LOCUS       sequence-326328-        3576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA targeting the yellow gene in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Kandul NP, Belikoff EJ, Liu J, Buchman A, Li F, Yamamoto A, Yang T, 
            Shriner I, Scott MJ, Akbari OS
  TITLE     Genetically Encoded CRISPR Components Yield Efficient Gene Editing 
            in the Invasive Pest Drosophila suzukii.
  JOURNAL   CRISPR J. 2021 Oct;4(5):739-751. doi: 10.1089/crispr.2021.0032.
  PUBMED    34661429
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2021"; journalName: "CRISPR J"; date: "2021-10"; 
            volume: "4"; issue: "5"; pages: "739-751"
COMMENT     Sequence Label: gRNAy
FEATURES             Location/Qualifiers
     source          1..3576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(293..295,296..298,299..1012)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(293..295,296..298,299..1009)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1135..1256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1290..1710
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        1731..1806
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(2389..2391,2392..2394,2395..3108)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3226..3347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3419..3518
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
ORIGIN
        1 ggttcccaca atggttaatt cgagctcgcc cggggatcta attcaattag agactaattc
       61 aattagagct aattcaatta ggatccaagc ttatcgattt cgaaccctcg accgccggag
      121 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
      181 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatcggg
      241 gtaccgctag agtcgacggt acccgcgggc ccgggatcca ccggtcgcca ccatggtgag
      301 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      361 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      421 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      481 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      541 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      601 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      661 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      721 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      781 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      841 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      901 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      961 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     1021 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     1081 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     1141 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1201 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaagc
     1261 ttatcgatac gcgtacggag cggcgcgcct tttttgctca cctgtgattg ctcctactca
     1321 aatacaaaaa catcaaattt tctgtcaata aagcatattt atttatattt attttacagg
     1381 aaagaattcc ttttaaagtg tattttaacc tataatgaaa aacgattaaa aaaaatacat
     1441 aaaataattc gaaaattttt gaatagccca ggttgataaa aattcatttc atacgtttta
     1501 taacttatgc ccctaagtat tttttgacca tagtgtttca attctacatt aattttacag
     1561 agtagaatga aacgccacct actcagccaa gaggcgaaaa ggttagctcg ccaagcagag
     1621 agggcgccag tgctcactac tttttataat tctcaacttc tttttccaga ctcagttcgt
     1681 atatatagac ctattttcaa tttaacgtcg ggtcctggac accggaaccg gttttagagc
     1741 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     1801 cggtgctttt ttcctactgc aggtaagttt tttgtttaaa ccatactcgg tggcctcccc
     1861 accaccaact tttttgcact gcaaaaaaac acgcttttgc acgcgggccc atacatagta
     1921 caaactctac gtttcgtaga ctattttaca taaatagtct acaccgttgt atacgctcca
     1981 aatacactac cacacattga acctttttgc agtgcaaaaa agtacgtgtc ggcagtcacg
     2041 taggccggcc ttatcgggtc gcgtcctgtc acgtacgaat cacattatcg gaccggacga
     2101 gtgttgtctt atcgtgacag gacgccagct tcctgtgttg ctaaccgcag ccggacgcaa
     2161 ctccttatcg gaacaggacg cgcctccata tcagccgcgc gttatctcat gcgcgtgacc
     2221 ggacacgagg cgcccgtccc gcttatcgcg cctataaata cagcccgcaa cgatctggta
     2281 aacacagttg aacagcatct gttacagcga cacaacatga gccggtccaa caacgccaac
     2341 gcgcccacgc catccaaccg ccgccgcaac ctgtctctgg tgctcgagat ggtgagcaag
     2401 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2461 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2521 ctgaagttca tctgcaccac cagcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2581 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2641 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2701 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2761 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2821 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     2881 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2941 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3001 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3061 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaacc gcgactctag
     3121 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     3181 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3241 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3301 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaaag cttgtactga
     3361 cggacacacc gaagccccgg cggcaaccct cagcggatgc cccggggctt cacgttttcc
     3421 caggtcagaa gcggttttcg ggagtagtgc cccaactggg gtaacctttg agttctctca
     3481 gttgggggcg tagggccgcc gacatgacac aaggggttgt gaccggggtg gacacgtacg
     3541 cgggtgctta cgaccgtcag tcgcgcgagc gcgacc
//
