LOCUS       sequence-326340-        2846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries Golden Gate substitution insert for ER-LBD domain-insertion 
            mapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ho TYH, Shao A, Lu Z, Savilahti H, Menolascina F, Wang L, Dalchau N,
            Wang B
  TITLE     A systematic approach to inserting split inteins for Boolean logic 
            gate engineering and basal activity reduction.
  JOURNAL   Nat Commun. 2021 Apr 13;12(1):2200. doi: 10.1038/s41467-021-22404-9.
  PUBMED    33850130
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-04-13"; volume: "12(1):2200. doi"; pages: " 
            10.1038/s41467-021-22404-9"
COMMENT     Sequence Label: IBMc318
FEATURES             Location/Qualifiers
     source          1..2846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(57..848,849..917))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     680..699
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(918..1022)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1090..1108
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1112..1155)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1158..1179
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1947..1967
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1973..1990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2144..2732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2224..2243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
       61 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      121 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      181 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      241 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      301 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      361 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      421 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      481 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      541 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      601 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      661 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      721 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      781 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      841 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      901 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      961 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1021 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1081 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     1141 gctaaggatg atttctggaa ttcgcggccg cttctagagg aagacgctgg aaatagcctg
     1201 gcactgagcc tgaccgcaga tcagatggtt agcgcactgc tggatgcaga accgcctatt
     1261 ctgtatagcg aatatgatcc gacacgtccg tttagcgaag caagcatgat gggtctgctg
     1321 accaatctgg cagatcgtga actggttcat atgattaatt gggcaaaacg tgttccgggt
     1381 tttgttgatc tgaccctgca tgatcaggtt catctgctgg aatgtgcatg gctggaaatt
     1441 ctgatgattg gtctggtttg gcgtagcatg gaacatccgg gtaaactgct gtttgcaccg
     1501 aatctgctgc tggatcgtaa tcagggtaaa tgtgttgaag gtatggtgga aatctttgat
     1561 atgctgctgg caaccagcag ccgttttcgt atgatgaatc tgcaaggtga agaatttgtt
     1621 tgcctgaaaa gcattattct gctgaacagc ggtgtttata cctttctgag cagcaccctg
     1681 aaaagtctgg aagaaaaaga tcatatccat cgtgtgctgg ataaaatcac cgatacactg
     1741 attcatctga tggcaaaagc cggtctgacc ttacagcagc agcatcagcg tctggcacaa
     1801 ctgctgctga ttctgagcca tattcgtcat atgagcaata aaggtatgga acacctgtac
     1861 agcatgaaat gcaaaaatgt tgttccgctg tatgacctgc tgttagaaat gctggacgca
     1921 catcgtctgc atgcatcaag aagagctact agtagcggcc gctgcaggct tcctcgctca
     1981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
     2161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2761 ctgacgctca gtggaaagaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2821 ggatcttcac ctagatcctt ttaaat
//
