LOCUS       sequence-326311-       10297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistant QF driver vector with Mini-white CDS eye 
            marker. Contains an enhancer grammar GB20 entry point for custom 
            enhancers. Standard cut-and-paste cloning can also be used. Vector 
            uses Purple-White bacteria colony screening for identification of 
            correct cloning .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pBlastR-MCS-QF-MiniWhite
FEATURES             Location/Qualifiers
     source          1..10297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    355..489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(379..398)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     433..452
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(577..595)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1047..1118,1520..1793,1868..2522,2584..2899,3103..3234,
                     3305..3919)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     rep_origin      complement(4686..5274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4766..4785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5742..6533
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6588..7043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6675..6694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6885..6906
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    7166..7235
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     7662..7683
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        8291..8309
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8291..8308
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8320..8337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      9145..9239
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        complement(9363..9381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccatcaccca gaccaacgac tacggttccg tcaataccca cggcatcttg tccacttacc
       61 ccccgccagc cacccacctc aacgaggcct ccgtcgccct ggctcccggc ggagctccac
      121 cccgtccccc gcccccctac gtcgactcca ccaccaacca cccgccctac cactccaacc
      181 tggtgcccat ggctaacttc ggatactcca ccgttgacta cgatgccatg gtggacgatt
      241 tggcttccat cgagtacact gacgccgttg acgtcgatcc gcagttcatg accaacctgg
      301 gtttcgttcc cggctgcaac ttctccgaca tcaacactta cgagcagtaa gcttgatcca
      361 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      421 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      481 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      541 gaggtttttt cgctgtcagg aggaattcta gtatgtatgt aagttaataa aacccatttt
      601 tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt
      661 atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga
      721 ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt
      781 ccacagaaat atcgccgtca ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg
      841 tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga
      901 agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc
      961 ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc
     1021 aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg
     1081 gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca
     1141 tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga
     1201 gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca
     1261 gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa
     1321 gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga
     1381 tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg
     1441 atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     1501 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     1561 cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg
     1621 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     1681 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     1741 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     1801 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     1861 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     1921 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     1981 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     2041 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     2101 ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     2161 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     2221 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     2281 gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc
     2341 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     2401 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     2461 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     2521 tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc
     2581 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     2641 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     2701 ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga
     2761 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     2821 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     2881 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     2941 ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag
     3001 tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac
     3061 tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     3121 ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc
     3181 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     3241 catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat
     3301 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     3361 ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc
     3421 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     3481 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     3541 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     3601 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     3661 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     3721 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     3781 gggcaaggtc atcctggaga cacttaactt ctccgccgcc gatctgccgc tggactacgt
     3841 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     3901 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac
     3961 cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt
     4021 gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc
     4081 acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat
     4141 caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata catacttttc
     4201 aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc
     4261 gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac
     4321 tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcggctc
     4381 aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata
