LOCUS       sequence-326294-        4059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks 
            physical marker or attB site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pHSP70B-CP6-TK
FEATURES             Location/Qualifiers
     source          1..4059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..452
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     400..421
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     rep_origin      complement(1844..2432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1924..1943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2547..2649
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2650..3465
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2650..3465
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2723..2742)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3579..4034
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3666..3685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3876..3897
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctagaatccc aaaacaaact ggttattgtg gtaggtcatt tgtttggcag aaagaaaact
       61 cgagaaattt ctctggccgt tattcgttat tctctctttt ctttttgggt cgctccctct
      121 ctgcactaat gctctctcac tctgtcacac agtaaacggc atactgctct cgttggttcg
      181 agagagcgcg cctcgaatgt tcgcgaaaag agcgccggag tataaataga ggcgcttcgt
      241 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
      301 gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt
      361 gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa
      421 gaagtaatta ttgaatacaa gaagagaact ctcaatactc gaatccatgt gggagtttat
      481 tcttgacaca gatatttatg atataataac tgagtaagct taacataagg aggaaaacaa
      541 aatggccagc tacccctgcc atcagcacgc cagcgccttc gatcaggctg cccgcagccg
      601 cggacacaac aaccgtcgca ccgccctgcg cccacgccgc cagcagaagg ccaccgaagt
      661 ccgcttggag cagaagatgc ccacactgct gcgcgtgtac atcgatggcc cccatggcat
      721 gggcaagacc accacgaccc aactgctggt cgccctgggc agccgcgacg acatcgtcta
      781 cgtgcccgag cccatgacct actggcaagt cctgggcgcc tccgagacaa ttgccaacat
      841 ctacaccacc cagcaccgct tggatcaggg cgagatcagt gccggcgacg ccgccgtggt
      901 gatgaccagc gcccagatca ccatgggaat gccctacgcc gtgaccgatg ccgtcctggc
      961 cccccacatt ggaggcgagg ccggcagctc gcatgcccca cccccagccc tgacaatctt
     1021 cctggatcgc cacccgatcg ccttcatgct gtgctaccca gccgcccgtt atctgatggg
     1081 cagcatgacc ccccaggccg tgctggcctt cgtggccctg atccccccga ccctgcccgg
     1141 caccaacatc gtgctgggag ccctgcccga ggatcgccac atcgaccgcc tggccaagcg
     1201 ccagcgtcca ggagagcgcc tggatctggc catgctggcc gccatccgcc gcgtgtacgg
     1261 cctgctggcc aacaccgtgc gctatcttca aggaggcggc tcctggcgcg aggattgggg
     1321 ccaactgtcc ggaacggccg tgccacccca gggagccgag ccccagagca atgccggccc
     1381 acgcccccac atcggcgata ccctgtttac gctgttccgt gcccccgagc tgctggcccc
     1441 gaacggcgat ttgtacaatg tgttcgcctg ggccctggat gtgctggcga agcgtctgcg
     1501 ccccatgcac gtgttcatcc tggattacga tcaaagccca gccggatgcc gcgacgccct
     1561 cttgcaattg acaagcggca tggtgcagac ccatgtgacc acccccggca gcatccccac
     1621 aatctgcgat ctggcccgca cattcgcccg cgagatgggc gaggctaatt aagcttggca
     1681 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttccgcttg agacgaagct
     1741 tgacacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2281 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2521 ggatcttcac ctagatcctt ttgtgatgat cggcacgtaa gaggttccaa ctttcaccat
     2581 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     2641 gaagctaaaa tgagccatat tcaacgggaa acgtcttgct caaggccgcg attaaattcc
     2701 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2761 gcgacaatct accgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2821 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2881 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2941 accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt
     3001 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     3061 aattgtcctt ttaacagcga tcgcgtattt cgactcgctc aggcgcaatc acgaatgaat
     3121 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     3181 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtagt cactcatggt
     3241 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     3301 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     3361 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3421 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctgattttt tttaaggcag
     3481 ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa
     3541 tggcagaaat tcgatgataa gctgtcaaac atgatgttac gcgccctgta gcggcgcatt
     3601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3781 caaaaaactt gattagggtg atggttcacg tagtgggcca gcgccctgat agacggtttt
     3841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4021 aacgcttaca attttaacaa gcttcgtctc agtcaggag
//
