LOCUS       sequence-326309-        8071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. 
            Contains an Alpha level GB2.0 entry point for addition of custom 
            assemblies utilizing blue-white bacterial colony screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pBlastR-5xUAS-sfGFP-GMRWhite
FEATURES             Location/Qualifiers
     source          1..8071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2499..2633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3790..3924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4093..4681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4173..4192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5149..5940
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5995..6450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6082..6101)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6292..6313
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6474..6521
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6575..6593
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6575..6592
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    6600..6656
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6658..6677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6659..6677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6684..6701)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6684..6700)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6693..6715)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6909..6928)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    6985..7054
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     7481..7502
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 acgcacgggt gttgggtcgt ttgttccgct gtcaggagcg tacgactagc acctgactgt
       61 cgagcccagt ggaaaccctt gaaatgcctt taagtcgagc ccagtggaaa cccttgaaat
      121 gcctttaagt cgagcccagt ggaaaccctt gaaatgcctt taagtcgagc ccagtggaaa
      181 cccttgaaat gcctttaagt cgagcccagt ggaaaccctt gaaatgcctt taagtcgagt
      241 agcagtcgag gtaggtacgt catgcccctc ccgagctcgc ccggggatcg agcgcagcgg
      301 tataaaaggg cgcggggtgg ctgagagcat cagttgtgaa tgaatgttcg agccgagcag
      361 acgtgccgct gccttcgtta atatcctttg aataagccaa ctttgaatca caagacgcat
      421 accaaaccaa atgggacagg aggaccagga gctgctgatc cgcggaggct ccaagcaccc
      481 aagcgccgag cacctgaaca acggcgatag cggagccgcc agccagtcgt gcatcaatca
      541 gggatttggc caggccaaga attacggaac cctgctgccc cccagccccc cagaggatag
      601 cggaagcgga tccggacagc tggccgagaa tctgacgtac gcctggcata acatggatat
      661 cttcggcgcc gtgaaccagc caggcagtgg ctggcgtcag ctggtgaacc gcacccgcgg
      721 actgttctgc aatgagcgcc atatcccagc cccacgcaag cacctgctga agaatgtgtg
      781 cggagtggcc taccccggcg agctgctggc cgtgatggga agtagtggcg ccggaaagac
      841 cacgctgctg aacgccctgg ccttccgcag cccccagggc attcaggtgt ccccaagcgg
      901 catgcgcctg ctgaacggcc agcccgtgga tgccaaggag atgcaggccc gctgcgccta
      961 cgtgcagcag gacgatctgt tcatcggcag cctgacagcc cgcgagcatc tgatctttca
     1021 ggccatggtc cgcatgcccc gccacctgac ataccgtcag cgcgtggccc gcgtggacca
     1081 ggtcatccag gagctgagcc tgagcaagtg ccagcacacc attattggcg tgccaggccg
     1141 cgtgaagggc ctgagtggcg gagagcgcaa gcgcctggcc tttgccagcg aggccctgac
     1201 cgatcccccc ctgctgattt gcgacgagcc gaccagcggc ctggatagct tcaccgccca
     1261 ctccgtggtg caggtgctga agaagctgag ccagaaggga aagacggtca ttctgacgat
     1321 ccaccagccc agctcggagc tgttcgagct gtttgataag attctgctga tggccgaggg
     1381 acgcgtggcc ttcctgggaa ccccaagcga ggccgtggat ttcttcagct atgtgggcgc
     1441 ccagtgcccc acgaattaca accccgccga cttctatgtg caagtgctgg ccgtggtgcc
     1501 aggacgcgag attgagagcc gcgatcgcat tgccaagatc tgcgataact tcgccatctc
     1561 caaggtggcc cgcgacatgg agcagctgct ggccacgaag aacctggaga agcccctgga
     1621 gcagcccgag aacggctata cgtataaggc tacctggttt atgcagtttc gcgccgtgct
     1681 gtggcgcagc tggctgagtg tgctgaagga gcccctgctg gtcaaggtcc gcctgatcca
     1741 gaccacgatg gtggccattc tgatcggcct gatcttcctg ggacagcagc tgacgcaggt
     1801 cggagtgatg aacatcaatg gagccatttt tctgtttctg acgaacatga cgttccagaa
     1861 cgtgttcgcc accatcaacg tgttcaccag cgagctgccc gtgttcatgc gcgaggcccg
     1921 cagccgcctg taccgctgcg atacctactt cctgggaaag accattgccg agctgccgct
     1981 gttcctgacc gtgcccctgg tgtttaccgc cattgcctac cccatgattg gcctgcgtgc
     2041 cggagtgctg catttcttta attgcctggc cctggtgacc ctggtggcca acgtgtcgac
     2101 cagcttcggc tacctgatca gctgcgcctc cagcagcacc agcatggccc tgagcgtggg
     2161 accccccgtg attatcccct tcctgctgtt cggcggattc tttctgaact ccggcagcgt
     2221 gcccgtgtac ctgaagtggc tgagctacct gagctggttt cgctacgcca atgagggcct
     2281 gctgatcaat cagtgggccg atgtggagcc cggcgagatc tcctgcacca gcagcaacac
     2341 gacctgcccc agcagcggaa aggtcatcct ggagacactg aatttcagtg ccgccgacct
     2401 gccactggat tatgtgggcc tggccatcct gattgtgtcc ttccgcgtgc tggcctatct
     2461 ggctctgcgc ctgcgcgctc gtcgcaagga gtgagcttga tccagacatg ataagataca
     2521 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2581 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2641 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcagtat
     2701 ggcaagcgct cgacgaatta acaagcttcg ctctatctcg ctctctatct ctctcgatcg
     2761 ctcgtgctaa gcttaagacc gtggagaaca aggagaaccg ctgtcaggag cggagtactg
