LOCUS       sequence-326219-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-fragment. This plasmid can be co-transformed with either Plasmid 
            168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to 
            obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bozhanova NG, Calcutt MW, Beavers WN, Brown BP, Skaar EP, Meiler J
  TITLE     Lipocalin Blc is a potential heme-binding protein.
  JOURNAL   FEBS Lett. 2021 Jan;595(2):206-219. doi: 10.1002/1873-3468.14001. 
            Epub 2020 Dec 3.
  PUBMED    33210733
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.14001"; journalName: "FEBS Lett"; date: 
            "2021-01"; volume: "595"; issue: "2"; pages: "206-219"
COMMENT     Sequence Label: pMRBad-Z-C-wtBlc
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             372..394
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(421..705)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(479..498)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tctttcgggc tttgttagca gccggatcct tagttgtaca ctaactacca ggctgctgta
       61 cccaaataaa tttactgaca tcaaaccctt cccgggtagc gactgccagc atctcctgtt
      121 tcacttcgtc agaaatggtt ggcgtgcggg agagtatcca caggtagtcg cggtccgggc
      181 cgcaaaccag cgcatggcgg tattcccgat cgagtgcaat aacgttataa ccgccataga
      241 aaggaccacc gcttccaccc gacgtctgcg cgagtttttt ttccaatgct tggtttttcc
      301 attccagctg agcaagtttt ttctccaggg cttgtaactt cttttccagc tgctcgcttg
      361 ccatgggtat atctccttct taaagttaaa caaaattatt tctagcccaa aaaaacgggt
      421 atggagaaac agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg
      481 ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc
      541 tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt
      601 acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga
      661 cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga
      721 gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg
      781 cccatctggt ggcgggttta acgccgattg
//
