LOCUS       sequence-326223-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-fragment. This plasmid can be co-transformed with either Plasmid 
            168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain 
            wtBlc‐split or DiB2‐split protein, correspondingly .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Bozhanova NG, Calcutt MW, Beavers WN, Brown BP, Skaar EP, Meiler J
  TITLE     Lipocalin Blc is a potential heme-binding protein.
  JOURNAL   FEBS Lett. 2021 Jan;595(2):206-219. doi: 10.1002/1873-3468.14001. 
            Epub 2020 Dec 3.
  PUBMED    33210733
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.14001"; journalName: "FEBS Lett"; date: 
            "2021-01"; volume: "595"; issue: "2"; pages: "206-219"
COMMENT     Sequence Label: pMRBad-C-wtBlc
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             258..280
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(307..591)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(365..384)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tctttcgggc tttgttagca gccggatcct tagttgtaca ctaactacca ggctgctgta
       61 cccaaataaa tttactgaca tcaaaccctt cccgggtagc gactgccagc atctcctgtt
      121 tcacttcgtc agaaatggtt ggcgtgcggg agagtatcca caggtagtcg cggtccgggc
      181 cgcaaaccag cgcatggcgg tattcccgat cgagtgcaat aacgttataa ccgccataga
      241 aaggacccat gggtatatct ccttcttaaa gttaaacaaa attatttcta gcccaaaaaa
      301 acgggtatgg agaaacagta gagagttgcg ataaaaagcg tcaggtagga tccgctaatc
      361 ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa tcaatgtgga
      421 cttttctgcc gtgattatag acacttttgt tacgcgtttt tgtcatggct ttggtcccgc
      481 tttgttacag aatgctttta ataagcgggg ttaccggttt ggttagcgag aagagccagt
      541 aaaagacgca gtgacggcaa tgtctgatgc aatatggaca attggtttct tctctgaatg
      601 gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt
      661 ttaatgccca tctggtggcg ggtttaacgc cgattgaggc caacggttat ctcgattttt
      721 ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt cgcggtcagg
      781 gggtggtgaa aaatcaggga cgagaatttg tttgccgacc gggtgatatt ttgctgttcc
      841 cgccaggaga gattcatcac tacggtcgtc atccggaggc tcgcgaatgg
//
