LOCUS       sequence-326345-        8629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0933
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1567..2155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1620..1640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2038..2049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2227..2460
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2538..2659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2575..2594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2629..2648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2699..2834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2854..2873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2855..2873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2883..2900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2883..2899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2892..2914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3041..3496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3128..3147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3338..3359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3522..3626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3627..3695,3696..4487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3845..3864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4658..5246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5147..5166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5400..5417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5534..5555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5570..5575,5576..5593,5594..5600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5608..5624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5613..5635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5632..5648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5632..5648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5667..5687
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5669..5687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5715..5941
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5942..6122
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6169..6294
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6787..7020
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7205..7249
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7480..7690
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7502..7698
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7549..7684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7730..8332
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7730..7749)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8226..8246
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8375..8492
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 accattgaca tttagggatg tggccataga attctctctg aaggagtggc aatgcctgga
     1261 cactgcacag cggaatttat atagaaatgt gatgttagag aactacagaa acctggtctt
     1321 cctgggtatt actgtttcta agccagacct gatcacttgt ctggagcaag ggaaagaggc
     1381 ctggagtatg aagagacatg agatcatggt ggccaaaccc acagaatctt actctgtcac
     1441 ccagtctgga atgcagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     1501 gctttatata tcttgtggaa aggacgatgc gtgctacttc caatcgcacg tacgcgttaa
     1561 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1621 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     1681 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     1741 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     1801 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     1861 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     1921 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     1981 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2041 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2101 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     2161 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     2221 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     2281 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2341 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2401 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2461 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     2521 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2641 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     2701 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2761 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     2821 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     2881 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2941 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3001 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     3061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3481 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3541 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3601 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     3661 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3721 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3781 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3841 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3901 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3961 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4021 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4081 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4141 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4201 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4261 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4321 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4381 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4441 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4501 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4561 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4621 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4681 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4741 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4801 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4861 tatacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4981 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5281 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5341 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5401 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5461 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5521 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     5581 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     5641 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     5701 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     5761 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     5821 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     5881 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     5941 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6001 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6061 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6121 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6181 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6241 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6301 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6361 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6421 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6481 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6541 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6601 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     6661 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     6721 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     6781 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     6841 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     6901 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     6961 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7021 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7081 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7141 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7201 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     7261 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     7321 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     7381 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     7441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7501 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7681 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     7741 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     7801 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     7861 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7921 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     7981 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8041 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8101 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8161 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8221 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8281 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
     8341 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     8401 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     8461 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     8521 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
     8581 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
