LOCUS       sequence-326267-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Cao F, Zhou Y, Liu X, Yu CH
  TITLE     Podosome formation promotes plasma membrane invagination and 
            integrin-beta3 endocytosis on a viscous RGD-membrane.
  JOURNAL   Commun Biol. 2020 Mar 13;3(1):117. doi: 10.1038/s42003-020-0843-2.
  PUBMED    32170110
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-03-13"; volume: "3(1):117. doi"; pages: " 
            10.1038/s42003-020-0843-2"
COMMENT     Sequence Label: DNM2 delta PRD
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    549..601
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      609..618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(660..681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(921..940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcatacctcc tctggaacct cagatccgtg atgtggagaa gggcttcatg tccaacaagc
       61 acgtcttcgc catcttcaac acggagcaga gaaacgtcta caaggacctg cggcagatcg
      121 agctggcctg tgactcccag gaagacgtgg acagctggaa ggcctcgttc ctccgagctg
      181 gcgtctaccc cgagaaggac caggcagaaa acgaggatgg ggcccaggag aacaccttct
      241 ccatggaccc ccaactggag cggcaggtgg agaccattcg caacctggtg gactcatacg
      301 tggccatcat caacaagtcc atccgcgacc tcatgccaaa gaccatcatg cacctcatga
      361 tcaacaatac gaaggccttc atccaccacg agctgctggc ctacctatac tcctcggcag
      421 accagagcag cctcatggag gagtcggctg accaggcaca gcggcgggac gacatgctgc
      481 gcatgtacca tgccctcaag gaggcgctca acatcatcgg tgacatcagc accagcactg
      541 tgtccacgct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      601 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      661 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      721 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      781 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      841 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaagc tacgtccagg
      901 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      961 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1021 acatcctggg gcacaagctg gagtacacta cacagccaca acgtctatat catggagaca
     1081 gcagagacgg catcagtgac ttcagatcgc acacatcgag acgcagcgtg cagctcgcga
     1141 cactacagca gacacccctc atcggcgacg accgtgcctg cctgcccgac accctactga
     1201 cacagttcga cttgaccaga cccacgaaag cgcgaatcac atggtccctg ctcttgggat
//
