LOCUS       sequence-32630-e         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting 
            sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Pierce AJ, Hu P, Han M, Ellis N, Jasin M
  TITLE     Ku DNA end-binding protein modulates homologous repair of 
            double-strand breaks in mammalian cells.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3237-42.
  PUBMED    11751629
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3237-42."
COMMENT     Sequence Label: pHPRT-DRGFP
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..29
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagcaccacc aaaaaaaaaa cgaaaagtag aagacccacg aggcaacacc agcggcgtgc
       61 tgagcacccc caaggccaag agggccaagc acccccccgg caccgagaag cccaggagca
      121 ggagccagag cgagcagccc gccacctgcc ccatctgcta cgccgtgatc aggcagagca
      181 ggaacctgag gaggcacctg gagctgaggc acttcgccaa gcccggcgtg gatccaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggctaggga taacagggta
      361 atacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ctgacctacg g
//
