LOCUS       sequence-32628-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Held M, Schmitz MH, Fischer B, Walter T, Neumann B, Olma MH, Peter 
            M, Ellenberg J, Gerlich DW
  TITLE     CellCognition: time-resolved phenotype annotation in high-throughput
            live cell imaging.
  JOURNAL   Nat Methods. 2010 Sep . 7(9):747-54.
  PUBMED    20693996
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Sep . 7(9):747-54."
COMMENT     Sequence Label: pPDS1-mEGFP-IRES-puro2b
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(730..781)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(759..779)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggcgacc ggtggatccc gaatatctat gtcacagcaa
       61 acaggtggca attcaacatc cagggtcgac agaatgcttg aaggagactg caacagattg
      121 gattcccatg gtggagaggg catcttcaca ggtgaagggg ggcccagctg aaacagcttt
      181 tcaagctctc tctcctcgtc aaggatcatg agaggcactc cactcaaggg gaggtgcgca
      241 atctggtgct cttcaggcag gtcaaaactc tcaaagtcta gaggattgaa gggaaagaat
      301 ttttctattt ctggataggc atcatctgag gcaggaacag agctttttgc tttaacagtc
      361 ttctcagtca tctttttggc agaaaagctt ggctgttttt gtttgagggg tcccttggtc
      421 tttacagact tttctgtagc tctgttgaca gttcccaaag cctttctagt agctttaggt
      481 aaggctggtg gggcatcgaa cgttttgcca aaacgtggtg ttgaaacttg agatctccca
      541 tctaaggctt tgattgaagg tccagacccc agcttcagcc catccttagc aaccacacgg
      601 gtgcctggtt ctccattttc cttatcaaca tagatcagag tagccatggt accgtcgact
      661 gcagaattcg aagcttgagc tcgagatctg agtccggtag cgctagcgga tctgacggtt
      721 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      781 caatggggcg ga
//
