LOCUS       sequence-326268-        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Cao F, Zhou Y, Liu X, Yu CH
  TITLE     Podosome formation promotes plasma membrane invagination and 
            integrin-beta3 endocytosis on a viscous RGD-membrane.
  JOURNAL   Commun Biol. 2020 Mar 13;3(1):117. doi: 10.1038/s42003-020-0843-2.
  PUBMED    32170110
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-03-13"; volume: "3(1):117. doi"; pages: " 
            10.1038/s42003-020-0843-2"
COMMENT     Sequence Label: Bin1 N-BAR domain
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    852..904
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      912..921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccatgtggag ttatagcaga gctggtttag tgaccgtcag atccgctagc atggcagaga
       61 tggggagcaa gggggtgacg gcggggaaga tcgccagcaa cgtacagaag aagctgaccc
      121 gagcgcagga gaaggtcctg cagaaactgg ggaaggcgga cgagacgaag gacgagcagt
      181 ttgagcagtg tgtccagaac ttcaataagc agctgacaga gggtacccgg ctgcagaagg
      241 atcttcggac ctatctggct tctgttaaag cgatgcacga agcctccaag aagctgagtg
      301 agtgtcttca ggaggtgtat gagcccgagt ggcctggcag ggatgaagca aacaagattg
      361 cagagaacaa tgacctactc tggatggact accaccagaa gctggtggac caggctctgc
      421 tgaccatgga cacctaccta ggccagttcc ctgatatcaa gtcgcgcatt gccaagcggg
      481 ggcggaagct ggtggactat gacagtgccc ggcaccacta tgagtctctt caaaccgcca
      541 aaaagaagga tgaagccaaa attgccaagg cagaagagga gctcatcaaa gcccagaagg
      601 tgttcgagga gatgaacgtg gatctgcagg aggagctgcc atccctgtgg aacagccgtg
      661 taggtttcta tgtcaacacg ttccagagca tcgcgggtct ggaggaaaac ttccataaag
      721 agatgagtaa gctcaatcag aacctcaatg atgtcctggt cagcctagag aagcagcacg
      781 ggagcaacac cttcacagtc aaggcccaac ccagtgacaa tgcccctgag aaagggaaca
      841 agagcccgtc actcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
      901 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgcccg
      961 tcctgctcga gctggacggc gacgtaaacg gcacaagttc agcgtgtcag caaggcgagg
     1021 gcgatgccat ctacggcaag ctgaccctga ggttcatctg caccactggt aagctgctcg
     1081 atgccctggc ccacctcatg acgacgtgta cctacagtag ccagtggttt catctgctac
     1141 ccgaaacaga tgatcaacac acttcgtgag tccgccatgt ccgaaggcaa cgtccgggcg
     1201 ttcgatcttt tttaggaaga cagggcacta cagacacctc gtccgaagcg tatatgtgt
//
