttatctcg gtacctggta tgagattgcc cgttttgatc accgctttga acgtggactg
      241 gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa
      301 ggctataacc ctgacagagg aatgtggcag cagagtgaag ggaaagcgta ctttaccggc
      361 gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac
      421 gttattgcac tcgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac
      481 ctgtggatac tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca
      541 gtcgcgaccc gggaagggtt tgatgtcagt aaatttattt gggtacagca gcctggtagt
      601 tagaagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
      661 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
      721 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
      781 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      901 gccgggagcg gatttgaacg ttgcgaagca acggccc
//