     4441 tatttccctc acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt
     4501 tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg
     4561 ttccttgtgt agatgcatcg cttgacacgg ttatccacag aatcagggga taacgcagga
     4621 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4681 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4741 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4801 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4861 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4921 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4981 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5041 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5101 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5161 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5221 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5281 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5341 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgtgacc agccaggaca
     5401 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     5461 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     5521 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     5581 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     5641 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     5701 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     5761 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     5821 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     5881 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     5941 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     6001 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     6061 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     6121 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     6181 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     6241 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     6301 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     6361 tagctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     6421 ctagacaggc ttatcttgga caagaagaag atcgtttggc ctcgcgcgca gatcagttgg
     6481 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatcactag
     6541 accaatgtta cacatatata ctttagattg atttaaaact tcatgttacg cgccctgtag
     6601 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6661 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6721 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6781 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccag cgccctgata
     6841 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6901 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6961 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7021 caaaatatta acgcttacaa ttttaacgga tcagcgcagc tgcccgggtc tagagtcgac
     7081 gcatgcctcc tgacagcgag atgtcctaat atggacatcc tctccggatc ccagggagga
     7141 attggtcgac gatgtaggtc actgtctcga agccgcggtg cgggtgccag ggcgtgccct
     7201 tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg cgctgtcagg
     7261 agctagaatc ccaaaacaaa ctggttattg tggtaggtca tttgtttggc agaaagaaaa
     7321 ctcgagaaat ttctctggcc gttattcgtt attctctctt ttctttttgg gtcgctccct
     7381 ctctgcacta atgctctctc actctgtcac acagtaaacg gcatactgct ctcgttggtt
     7441 cgagagagcg cgcctcgaat gttcgcgaaa agagcgccgg agtataaata gaggcgcttc
     7501 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
     7561 aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa
     7621 gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc
     7681 aagaagtaat tattgaatac aagaagagaa cactcaatac tcgaatccat gtgggagttt
     7741 attcttgaca cagatattta tgatataata actgagtaag cttaacataa ggaggaaaac
     7801 aaaatgaaaa ccttcaacat cagccagcag gacctggaac tggtggaggt ggctaccgag
     7861 aagattacga tgctctacga ggataacaag caccacgtgg gagctgccat tcgcaccaag
     7921 acgggcgaga ttatcagtgc cgtgcacatt gaggcctaca tcggacgcgt gaccgtgtgt
     7981 gccgaggcca ttgccattgg cagtgccgtg agcaacggcc agaaggattt tgataccatt
     8041 gtggccgtgc gtcacccata ctccgatgag gtggaccgta gcatccgtgt cgtgtccccg
     8101 tgtggaatgt gccgtgagct gattagcgat tacgcccccg attgcttcgt gctgattgag
     8161 atgaacggca agctggtcaa gaccaccatt gaggaactga ttcccctgaa gtatacccgt
     8221 aactaagctt ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttccg
     8281 ctgtcaggag atttaggtga cactatagac ttccgcttcc tcgctcactg actcgctgcg
     8341 ctcggtcgtt cggctgtcgg gagagagacc aatccatgtg ggagtttatt cttgacacag
     8401 atatttatga tataataact gagtaagctt aacataagga ggaaaacaaa atggcgagct
     8461 tggttaagaa agatatgtgt gttaagatga cgatggaagg tactgtgaac ggttatcact
     8521 ttaagtgcgt tggcgagggt gaaggcaagc cgttcgaggg cacgcagaac atgcgcattc
     8581 gtgtcaccga gggcggtccg ctgccttttg cattcgacat cctggccccg tgctgtatgt
     8641 acggctctaa gaccttcatt aaacacgtga gcggtatccc ggattacttt aaagagtcct
     8701 ttccagaggg cttcacttgg gaacgtaccc agatttttga ggacggtggt gttctgaccg
     8761 cgcaccaaga caccagcctg gaaggtaatt gcctgatcta taaagtgaag gttctgggta
     8821 ccaatttccc ggcgaatggt ccggtgatgc aaaagaaaac cgcgggttgg gagccgtgcg
     8881 tcgagatgct gtatccgcgt gacggcgtct tgtgtggtca gagcttgatg gcgctgaagt
     8941 gcaccgatgg caatcatctg accagccacc tgcgcacgac gtatcgtagc cgtaaaccga
     9001 gcaacgccgt taacatgccg gagttccatt ttggtgacca tcgcatcgaa atcctgaaag
     9061 ctgagcaggg caaattctac gaacaatacg aatcggctgt cgcacgttac agcgatgtgc
     9121 cggaaaaagc gacgtaatga gcttgactcc tgttgataga tccagtaatg acctcagaac
     9181 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatt
     9241 acttcaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat
     9301 cggtctcttc ccgcttgtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     9361 ggcctatagt gagtcgtatt atcccgagct cgcccgggga tcgagcgcag cggtataaaa
     9421 gggcgcgggg tggctgagag catcagttgt gaatgaatgt tcgagccgag cagacgtgcc
     9481 gctgccttcg ttaatatcct ttgaataagc caactttgaa tcacaagacg cataccaaac
     9541 caaatgcccc ccaagcgcaa gactctgaac gccgccgctg aggccaacgc ccacgctgac
     9601 ggtcatgctg atggtaacgc tgatggtcac gtcgccaata ccgctgctag ctccaacaac
     9661 gcccgtttcg ccgacctgac caatattgat acccccggct tgggccccac caccaccacc
     9721 ctgctggtgg agcccgcccg ttccaagcgt cagcgtgtgt cccgcgcctg cgaccagtgc
     9781 cgcgccgctc gtgagaagtg cgacggaatc cagccagcct gcttcccctg cgtgtcccag
     9841 ggccgctcct gcacttacca ggcctcccca aaaaagcgcg gcgtgcagac tggatacatt
     9901 cgcaccctcg agctcgccct ggcctggatg ttcgagaacg tggcccgctc cgaggatgct
     9961 ctgcataacc tgttggtccg tgacgccgga cagggctcgg ccctgctggt cggcaaggac
    10021 tcgcccgccg ctgagcgcct gcatgctcgt tgggccacca gccgtgtcaa caagagcatc
    10081 acccgcctcc tgagtggcgg aggttccggt ggcggcagcg gcgggggttc gggaggtggc
    10141 tcgcgccagc tggagctgcc acccactgcc accgccaccg cttcgattat gccacacgtc
    10201 atggagcagc cgctgtccac ctccatcaac cccgtcaacg atcgcttcaa cggcatccca
    10261 aaccccaccc cctacaactc cgacgccgcc ctggacg
//
