LOCUS       sequence-326336-        9066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for GFP-hTREX1 delta C (aa1-235).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2445..2469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2538..2547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2544..2546,2547..2549,2550..3260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2544..2546,2547..2549,2550..3260)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2589..2610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2850..2869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3197..3218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3270..3294
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4013..4601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4066..4086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4484..4495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4652..4672)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4657..4986
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4837..4972
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5034..5081
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5100..5498
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5585..5818
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5890..6011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5927..5946)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5981..6000
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6051..6186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6206..6225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6207..6225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6235..6252)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6235..6251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6244..6266)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6393..6848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6480..6499)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6690..6711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6874..6978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6979..7047,7048..7839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7197..7216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8010..8598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8499..8518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8752..8769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8886..8907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8922..8927,8928..8945,8946..8952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8960..8976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8965..8987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8984..9000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8984..9000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9019..9039
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9021..9039
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2521 tagaggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2581 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2641 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2701 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2761 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2821 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2881 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2941 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3001 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3061 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3121 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3181 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3241 gcatggacga gctgtacaag accggatcaa caagtttgta caaaaaagca ggctggggct
     3301 cgcaggccct gcccccgggg cccatgcaga ccctcatctt tttcgacatg gaggccactg
     3361 gcttgccctt ctcccagccc aaggtcacgg agctgtgcct gctggctgtc cacagatgtg
     3421 ccctggagag cccccccacc tctcaggggc cacctcccac agttcctcca ccaccgcgtg
     3481 tggtagacaa gctctccctg tgtgtggctc cggggaaggc ctgcagccct gcagccagcg
     3541 agatcacagg tctgagcaca gctgtgctgg cagcgcatgg gcgtcaatgt tttgatgaca
     3601 acctggccaa cctgctccta gccttcctgc ggcgccagcc acagccctgg tgcctggtgg
     3661 cacacaatgg tgaccgctac gacttccccc tgctccaagc agagctggct atgctgggcc
     3721 tcaccagtgc tctggatggt gccttctgtg tggatagcat cactgcgctg aaggccctgg
     3781 agcgagcaag cagcccctca gaacacggcc caaggaagag ctatagccta ggcagcatct
     3841 acactcgcct gtatgggcag tcccctccag actcgcacac ggctgagggt gatgtcctgg
     3901 ccctgctcag catctgtcag tggagaccac aggccctgct gcggtgggtg gatgctcacg
     3961 ccaggccttt cggcaccatc aggcccatgt atggggtctg attagtaatg acaatcaacc
     4021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4141 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4201 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4261 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4321 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4381 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4441 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4501 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4561 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc
     4621 cggttagtaa tgagtttgga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4681 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4741 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4801 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4861 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4921 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4981 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     5041 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     5101 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     5161 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     5221 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     5281 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     5341 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     5401 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     5461 gtgaattgct gccctctggt tatgtgtggg agggctaagc acaattcgag ctcggtacct
     5521 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5581 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5641 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5701 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5761 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5821 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5881 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5941 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6001 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6121 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6181 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6241 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6301 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6361 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6841 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7201 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8761 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8821 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8881 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8941 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9001 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     9061 gcttaa
//