     2821 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     2881 tccgagcgga gtactgtcct ccgtcccgag ctcgcccggg gatcgagcgc agcggtataa
     2941 aagggcgcgg ggtggctgag agcatcagtt gtgaatgaat gttcgagccg agcagacgtg
     3001 ccgctgcctt cgttaatatc ctttgaataa gccaactttg aatcacaaga cgcataccaa
     3061 accaaatggt gtccaagggc gaggagctgt tcaccggcgt ggtgcccatc ctggtggagc
     3121 tggatggcga cgtgaacggc cacaagttca gcgtgcgcgg cgagggcgag ggcgacgcca
     3181 ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3241 ccaccctggt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgatcaca
     3301 tgaagcagca cgatttcttc aagagcgcca tgcccgaggg ctacgtgcag gagcgcacca
     3361 tcagcttcaa ggatgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgata
     3421 ccctggtgaa ccgcatcgag ctgaagggca tcgatttcaa ggaggatggc aacatcctgg
     3481 gccacaagct ggagtacaac ttcaacagcc acaacgtgta catcaccgcc gataagcaga
     3541 agaacggcat caaggccaac ttcaagatcc gccacaatgt ggaggatggc tccgtgcagc
     3601 tggccgatca ctaccagcag aacaccccca tcggcgacgg cccagtgctg ctgcccgata
     3661 accactacct gagcacccag agcgtgctgt ccaaggaccc caacgagaag cgcgatcaca
     3721 tggtgctgct ggagttcgtg accgccgccg gcatcaccct gggcatggat gagctgtaca
     3781 agtaagcttg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3841 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3901 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3961 cagggggagg tgtgggaggt tttttcgctt gacacggtta tccacagaat caggggataa
     4021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgtgaccagc
     4801 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     4861 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     4921 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     4981 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     5041 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     5101 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg
     5161 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     5221 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     5281 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     5341 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     5401 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     5461 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     5521 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     5581 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     5641 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     5701 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     5761 aaggatgtag ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     5821 cttgaagcta gacaggctta tcttggacaa gaagaagatc gtttggcctc gcgcgcagat
     5881 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     5941 tcactagacc aatgttacac atatatactt tagattgatt taaaacttca tgttacgcgc
     6001 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6061 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6121 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6181 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccagcgc
     6241 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6301 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6361 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6421 attttaacaa aatattaacg cttacaattt taacggatca gcgagagacc cctgttgaca
     6481 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaaccc
     6541 aggaggcaga tcatgaccat gattacgcca agctatttag gtgacactat agaatactca
     6601 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaattcgccc
     6661 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6721 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6781 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatga
     6841 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     6901 actctcagta caatctgctc tgatgccgca tagggtctct ctccggatcc cagggaggaa
     6961 ttggtcgacg atgtaggtca ctgtctcgaa gccgcggtgc gggtgccagg gcgtgccctt
     7021 gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatgc gctgtcagga
     7081 gctagaatcc caaaacaaac tggttattgt ggtaggtcat ttgtttggca gaaagaaaac
     7141 tcgagaaatt tctctggccg ttattcgtta ttctctcttt tctttttggg tcgctccctc
     7201 tctgcactaa tgctctctca ctctgtcaca cagtaaacgg catactgctc tcgttggttc
     7261 gagagagcgc gcctcgaatg ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg
     7321 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
     7381 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag
     7441 tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca
     7501 agaagtaatt attgaataca agaagagaac actcaatact cgaatccatg tgggagttta
     7561 ttcttgacac agatatttat gatataataa ctgagtaagc ttaacataag gaggaaaaca
     7621 aaatgaaaac cttcaacatc agccagcagg acctggaact ggtggaggtg gctaccgaga
     7681 agattacgat gctctacgag gataacaagc accacgtggg agctgccatt cgcaccaaga
     7741 cgggcgagat tatcagtgcc gtgcacattg aggcctacat cggacgcgtg accgtgtgtg
     7801 ccgaggccat tgccattggc agtgccgtga gcaacggcca gaaggatttt gataccattg
     7861 tggccgtgcg tcacccatac tccgatgagg tggaccgtag catccgtgtc gtgtccccgt
     7921 gtggaatgtg ccgtgagctg attagcgatt acgcccccga ttgcttcgtg ctgattgaga
     7981 tgaacggcaa gctggtcaag accaccattg aggaactgat tcccctgaag tatacccgta
     8041 actaagcttg gcaataaaaa gacagaataa a
//
