LOCUS       sequence-326272-        1294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Cao F, Zhou Y, Liu X, Yu CH
  TITLE     Podosome formation promotes plasma membrane invagination and 
            integrin-beta3 endocytosis on a viscous RGD-membrane.
  JOURNAL   Commun Biol. 2020 Mar 13;3(1):117. doi: 10.1038/s42003-020-0843-2.
  PUBMED    32170110
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-03-13"; volume: "3(1):117. doi"; pages: " 
            10.1038/s42003-020-0843-2"
COMMENT     Sequence Label: Bin1 delta SH3
FEATURES             Location/Qualifiers
     source          1..1294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    375..427
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      435..444
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(441..443,444..446,447..959)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(486..507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(747..766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaccgccggt gacatgagca gagccggcca gtggggcctc acccggggct accatcccca
       61 agtccccatc tcagctccgg aaaggcccac ctgtccctcc gcctcccaaa cacaccccat
      121 ccaaggagat gaagcaggag cagattctca gcctttttga tgacgcattt gtccctgaga
      181 tcagcgtgac caccccctcc cagccagcag aggcctccga ggtggtgggt ggagcccagg
      241 agccagggga gacagcagcc agtgaagcaa cctccagctc tcttccggct gtggtggtgg
      301 agaccttctc cgcaactgtg aatggggcgg tggagggcag cgctgggact ggacgcttgg
      361 acctgccccc gggactcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc
      421 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      481 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      541 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      601 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      661 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      721 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      781 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      841 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      901 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      961 acggcagcgt gcagctcgcc gaccactacc agcagacacc cccatcggcg acggccccgt
     1021 gctgctgccc gacaccacta cctgagcacc cagtccgccc tgagcaagac ccacgagaag
     1081 cgcgatcaca tggtctgctg agttcgtgac gcgcgggatc actctcggca tgacgagctg
     1141 tacaagtaag cggccgccga cctctagatc atatcagcat accacatttg taaaggttac
     1201 tgctttaaaa cctcccaacc ttcccttgac ctggaactta atgatgccat tgttgttgtt
     1261 ttaaccgtgt ttatgtccag ccttatatag aggg
//
